##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544757_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	497070
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92300078459774	34.0	33.0	34.0	31.0	34.0
2	33.26411974168628	34.0	33.0	34.0	31.0	34.0
3	33.34789667451265	34.0	34.0	34.0	31.0	34.0
4	36.584392540286075	37.0	37.0	37.0	35.0	37.0
5	36.58584505200475	37.0	37.0	37.0	35.0	37.0
6	36.52880881968334	37.0	37.0	37.0	35.0	37.0
7	36.39042589574909	37.0	37.0	37.0	35.0	37.0
8	36.44206650974712	37.0	37.0	37.0	35.0	37.0
9	38.29382984287927	39.0	39.0	39.0	37.0	39.0
10	38.15396020681192	39.0	39.0	39.0	35.0	39.0
11	37.567938117367774	39.0	35.0	39.0	35.0	39.0
12	37.52567847586859	39.0	35.0	39.0	35.0	39.0
13	37.51108495785302	39.0	35.0	39.0	35.0	39.0
14	38.786034160178644	41.0	35.0	41.0	35.0	41.0
15	38.85014786649768	41.0	35.0	41.0	35.0	41.0
16	38.85661576840284	41.0	35.0	41.0	35.0	41.0
17	38.93719798016376	41.0	36.0	41.0	35.0	41.0
18	38.92552356810912	41.0	36.0	41.0	35.0	41.0
19	38.86109602269298	41.0	36.0	41.0	35.0	41.0
20	38.7888245116382	41.0	35.0	41.0	35.0	41.0
21	38.73823203975295	41.0	35.0	41.0	35.0	41.0
22	38.73339167521677	41.0	35.0	41.0	35.0	41.0
23	38.7156356247611	41.0	35.0	41.0	35.0	41.0
24	38.64705977025368	41.0	35.0	41.0	35.0	41.0
25	38.60912949886334	41.0	35.0	41.0	35.0	41.0
26	38.562689359647536	41.0	35.0	41.0	35.0	41.0
27	38.570251674814415	40.0	36.0	41.0	35.0	41.0
28	38.61715854909771	40.0	36.0	41.0	35.0	41.0
29	38.63199549359245	40.0	36.0	41.0	35.0	41.0
30	38.58485927535357	40.0	35.0	41.0	35.0	41.0
31	38.558370048484115	41.0	35.0	41.0	35.0	41.0
32	38.48730963445792	40.0	35.0	41.0	35.0	41.0
33	38.44289134327157	40.0	35.0	41.0	35.0	41.0
34	38.35499024282294	40.0	35.0	41.0	35.0	41.0
35	38.29165308709035	40.0	35.0	41.0	35.0	41.0
36	38.27269398676243	40.0	35.0	41.0	35.0	41.0
37	38.209745106323055	40.0	35.0	41.0	35.0	41.0
38	38.1735791739594	40.0	35.0	41.0	35.0	41.0
39	38.13281429174965	40.0	35.0	41.0	35.0	41.0
40	38.1055565614501	40.0	35.0	41.0	35.0	41.0
41	38.0751664755467	40.0	35.0	41.0	35.0	41.0
42	38.01503409982497	40.0	35.0	41.0	35.0	41.0
43	37.94846399903434	40.0	35.0	41.0	35.0	41.0
44	37.834270827046495	40.0	35.0	41.0	35.0	41.0
45	37.82558190999256	40.0	35.0	41.0	35.0	41.0
46	37.80740740740741	40.0	35.0	41.0	35.0	41.0
47	37.7384774780212	40.0	35.0	41.0	35.0	41.0
48	37.699726396684575	40.0	35.0	41.0	35.0	41.0
49	37.65798579676907	39.0	35.0	41.0	35.0	41.0
50	37.60350654837347	39.0	35.0	41.0	35.0	41.0
51	37.551399199307944	39.0	35.0	41.0	35.0	41.0
52	37.16900838916048	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	2.0
15	3.0
16	7.0
17	18.0
18	29.0
19	54.0
20	86.0
21	147.0
22	213.0
23	433.0
24	693.0
25	1132.0
26	1659.0
27	1821.0
28	1855.0
29	2036.0
30	2522.0
31	3123.0
32	4269.0
33	6461.0
34	19815.0
35	97317.0
36	16221.0
37	25660.0
38	46880.0
39	261067.0
40	3543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.551692920514213	22.278954674391937	23.827629911280102	22.341722493813748
2	26.624016738085178	24.375842436678937	26.776107992838032	22.224032832397853
3	27.09115416339751	23.94713018287163	26.324260164564343	22.637455489166516
4	25.650713179230287	26.47091958879031	24.67479429456616	23.20357293741324
5	24.05938801376064	34.058180940310216	21.18554730722031	20.696883738708834
6	83.68599995976422	2.9072364053352646	9.365280543987769	4.041483090912749
7	84.89307341018367	2.5445108334842175	6.693021103667491	5.869394652664615
8	73.30718007524091	5.195445309513751	13.195324602168709	8.302050013076629
9	46.98855292011185	21.540024543826824	15.46281207878166	16.00861045727966
10	38.74243064357133	19.321423541955863	28.606836059307543	13.32930975516527
11	36.379383185466835	18.089001549077594	25.60484438811435	19.92677087734122
12	20.180256301929305	31.859295471462772	29.12104934918623	18.83939887742169
13	14.158368036695032	34.67439193674935	30.48323173798459	20.684008288571025
14	12.869213591647052	24.91057597521476	41.982819321222365	20.237391111915827
