##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544758_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	665182
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.918014919225115	34.0	33.0	34.0	31.0	34.0
2	33.23034748384653	34.0	33.0	34.0	31.0	34.0
3	33.2749217507389	34.0	34.0	34.0	31.0	34.0
4	36.562412993737055	37.0	37.0	37.0	35.0	37.0
5	36.56308499027334	37.0	37.0	37.0	35.0	37.0
6	36.67469805256306	37.0	37.0	37.0	35.0	37.0
7	36.64578566467523	37.0	37.0	37.0	35.0	37.0
8	36.54649855227592	37.0	37.0	37.0	35.0	37.0
9	38.33928759347066	39.0	39.0	39.0	37.0	39.0
10	38.11394024492545	39.0	39.0	39.0	35.0	39.0
11	37.425187392322705	39.0	35.0	39.0	35.0	39.0
12	37.329975856231826	39.0	35.0	39.0	35.0	39.0
13	37.30804050620733	39.0	35.0	39.0	35.0	39.0
14	38.59112543634674	41.0	35.0	41.0	35.0	41.0
15	38.66004642338488	41.0	35.0	41.0	35.0	41.0
16	38.65168630540213	41.0	35.0	41.0	35.0	41.0
17	38.72411460322137	41.0	36.0	41.0	35.0	41.0
18	38.70533177386039	41.0	36.0	41.0	35.0	41.0
19	38.62729448481769	41.0	35.0	41.0	35.0	41.0
20	38.536916212405025	40.0	35.0	41.0	35.0	41.0
21	38.48075714616451	40.0	35.0	41.0	35.0	41.0
22	38.469110709550165	40.0	35.0	41.0	35.0	41.0
23	38.43653315934586	40.0	35.0	41.0	35.0	41.0
24	38.37956829860099	40.0	35.0	41.0	35.0	41.0
25	38.350627347102	40.0	35.0	41.0	35.0	41.0
26	38.318173071430074	40.0	35.0	41.0	35.0	41.0
27	38.3212263711285	40.0	35.0	41.0	35.0	41.0
28	38.33867573085261	40.0	35.0	41.0	35.0	41.0
29	38.3260806816781	40.0	36.0	41.0	35.0	41.0
30	38.25293378353594	40.0	35.0	41.0	35.0	41.0
31	38.19795785213671	40.0	35.0	41.0	35.0	41.0
32	38.09766048991103	40.0	35.0	41.0	35.0	41.0
33	38.03269781804077	40.0	35.0	41.0	35.0	41.0
34	37.9198084133365	40.0	35.0	41.0	35.0	41.0
35	37.84495220856848	40.0	35.0	41.0	35.0	41.0
36	37.803990486814136	40.0	35.0	41.0	35.0	41.0
37	37.71658282996233	40.0	35.0	41.0	35.0	41.0
38	37.693853712217106	40.0	35.0	41.0	35.0	41.0
39	37.59088339732585	40.0	35.0	41.0	35.0	41.0
40	37.52652958137773	40.0	35.0	41.0	34.0	41.0
41	37.48719598545962	39.0	35.0	41.0	34.0	41.0
42	37.408174003505806	39.0	35.0	41.0	34.0	41.0
43	37.41672053663508	39.0	35.0	41.0	34.0	41.0
44	37.3914131771455	39.0	35.0	41.0	34.0	41.0
45	37.38761872690482	39.0	35.0	41.0	35.0	41.0
46	37.36136726489893	39.0	35.0	41.0	35.0	41.0
47	37.27992639608408	39.0	35.0	41.0	35.0	41.0
48	37.26745762813786	39.0	35.0	41.0	34.0	41.0
49	37.207508621700526	39.0	35.0	41.0	34.0	41.0
50	37.14626072262929	39.0	35.0	41.0	34.0	41.0
51	37.081513029516735	38.0	35.0	41.0	34.0	41.0
52	36.83912523189142	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	3.0
15	11.0
16	8.0
17	14.0
18	48.0
19	68.0
20	144.0
21	231.0
22	407.0
23	690.0
24	1221.0
25	2140.0
26	2785.0
27	3361.0
28	3424.0
29	3778.0
30	4464.0
31	5477.0
32	7396.0
33	10820.0
34	32022.0
35	139946.0
36	23762.0
37	35314.0
38	63532.0
39	320888.0
40	3225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.57213815166375	22.293146838008244	25.041417236184984	20.093297774143014
2	27.232546881906007	24.168573412990728	26.741854109100966	21.8570255960023
