##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544759_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	470920
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93636286418075	34.0	33.0	34.0	31.0	34.0
2	33.26182578781959	34.0	33.0	34.0	31.0	34.0
3	33.343954387157055	34.0	34.0	34.0	31.0	34.0
4	36.58902148984966	37.0	37.0	37.0	35.0	37.0
5	36.57881168776013	37.0	37.0	37.0	35.0	37.0
6	36.54316656756986	37.0	37.0	37.0	35.0	37.0
7	36.4318589144653	37.0	37.0	37.0	35.0	37.0
8	36.47876497069566	37.0	37.0	37.0	35.0	37.0
9	38.26937484073728	39.0	39.0	39.0	37.0	39.0
10	38.06335258642657	39.0	39.0	39.0	35.0	39.0
11	37.132162575384356	39.0	35.0	39.0	35.0	39.0
12	37.01952348594241	39.0	35.0	39.0	35.0	39.0
13	36.982691327614035	39.0	35.0	39.0	35.0	39.0
14	38.127686231207	40.0	35.0	41.0	35.0	41.0
15	38.221952773294824	40.0	35.0	41.0	35.0	41.0
16	38.262354540049266	40.0	35.0	41.0	35.0	41.0
17	38.3771489849656	40.0	36.0	41.0	35.0	41.0
18	38.357113734816956	40.0	36.0	41.0	35.0	41.0
19	38.24107279368045	40.0	35.0	41.0	35.0	41.0
20	38.144383334749	40.0	35.0	41.0	35.0	41.0
21	38.08113267646309	40.0	35.0	41.0	35.0	41.0
22	38.06795421727682	40.0	35.0	41.0	35.0	41.0
23	38.05455279028285	40.0	35.0	41.0	35.0	41.0
24	38.005654888303745	40.0	35.0	41.0	35.0	41.0
25	37.95937314193493	40.0	35.0	41.0	35.0	41.0
26	37.90946869956681	40.0	35.0	41.0	35.0	41.0
27	37.93773677057674	40.0	35.0	41.0	35.0	41.0
28	37.991748067612335	40.0	35.0	41.0	35.0	41.0
29	38.01594750700756	40.0	35.0	41.0	35.0	41.0
30	37.918708060817124	40.0	35.0	41.0	35.0	41.0
31	37.818368300348254	40.0	35.0	41.0	35.0	41.0
32	37.7650152892211	39.0	35.0	41.0	35.0	41.0
33	37.727452645884654	39.0	35.0	41.0	35.0	41.0
34	37.51629363798522	39.0	35.0	41.0	35.0	41.0
35	37.55494776182791	39.0	35.0	41.0	35.0	41.0
36	37.42809606727257	39.0	35.0	41.0	35.0	41.0
37	37.37733797672641	39.0	35.0	41.0	35.0	41.0
38	37.34172895608596	38.0	35.0	41.0	35.0	41.0
39	37.33507602140491	39.0	35.0	41.0	35.0	41.0
40	37.35106599847108	39.0	35.0	41.0	35.0	41.0
41	37.35071986749342	39.0	35.0	41.0	35.0	41.0
42	37.31711118661344	39.0	35.0	41.0	35.0	41.0
43	37.26800093434129	39.0	35.0	41.0	35.0	41.0
44	37.142096322092925	38.0	35.0	41.0	35.0	41.0
45	37.12810668478723	38.0	35.0	41.0	35.0	41.0
46	37.05180285398794	38.0	35.0	41.0	34.0	41.0
47	37.02846343327954	38.0	35.0	41.0	34.0	41.0
48	37.018034910388174	37.0	35.0	41.0	34.0	41.0
49	36.96720674424531	37.0	35.0	41.0	34.0	41.0
50	36.89686570967468	37.0	35.0	41.0	34.0	41.0
51	36.83060817123928	37.0	35.0	41.0	34.0	41.0
52	36.466741697103544	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	0.0
15	10.0
16	11.0
17	15.0
18	29.0
19	66.0
20	100.0
21	201.0
22	308.0
23	505.0
24	805.0
25	1377.0
26	1884.0
27	2183.0
28	2162.0
29	2329.0
30	2748.0
31	3363.0
32	4833.0
33	7546.0
34	26269.0
35	130894.0
36	13963.0
37	20646.0
38	41607.0
39	206363.0
40	698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.37012656077465	21.81389620317676	24.40796738299499	23.408009853053596
2	24.987896033296526	24.9275885500722	27.15939012995838	22.925125286672895
3	26.072368979869193	25.406226110592034	26.845748747133268	21.675656162405506
4	25.38668988363204	26.896288116877603	25.489892126051135	22.227129873439225
5	24.239361250318524	33.95566125881253	21.695617089951586	20.109360400917353
6	85.10702454769388	2.7943175061581584	8.517157903677909	3.5815000424700587
7	86.57330332115858	1.9931198505053938	6.227172343497834	5.206404484838189
8	76.883547099295	3.7484073728021747	12.299328973073981	7.068716554828845
9	53.989000254820354	14.017455194088168	17.182748662193152	14.810795888898326
10	49.37420368640109	16.623205640023784	22.78072708740338	11.221863586171748
11	43.699354455109145	17.488320733882613	22.106514906990572	16.70580990401767
12	18.024929924403295	40.71731928990062	25.99507347320139	15.262677312494692
13	12.093773889407968	43.8458761573091	27.70088337721906	16.359466576063873
14	10.3654548543277	22.9168436252442	50.45634077975028	16.261360740677823