15	14.736556219445953	19.070352264268614	44.58044138652504	21.612650129760397
16	14.517070030378015	24.038666586195102	28.005914659907056	33.43834872351983
17	17.970104814211275	24.32454181503611	32.427625887701936	25.277727483050676
18	21.439636268533608	28.808014967710786	28.50986782545718	21.242480938298428
19	25.15762367473394	23.02170720421671	26.456233528476876	25.364435592572477
20	18.4865310720824	30.817188725933974	27.7667129378156	22.929567264168025
21	18.731365803609147	24.717041865330838	27.380248254772972	29.171344076287042
22	17.76248818073913	29.293258494779405	23.966845715895147	28.977407608586315
23	18.185165067294346	22.44291548474058	36.95475486350011	22.417164584464967
24	19.467881787273424	22.563622829782524	33.004003460277225	24.964491922666827
25	15.088216951334822	30.698895527792867	29.574707787635546	24.63817973323677
26	16.224676604904744	27.347053734886433	30.32027682217796	26.107992838030864
27	19.714325950067398	25.284366387027983	36.310982356609735	18.690325306294888
28	17.048303055907617	23.975496408956484	32.34393546180618	26.632265073329712
29	18.865753314422516	25.633210614199207	31.992274729917312	23.508761341460964
30	24.49312974027803	25.419156255658155	26.587603355664193	23.500110648399623
31	28.14593518015571	25.061661335425594	24.405415736214213	22.38698774820448
32	21.194600358098455	25.994125575874627	30.82825356589615	21.983020500130763
33	18.789506508137688	26.801255356388438	29.936427464944575	24.472810670529302
34	19.989337517854626	21.70418653308387	32.15985676061722	26.14661918844428
35	24.892671052366868	26.407749411551695	28.428189188645465	20.271390347435975
36	23.339569879493833	26.95716901040095	28.16565071317923	21.537610396925984
37	19.594825678475868	28.986259480556058	28.725933973082263	22.69298086788581
38	20.504154344458527	28.295209930190918	25.0373186875088	26.163317037841754
39	26.464884221538217	23.91192387390106	27.24304423924196	22.380147665318766
40	17.71279699036353	20.530106423642543	33.124308447502365	28.63278813849156
41	22.21095620335164	24.132214778602613	25.454966101353932	28.201862916691816
42	22.010179652765206	20.862051622507895	30.399340133180434	26.72842859154646
43	24.71402418170479	24.829903232945057	28.090208622528014	22.36586396282214
44	17.438388959301506	27.370591667169613	28.763353250045263	26.42766612348361
45	20.173416219043595	26.241776812119017	27.840746776107995	25.744060192729396
46	23.572937413241597	21.633975094051138	28.89653368740821	25.896553805299057
47	20.479610517633333	19.99818938982437	34.91037479630636	24.611825296235942
48	20.763272778481902	18.469632043776528	29.79338926106987	30.973705916671694
49	20.177037439394855	21.98885468847446	33.058925302271305	24.775182569859375
50	19.11843402337699	23.706922566238156	30.372784517271214	26.801858893113646
51	20.01730138612268	19.384794898102882	30.992415555153197	29.605488160621242
52	20.865471663950753	18.610054921841996	35.861347496328484	24.66312591787877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	157.5
2	207.0
3	740.0
4	1273.0
5	885.0
6	497.0
7	530.5
8	564.0
9	622.5
10	681.0
11	669.0
12	657.0
13	658.5
14	603.5
15	547.0
16	545.0
17	543.0
18	513.5
19	484.0
20	607.0
21	730.0
22	867.0
23	1004.0
24	1041.5
25	1079.0
26	1464.5
27	1850.0
28	2487.0
29	3124.0
30	3587.0
31	4050.0
32	4717.5
33	5385.0
34	6720.5
35	8056.0
36	8390.0
37	8724.0
38	10334.0
39	13522.0
40	15100.0
41	21637.0
42	28174.0
43	39279.0
44	50384.0
45	62714.5
46	75045.0
47	74341.5
48	73638.0
49	62798.5
50	51959.0
51	43430.0
52	34901.0
53	30122.0
54	25343.0
55	21893.5
56	18444.0
57	16940.5
58	15437.0
59	14596.0
60	13755.0
61	13572.5
62	13390.0
63	12005.5
64	8512.0
65	6403.0
66	5148.5
67	3894.0
68	3394.5
69	2895.0
70	2469.0
71	2043.0
72	1830.5
73	1618.0
74	1239.0
75	860.0
76	695.5
77	531.0
78	378.0
79	225.0
80	171.0
81	117.0
82	94.5
83	72.0
84	53.5
85	35.0
86	24.0
87	13.0
88	8.5