3	27.140992991391826	24.030415735843725	26.322269694609897	22.50632157815455
4	25.63599135274256	27.206538962268972	24.233217375094334	22.924252309894133
5	24.302070711474457	35.08378158158219	20.791903569248717	19.822244137694646
6	86.33847578557447	2.927770144110935	7.885661367866239	2.8480927024483527
7	87.45050226855207	2.5726793569278783	5.4781999512915265	4.49861842322853
8	77.31688470223187	5.144306370286628	10.851315880465798	6.687493047015704
9	49.25854878815122	22.463025157024692	14.103508513459474	14.174917541364618
10	41.44745347889751	19.88072437317907	25.89997925379821	12.771842894125218
11	39.26669693407218	18.290783575021575	24.386709201391497	18.05581028951475
12	20.87082933693335	34.29332122637113	27.09844824423992	17.7374011924556
13	14.422518949700985	36.61418980068613	29.355274195633672	19.608017053979214
14	13.274261780986258	24.95572640269881	43.34407726005815	18.425934556256784
15	15.580096875742278	18.89858715359105	45.2214581873833	20.29985778328337
16	15.162015809207105	22.788800659067743	27.83298405549158	34.21619947623357
17	19.33125069529843	24.02079430892598	32.25838943326789	24.389565562507702
18	22.81315489595329	28.48904510344537	27.613645588726094	21.084154411875247
19	27.011855401980213	23.338124002152792	25.895769879521698	23.7542507163453
20	19.516312828669445	30.00832854767567	27.591546373774396	22.883812249880485
21	19.531797312615193	24.755330120177636	27.111828041047414	28.601044526159757
22	18.440366696633404	29.161642978914042	24.03417410573347	28.36381621871909
23	19.26314903289626	22.40379324756232	37.004458930037195	21.328598789504227
24	18.99089271808317	22.775871866647023	33.780228569023215	24.45300684624659
25	15.10203222576678	31.838354014390045	29.241470755372212	23.818143004470958
26	15.969463996319805	29.028145680430317	30.26946008761512	24.732930235634758
27	18.619716107772007	26.451256949225925	36.66921233587199	18.259814607130078
28	16.33041784053086	24.545011741147533	33.034267313306735	26.09030310501487
29	18.500801284460493	26.771019059445383	31.914122751367298	22.814056904726826
30	25.643508092522048	25.49332363172786	26.133298856553544	22.72986941919655
31	28.009627440309572	26.04204563563055	24.774723308808717	21.173603615251164
32	22.03171462847762	26.987801834685843	29.58182873258747	21.398654804249063
33	18.588145800698154	27.540883547660638	29.454344825927343	24.416625825713865
34	19.292464318036266	23.70794759930365	31.517088556214688	25.482499526445395
35	25.62757260418953	26.418032959400584	27.78352390774254	20.170870528667344
36	22.374026958035547	27.716173919318322	28.793172394923495	21.116626727722636
37	19.605311027658594	29.42968991945062	27.89822935677754	23.066769696113244
38	20.34135018686615	28.93403609839112	25.889155148515748	24.835458566226986
39	25.71687147276986	24.89469047568936	27.055001488314478	22.333436563226307
40	17.385016431593158	21.43969620344507	32.92843161721153	28.24685574775024
41	21.979849123999145	24.56139823386682	25.655835545760407	27.802917096373626
