FastQCFastQC Report
Thu 26 May 2016
SRR1544758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544758_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences665182
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT32630.490542438009447No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT30930.4649855227591847Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT22350.3359982681431548No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.20355331322856No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC10370.15589718302660024No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT9750.14657642570003396No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC9100.13680466398669838No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8890.13364763327931303No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC8300.12477788033951612No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8120.1220718540189001No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT7320.1100450703717178No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT6950.10448268293489602No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC6700.10072431304515156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG301.8602113E-646.018
CGGGTAT1200.046.05
GGTACGA253.415592E-546.07
ACACGTG1250.046.041
ACCGGGT301.8602113E-646.03
GGTCGAA206.309527E-446.07
GTTTCGG253.415592E-546.01
GCGAAAC206.309527E-446.08
GACGTGA301.8602113E-646.09
GAATCGG206.309527E-446.01
ACCTGAT206.309527E-446.020
ATTCGTC253.415592E-546.016
CTAACGG453.092282E-1046.01
CGTAGGT206.309527E-446.023
CCATAGG453.092282E-1046.01
TCGTCAG253.415592E-546.018
CGGGACC850.046.05
GCGATGA850.046.08
TTAACGG501.6370905E-1146.01
GCGATAC405.6061253E-946.08
Produced by FastQC (version 0.11.5)