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	497070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.145039596097956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34866439865236	20.905182342338843
2	12.270430281624153	7.397852134014389
3	4.542831916308537	4.1083154398661526
4	2.1290760696063478	2.5672432968555188
5	1.2975863617601688	1.955789612730849
6	0.9034867688255647	1.634138665247835
7	0.6069523560449324	1.2807621964143603
8	0.5301115924545251	1.2784187955913762
9	0.5545361717851711	1.5044863370339303
>10	7.497325953992926	43.966125876289034
>50	0.23120662707171175	4.665276594356843
>100	0.07840920396345005	4.543457784050812
>500	0.0053613130915179524	1.1907132138370602
>1k	0.004020984818638464	3.002237711373015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	4212	0.8473655621944595	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT	3788	0.7620657050314844	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	2436	0.49007182087029993	No Hit
TGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTTG	1906	0.38344699941658117	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1466	0.29492827971915425	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1053	0.21184139054861487	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	927	0.18649284808980626	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	917	0.18448105900577383	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	869	0.17482447140241816	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	761	0.15309714929486792	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	711	0.14303820387470575	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.1263403544772366	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	566	0.11386726215623555	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	515	0.10360713782767014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7719234715432434	0.0
2	0.0	0.0	0.0	2.4642404490313234	0.0
3	0.0	0.0	0.0	3.7789446154465165	0.0
4	0.0	0.0	0.0	4.614842979861991	0.0
5	0.0	0.0	0.0	8.504637173838695	0.0
6	0.0	0.0	0.0	9.158267447240831	0.0
7	0.0	0.0	0.0	11.698352344740178	0.0
8	0.0	0.0	0.0	14.610618222785524	0.0
9	0.0	0.0	0.0	17.040658257388294	0.0
10	0.0	0.0	0.0	18.575452149596636	0.0
11	0.0	0.0	0.0	20.017703743939485	0.0
12	0.0	0.0	0.0	21.310881767155532	0.0
13	0.0	0.0	0.0	21.806184239644317	0.0
14	0.0	0.0	0.0	22.170921600579394	0.0
15	0.0	0.0	0.0	23.3685396423039	0.0
16	0.0	0.0	0.0	24.679019051642626	0.0
17	0.0	0.0	0.0	26.401512865391194	0.0
18	0.0	0.0	0.0	27.216086265515923	0.0
19	0.0	0.0	0.0	28.12340314241455	0.0
20	0.0	0.0	0.0	29.19407729293661	0.0
21	0.0	0.0	0.0	30.16999617760074	0.0
22	0.0	0.0	0.0	31.1972156839077	0.0
23	0.0	0.0	0.0	31.955660168587926	0.0
24	0.0	0.0	0.0	32.55980043052286	0.0
25	0.0	0.0	0.0	33.180638541855274	0.0
26	0.0	0.0	0.0	33.75259017844569	0.0
27	0.0	0.0	0.0	34.3179029110588	0.0
28	0.0	0.0	0.0	34.877984992053435	0.0
29	0.0	0.0	0.0	35.60866678737401	0.0
30	0.0	0.0	0.0	36.28301848834168	0.0
31	0.0	0.0	0.0	36.842698211519505	0.0
32	0.0	0.0	0.0	37.39976260888808	0.0
33	0.0	0.0	0.0	37.92986903253063	0.0
34	0.0	0.0	0.0	38.661355543484824	0.0
35	0.0	0.0	0.0	39.1747641177299	0.0
36	0.0	0.0	0.0	39.72458607439596	0.0
37	0.0	0.0	0.0	40.27601746232925	0.0
38	0.0	0.0	0.0	40.77976944897097	0.0
39	0.0	0.0	0.0	41.35675055827147	0.0
40	0.0	0.0	0.0	41.94861890679381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCTG	55	1.8189894E-12	46.000004	1
TGACTAT	55	1.8189894E-12	46.000004	23
GTCATCG	20	6.30798E-4	46.0	43
ACCGGTT	20	6.30798E-4	46.0	13
TTCACGG	25	3.414338E-5	46.0	1
CCGTGAC	20	6.30798E-4	46.0	31
CGCGCCA	25	3.414338E-5	46.0	12
TAAGTGG	20	6.30798E-4	46.0	1
TATGACT	20	6.30798E-4	46.0	27
TGCGCTC	25	3.414338E-5	46.0	23
TACGCGC	20	6.30798E-4	46.0	34
ATGACTA	30	1.8592564E-6	46.0	13
ATGACGG	60	0.0	46.0	1
AAGATTC	20	6.30798E-4	46.0	26
CCAACGG	20	6.30798E-4	46.0	1
TCGCGCC	25	3.414338E-5	46.0	11
TCAACGG	20	6.30798E-4	46.0	1
CCACCGG	25	3.414338E-5	46.0	1
GACTATA	20	6.30798E-4	46.0	8
TCGATGG	35	1.0179974E-7	45.999996	1
>>END_MODULE