FastQCFastQC Report
Thu 26 May 2016
SRR1544755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544755_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences785300
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT69630.886667515599134No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT58690.7473576976951484Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT38300.48771170253406343No Hit
TGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTTG27560.3509486820323443Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA27020.34407232904622437No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC14800.18846300776773206No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT14770.18808098815739208No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC13980.1780211384184388No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC13010.16566917101744555No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC11220.14287533426715904No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10970.13969183751432573No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1309053864765058No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT10180.12963198777537246No Hit
CTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTTGAAA9420.1199541576467592Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT8620.10976696803769259No Hit
AGGCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT8300.10569209219406597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG2150.046.02
CGACGGT600.046.027
GCGAATC206.310228E-446.037
CTATCGG253.416161E-546.01
CGGTTGA206.310228E-446.020
CGTTGAA206.310228E-446.029
TAAACGG253.416161E-546.01
GTTACGG501.6370905E-1146.01
CCCGTTG206.310228E-446.027
TTGGTCA206.310228E-446.023
GCCTATA206.310228E-446.045
GCGATAG850.046.08
CCGAAGC253.416161E-546.027
GGCGATA1500.046.07
CACAACG301.8606443E-646.011
TGGGACG206.310228E-446.05
GGTTAAC253.416161E-546.07
CCGTTGA206.310228E-446.028
TCAACGG301.8606443E-646.01
ACGGTCT600.046.029
Produced by FastQC (version 0.11.5)