15	10.98615476089357	16.106345026756134	53.407585152467504	19.499915059882785
16	12.307398284209633	19.238511849146352	27.45115943260002	41.002930434043996
17	17.994563832498088	22.35347829780005	35.93243013675359	23.71952773294827
18	25.332965259492056	25.959610974263143	27.50743226025652	21.199991505988276
19	28.952688354709927	21.842988193323706	23.91849995752994	25.28582349443642
20	17.527817888388686	32.262804722670516	27.211415951754013	22.997961437186785
21	18.883462159177782	24.1790537670942	25.689713751804977	31.247770321923046
22	15.669540473965855	32.533338996007814	21.96211670772106	29.83500382230527
23	16.971672470908008	22.278306294062684	40.1834706531895	20.566550581839806
24	16.340567399983012	21.73277839123418	37.04535802259407	24.881296186188738
25	13.260214049095387	34.85815000424701	28.24407542682409	23.637560519833517
26	16.057504459356153	31.1477533338996	27.390214898496563	25.404527308247687
27	17.41399813131742	26.47010107873949	38.13577677737195	17.98012401257114
28	14.085831988448144	23.757963135989126	34.863034060987005	27.293170814575724
29	18.14172258557717	27.274908689373987	27.807058523740764	26.77631020130808
30	29.1820266711968	27.343922534613096	20.41365837084855	23.060392423341543
31	29.534740507941905	23.52098020895269	22.039199864095814	24.9050794190096
32	27.406565871060902	26.820479062261104	22.9701435487981	22.802811517879896
33	19.41922194852629	25.43531810073898	26.43315212774994	28.712307822984794
34	18.787692177015206	22.343710184320052	32.6575639174382	26.211033721226535
35	34.48674934171409	18.980293892805573	24.811220589484414	21.72173617599592
36	23.077592797078058	25.689713751804977	30.30493502081033	20.927758430306636
37	22.78391234179903	28.472351991845752	26.74148475324896	22.002250913106263
38	20.881466066423172	26.475622186358617	28.471077890087486	24.17183385713072
39	28.800433194597808	20.7549052917693	26.503015374161215	23.941646139471672
40	17.332455618788753	18.73757750785696	31.70920750870636	32.22075936464792
41	22.482374925677398	23.896415527053428	23.40439989807186	30.21680964919732
42	23.38571307228404	22.681347150259068	26.828123672810666	27.104816104646222
43	24.123417990316824	26.46266881848297	27.02518474475495	22.388728446445256
44	17.860995498173786	31.58285908434554	24.760893570033126	25.79525184744755
45	23.487004162065745	27.107789008748835	26.096152212690054	23.30905461649537
46	24.50033976046887	20.551686061326766	28.86689883632039	26.08107534188397
47	23.622271298734393	17.389577847617428	33.657309097086554	25.33084175656162
48	20.21511084685297	15.804595260341458	32.35262889662788	31.627664996177696
49	20.449545570372887	24.503737365157562	31.47583453665166	23.570882527817886
50	19.506073218381044	27.243480846003564	27.153231971460123	26.09721396415527
51	20.812664571477107	19.70738129618619	31.578399728191624	27.90155440414508
52	24.715238257028798	18.503992185509215	33.61462668818483	23.16614286927716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	111.5
2	112.0
3	445.0
4	778.0
5	611.5
6	445.0
7	544.5
8	644.0
9	706.5
10	769.0
11	728.5
12	688.0
13	694.5
14	637.5
15	574.0
16	578.5
17	583.0
18	533.0
19	483.0
20	510.5
21	538.0
22	655.0
23	772.0
24	893.5
25	1015.0
26	1144.0
27	1273.0
28	1610.0
29	1947.0
30	2329.5
31	2712.0
32	3160.5
33	3609.0
34	4401.0
35	5193.0
36	5950.5
37	6708.0
38	7273.5
39	9576.0
40	11313.0
41	17887.5
42	24462.0
43	34193.5
44	43925.0
45	48558.0
46	53191.0
47	51424.5
48	49658.0
49	54964.0
50	60270.0
51	61640.5
52	63011.0
53	53827.0
54	44643.0
55	36049.0
56	27455.0
57	21680.5
58	15906.0
59	14142.5
60	12379.0
61	11063.5
62	9748.0
63	8395.0
64	5575.0
65	4108.0
66	3107.5
67	2107.0
68	1760.0
69	1413.0
70	1223.0
71	1033.0
72	807.5
73	582.0
74	513.5
75	445.0
76	439.5
77	434.0
78	306.5
79	179.0
80	130.5
81	82.0
82	58.5
83	35.0
84	20.0
85	5.0
86	2.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	470920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.569736026012684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.07606697468492	17.662567982578665