42	22.17363067551437	22.45340373010695	29.287773872413865	26.08519172196482
43	23.359471543126542	26.518306268058968	27.97595244609746	22.14626974271703
44	17.04450811958231	29.81349465259132	27.47413489841878	25.667862329407594
45	20.738384382018758	27.742933512933305	27.10070326617377	24.41797883887417
46	22.780381910514716	23.562423517172743	28.57849430682129	25.07870026549125
47	21.275981611047804	21.280491654915494	33.58855170464625	23.854975029390452
48	20.758228575036604	19.958597797294576	29.94924697300889	29.333926654659926
49	19.976487637969758	24.126930674612364	32.23328352240439	23.663298165013487
50	18.75997847205727	26.118415711790156	29.071592436355765	26.050013379796805
51	20.214768288979556	21.2583924399638	30.562161934628417	27.964677336428224
52	21.33355983775869	19.993926474258174	33.68326262586781	24.98925106211533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	245.0
2	344.0
3	1402.5
4	2461.0
5	1667.5
6	874.0
7	933.0
8	992.0
9	1049.5
10	1107.0
11	1067.5
12	1028.0
13	1070.0
14	1044.0
15	976.0
16	900.0
17	824.0
18	813.0
19	802.0
20	978.5
21	1155.0
22	1329.0
23	1503.0
24	1611.5
25	1720.0
26	2243.0
27	2766.0
28	4091.5
29	5417.0
30	5995.5
31	6574.0
32	7730.0
33	8886.0
34	10832.0
35	12778.0
36	13210.0
37	13642.0
38	15663.0
39	19940.0
40	22196.0
41	31157.5
42	40119.0
43	54413.5
44	68708.0
45	80108.0
46	91508.0
47	90342.0
48	89176.0
49	76724.0
50	64272.0
51	54520.0
52	44768.0
53	39379.0
54	33990.0
55	29761.5
56	25533.0
57	23848.0
58	22163.0
59	21281.5
60	20400.0
61	19978.0
62	19556.0
63	16742.5
64	11490.5
65	9052.0
66	7234.5
67	5417.0
68	4696.0
69	3975.0
70	3461.5
71	2948.0
72	2602.0
73	2256.0
74	1667.5
75	1079.0
76	891.0
77	703.0
78	503.0
79	303.0
80	232.5
81	162.0
82	139.0
83	116.0
84	81.5
85	47.0
86	29.5
87	12.0
88	6.5
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	665182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.56131887252923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.94177321775558	18.311844220744018
2	12.680632849839926	6.736286652601349
3	4.772308984601198	3.8027646209468586
4	2.3201102241255156	2.4650074992965236
5	1.3081766561736832	1.737344865311412
6	0.8431621732327973	1.3437299606694637
7	0.5772011821027179	1.0731857256001776
8	0.4774222989456789	1.0144772735321725
9	0.37516495584054105	0.8968388419691063
>10	7.14181735348724	47.33428708302859
>50	0.4504648864710046	7.794993619863602
>100	0.10268783935925797	4.598817183942838
>500	0.006240697419623412	1.2355233662477008
>1k	0.0028366806452833696	1.6548990862461581
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	3263	0.490542438009447	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT	3093	0.4649855227591847	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	2235	0.3359982681431548	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	0.20355331322856	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1037	0.15589718302660024	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	975	0.14657642570003396	