FastQCFastQC Report
Thu 26 May 2016
SRR1544757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544757_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences497070
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT42120.8473655621944595No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT37880.7620657050314844Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT24360.49007182087029993No Hit
TGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTTG19060.38344699941658117Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14660.29492827971915425No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC10530.21184139054861487No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC9270.18649284808980626No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT9170.18448105900577383No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC8690.17482447140241816No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC7610.15309714929486792No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT7110.14303820387470575No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.1263403544772366No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5660.11386726215623555No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG5150.10360713782767014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTG551.8189894E-1246.0000041
TGACTAT551.8189894E-1246.00000423
GTCATCG206.30798E-446.043
ACCGGTT206.30798E-446.013
TTCACGG253.414338E-546.01
CCGTGAC206.30798E-446.031
CGCGCCA253.414338E-546.012
TAAGTGG206.30798E-446.01
TATGACT206.30798E-446.027
TGCGCTC253.414338E-546.023
TACGCGC206.30798E-446.034
ATGACTA301.8592564E-646.013
ATGACGG600.046.01
AAGATTC206.30798E-446.026
CCAACGG206.30798E-446.01
TCGCGCC253.414338E-546.011
TCAACGG206.30798E-446.01
CCACCGG253.414338E-546.01
GACTATA206.30798E-446.08
TCGATGG351.0179974E-745.9999961
Produced by FastQC (version 0.11.5)