FastQCFastQC Report
Thu 26 May 2016
SRR1544759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544759_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences470920
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT36510.7752909199014695Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT28810.6117811942580481Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT19640.4170559755372462Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTTG13730.29155695234859424Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT8910.18920411110167332Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC8400.1783742461564597No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC7840.16648262974602904No Hit
GCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC7110.15098105835386053No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6350.13484243608256177No Hit
AGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC6310.13399303491038816No Hit
ACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT5410.11488150853648177Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA351.0178519E-746.00000423
ATTACGG351.0178519E-746.0000041
TCAGGCG351.0178519E-746.00000441
CCGTAGG351.0178519E-746.0000041
CGGGATA351.0178519E-746.0000045
TACTATT351.0178519E-746.00000445
TCACGAC301.8590472E-646.024
CGGGTAT1150.046.05
CTGTCGG551.8189894E-1246.01
CTCACGG253.414062E-546.01
GCGACGG405.5988494E-946.01
GTCGAAT453.092282E-1046.029
CACGGGC650.046.03
CGACGGT301.8590472E-646.027
ACTTGAA206.307642E-446.024
CCCTACG301.8590472E-646.031
CTATAGG405.5988494E-946.01
ACTCTTG206.307642E-446.028
GCTATTC650.046.038
TAGCGCT206.307642E-446.02
Produced by FastQC (version 0.11.5)