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	910	0.13680466398669838	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	889	0.13364763327931303	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	830	0.12477788033951612	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	812	0.1220718540189001	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	732	0.1100450703717178	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	695	0.10448268293489602	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	670	0.10072431304515156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4734042713122123	0.0
2	0.0	0.0	0.0	1.5700966051396459	0.0
3	0.0	0.0	0.0	2.500217985453605	0.0
4	0.0	0.0	0.0	3.09419076282882	0.0
5	0.0	0.0	0.0	5.975507455102513	0.0
6	0.0	0.0	0.0	6.453271435486829	0.0
7	0.0	0.0	0.0	8.351699234194552	0.0
8	0.0	0.0	0.0	10.64580821489457	0.0
9	0.0	0.0	0.0	12.504096623179821	0.0
10	0.0	0.0	0.0	13.654608813828396	0.0
11	0.0	0.0	0.0	14.78512647666353	0.0
12	0.0	0.0	0.0	15.785905210904685	0.0
13	0.0	0.0	0.0	16.16986027884098	0.0
14	0.0	0.0	0.0	16.483909666828026	0.0
15	0.0	0.0	0.0	17.45191541563061	0.0
16	0.0	0.0	0.0	18.565896250950868	0.0
17	0.0	0.0	0.0	20.128776785902204	0.0
18	0.0	0.0	0.0	20.81911416725047	0.0
19	0.0	0.0	0.0	21.638438803214758	0.0
20	0.0	0.0	0.0	22.562997796091896	0.0
21	0.0	0.0	0.0	23.38187142766942	0.0
22	0.0	0.0	0.0	24.287638571097833	0.0
23	0.0	0.0	0.0	24.93738555763686	0.0
24	1.5033479558977843E-4	0.0	0.0	25.51301748995012	0.0
25	1.5033479558977843E-4	0.0	0.0	26.04835969704532	0.0
26	1.5033479558977843E-4	0.0	0.0	26.553935614613746	0.0
27	1.5033479558977843E-4	0.0	0.0	27.063119567276324	0.0
28	1.5033479558977843E-4	0.0	0.0	27.5753102158507	0.0
29	1.5033479558977843E-4	0.0	0.0	28.278877059210863	0.0
30	1.5033479558977843E-4	0.0	0.0	28.90998253109675	0.0
31	1.5033479558977843E-4	0.0	0.0	29.489673502890938	0.0
32	1.5033479558977843E-4	0.0	0.0	30.021708344483162	0.0
33	1.5033479558977843E-4	0.0	0.0	30.516610491564716	0.0
34	1.5033479558977843E-4	0.0	0.0	31.224236374405802	0.0
35	1.5033479558977843E-4	0.0	0.0	31.73627668818459	0.0
36	1.5033479558977843E-4	0.0	0.0	32.28860672718143	0.0
37	1.5033479558977843E-4	0.0	0.0	32.82079190356925	0.0
38	1.5033479558977843E-4	0.0	0.0	33.33583891325983	0.0
39	1.5033479558977843E-4	0.0	0.0	33.91342519791576	0.0
40	1.5033479558977843E-4	0.0	0.0	34.51130667997631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	30	1.8602113E-6	46.0	18
CGGGTAT	120	0.0	46.0	5
GGTACGA	25	3.415592E-5	46.0	7
ACACGTG	125	0.0	46.0	41
ACCGGGT	30	1.8602113E-6	46.0	3
GGTCGAA	20	6.309527E-4	46.0	7
GTTTCGG	25	3.415592E-5	46.0	1
GCGAAAC	20	6.309527E-4	46.0	8
GACGTGA	30	1.8602113E-6	46.0	9
GAATCGG	20	6.309527E-4	46.0	1
ACCTGAT	20	6.309527E-4	46.0	20
ATTCGTC	25	3.415592E-5	46.0	16
CTAACGG	45	3.092282E-10	46.0	1
CGTAGGT	20	6.309527E-4	46.0	23
CCATAGG	45	3.092282E-10	46.0	1
TCGTCAG	25	3.415592E-5	46.0	18
CGGGACC	85	0.0	46.0	5
GCGATGA	85	0.0	46.0	8
TTAACGG	50	1.6370905E-11	46.0	1
GCGATAC	40	5.6061253E-9	46.0	8
>>END_MODULE