FastQCFastQC Report
Thu 26 May 2016
SRR1544756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544756_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1080369
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT58640.5427775139790201No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT50350.46604447184249087Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT34080.31544777756488757No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.2052076651588485No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16750.1550396207221792No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC15350.14208108525883287No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT15080.13958193913375894No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC13300.12310608690179003No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC12200.11292438046630364No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11360.10514925918829585No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT11090.10265011306322191No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT10970.10153938145207794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG2250.046.0000041
GGTACGA206.3112867E-446.023
GTACCGG301.8612991E-646.01
CGACGGT501.6370905E-1146.027
ACGTTCA206.3112867E-446.011
CCGATAG253.417018E-546.011
CGGCATA206.3112867E-446.033
AATATCG405.6115823E-946.023
CGTTAAT405.6115823E-946.021
ATCCCGC206.3112867E-446.023
TCTCGCA206.3112867E-446.029
CGTAAAC206.3112867E-446.023
TATACGA206.3112867E-446.033
TCCCACG206.3112867E-446.022
AATACGA253.417018E-546.019
AGTACGG1200.046.01
AATCGCC206.3112867E-446.028
GATCGCG351.019489E-745.9999968
AATACGG351.019489E-745.9999961
AATGCGG2000.044.8500021
Produced by FastQC (version 0.11.5)