2	12.779527818834383	6.535383057293616
3	4.857975384132231	3.7265144457938613
4	2.39731155164691	2.451944941908895
5	1.3755635128021475	1.758639795468282
6	0.8071114127653218	1.2382575460794867
7	0.576792826054132	1.0323908212731403
8	0.41886911577369706	0.8568298175826223
9	0.3349268107581718	0.7707591125208685
>10	6.686056377337726	46.06290979811688
>50	0.5493453256072773	9.395719122083996
>100	0.1313109855460663	5.3413262797661645
>500	0.005817575309002937	1.0695402890814842
>1k	0.003324328748001678	2.09721699045205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	3651	0.7752909199014695	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT	2881	0.6117811942580481	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1964	0.4170559755372462	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTTG	1373	0.29155695234859424	Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	891	0.18920411110167332	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	840	0.1783742461564597	No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	784	0.16648262974602904	No Hit
GCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	711	0.15098105835386053	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	635	0.13484243608256177	No Hit
AGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	631	0.13399303491038816	No Hit
ACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	541	0.11488150853648177	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6302556697528242	0.0
2	0.0	0.0	0.0	2.167247090800985	0.0
3	0.0	0.0	0.0	3.3067187632718933	0.0
4	0.0	0.0	0.0	4.011297035589909	0.0
5	0.0	0.0	0.0	7.550751720037374	0.0
6	0.0	0.0	0.0	8.163169965174552	0.0
7	0.0	0.0	0.0	10.664656417225856	0.0
8	0.0	0.0	0.0	13.292703643931029	0.0
9	0.0	0.0	0.0	15.602862481950226	0.0
10	0.0	0.0	0.0	16.864647923214132	0.0
11	0.0	0.0	0.0	18.035547439055467	0.0
12	0.0	0.0	0.0	19.18351312324811	0.0
13	0.0	0.0	0.0	19.573388261275802	0.0
14	0.0	0.0	0.0	19.89955831139047	0.0
15	0.0	0.0	0.0	21.045612842945722	0.0
16	0.0	0.0	0.0	22.192941476259236	0.0
17	0.0	0.0	0.0	23.98772615306209	0.0
18	0.0	0.0	0.0	24.591225685891448	0.0
19	0.0	0.0	0.0	25.340185169455534	0.0
20	0.0	0.0	0.0	26.29363798522042	0.0
21	0.0	0.0	0.0	27.02603414592712	0.0
22	2.123502930434044E-4	0.0	0.0	27.979911662278095	0.0
23	4.247005860868088E-4	0.0	0.0	28.58404824598658	0.0
24	6.370508791302132E-4	0.0	0.0	29.073940372037715	0.0
25	6.370508791302132E-4	0.0	0.0	29.62307822984796	0.0
26	6.370508791302132E-4	0.0	0.0	30.060095132931284	0.0
27	6.370508791302132E-4	0.0	0.0	30.521107619128514	0.0
28	6.370508791302132E-4	0.0	0.0	30.92393612503185	0.0
29	6.370508791302132E-4	0.0	0.0	31.577337976726408	0.0
30	6.370508791302132E-4	0.0	0.0	32.19548967977576	0.0
31	6.370508791302132E-4	0.0	0.0	32.636965939012995	0.0
32	6.370508791302132E-4	0.0	0.0	33.07886689883632	0.0
33	6.370508791302132E-4	0.0	0.0	33.51758260426399	0.0
34	6.370508791302132E-4	0.0	0.0	34.20389875138028	0.0
35	6.370508791302132E-4	0.0	0.0	34.66873354285229	0.0
36	6.370508791302132E-4	0.0	0.0	35.10596279622866	0.0
37	6.370508791302132E-4	0.0	0.0	35.52726577762677	0.0
38	6.370508791302132E-4	0.0	0.0	35.89144653019621	0.0
39	6.370508791302132E-4	0.0	0.0	36.35288371697953	0.0
40	6.370508791302132E-4	0.0	0.0	36.81601970610719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	35	1.0178519E-7	46.000004	23
ATTACGG	35	1.0178519E-7	46.000004	1
TCAGGCG	35	1.0178519E-7	46.000004	41
CCGTAGG	35	1.0178519E-7	46.000004	1
CGGGATA	35	1.0178519E-7	46.000004	5
TACTATT	35	1.0178519E-7	46.000004	45
TCACGAC	30	1.8590472E-6	46.0	24
CGGGTAT	115	0.0	46.0	5
CTGTCGG	55	1.8189894E-12	46.0	1
CTCACGG	25	3.414062E-5	46.0	1
GCGACGG	40	5.5988494E-9	46.0	1
GTCGAAT	45	3.092282E-10	46.0	29
CACGGGC	65	0.0	46.0	3
CGACGGT	30	1.8590472E-6	46.0	27
ACTTGAA	20	6.307642E-4	46.0	24
CCCTACG	30	1.8590472E-6	46.0	31
CTATAGG	40	5.5988494E-9	46.0	1
ACTCTTG	20	6.307642E-4	46.0	28
GCTATTC	65	0.0	46.0	38
TAGCGCT	20	6.307642E-4	46.0	2
>>END_MODULE