##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544755_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	785300
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89760855723927	34.0	33.0	34.0	31.0	34.0
2	33.23880300522094	34.0	33.0	34.0	31.0	34.0
3	33.329418056793585	34.0	34.0	34.0	31.0	34.0
4	36.57139182478034	37.0	37.0	37.0	35.0	37.0
5	36.57082006876353	37.0	37.0	37.0	35.0	37.0
6	36.50653762893162	37.0	37.0	37.0	35.0	37.0
7	36.371924105437415	37.0	37.0	37.0	35.0	37.0
8	36.42862472940278	37.0	37.0	37.0	35.0	37.0
9	38.28781357443015	39.0	39.0	39.0	37.0	39.0
10	38.130487711702536	39.0	39.0	39.0	35.0	39.0
11	37.46400993250987	39.0	35.0	39.0	35.0	39.0
12	37.40813829109894	39.0	35.0	39.0	35.0	39.0
13	37.394612250095506	39.0	35.0	39.0	35.0	39.0
14	38.64296065198013	41.0	35.0	41.0	35.0	41.0
15	38.70963453457277	41.0	35.0	41.0	35.0	41.0
16	38.7047179421877	41.0	35.0	41.0	35.0	41.0
17	38.775588946899276	41.0	36.0	41.0	35.0	41.0
18	38.78122500955049	41.0	36.0	41.0	35.0	41.0
19	38.71115497262193	41.0	35.0	41.0	35.0	41.0
20	38.6384260792054	41.0	35.0	41.0	35.0	41.0
21	38.58645613141475	41.0	35.0	41.0	35.0	41.0
22	38.56260664714122	41.0	35.0	41.0	35.0	41.0
23	38.519835731567554	40.0	35.0	41.0	35.0	41.0
24	38.46234178021138	40.0	35.0	41.0	35.0	41.0
25	38.421656691710176	40.0	35.0	41.0	35.0	41.0
26	38.33510250859544	40.0	35.0	41.0	35.0	41.0
27	38.317338596714634	40.0	35.0	41.0	35.0	41.0
28	38.29060486438304	40.0	35.0	41.0	35.0	41.0
29	38.335950592130395	40.0	36.0	41.0	35.0	41.0
30	38.30794346109767	40.0	35.0	41.0	35.0	41.0
31	38.167949828091174	40.0	35.0	41.0	35.0	41.0
32	38.20430154081243	40.0	35.0	41.0	35.0	41.0
33	38.109511014898764	40.0	35.0	41.0	35.0	41.0
34	38.00507322042532	40.0	35.0	41.0	35.0	41.0
35	37.94660257226538	40.0	35.0	41.0	35.0	41.0
36	37.95433592257736	40.0	35.0	41.0	35.0	41.0
37	37.940105692092196	40.0	35.0	41.0	35.0	41.0
38	37.879631987775376	40.0	35.0	41.0	35.0	41.0
39	37.87534572774736	40.0	35.0	41.0	35.0	41.0
40	37.85144658092449	40.0	35.0	41.0	35.0	41.0
41	37.80060868457914	40.0	35.0	41.0	35.0	41.0
42	37.71196994779066	40.0	35.0	41.0	35.0	41.0
43	37.59492295937858	39.0	35.0	41.0	35.0	41.0
44	37.49941805679358	39.0	35.0	41.0	35.0	41.0
45	37.49362409270343	39.0	35.0	41.0	35.0	41.0
46	37.44026996052464	39.0	35.0	41.0	35.0	41.0
47	37.3651687253279	39.0	35.0	41.0	35.0	41.0
48	37.31901184260792	39.0	35.0	41.0	35.0	41.0
49	37.28981535718834	39.0	35.0	41.0	35.0	41.0
50	37.21137017700242	38.0	35.0	41.0	35.0	41.0
51	37.139452438558514	38.0	35.0	41.0	34.0	41.0
52	36.73859416783395	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	7.0
15	11.0
16	20.0
17	29.0
18	67.0
19	114.0
20	167.0
21	317.0
22	460.0
23	716.0
24	1215.0
25	1827.0
26	2641.0
27	3294.0
28	3347.0
29	3628.0
30	4389.0
31	5923.0
32	7384.0
33	10847.0
34	38043.0
35	163698.0
36	28966.0
37	45254.0
38	81826.0
39	379301.0
40	1805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.006749013116007	21.76735005730294	23.35489621800586	23.871004711575196
2	26.86667515599134	23.835476887813574	27.00420221571374	22.293645740481345
3	27.269578505029923	24.47001146058831	25.97223990831529	22.288170126066472
4	26.22080733477652	26.175219661275946	23.937603463644468	23.66636954030307
5	23.961798038966002	34.44288806825417	20.9579778428626	20.63733604991723
6	83.83012861326881	2.9845918757162866	9.013625366102126	4.171654144912773
7	84.78925251496243	2.676811409652362	6.797784286260028	5.736151789125175
8	73.99032216987139	4.641792945371196	12.942697058449001	8.425187826308417
9	50.55138163759073	18.473704316821596	15.309181204635172	15.6657328409525
10	40.81777664586782	19.50286514707755	25.84986629313638	13.829491913918249
11	38.05577486310964	17.392970839169745	24.11435120336177	20.436903094358843
12	20.963962816757927	31.906914554947154	28.237616197631475	18.89150643066344
13	13.895199286896728	34.02037437921813	30.13205144530753	21.952374888577612
14	13.629058958359863	24.034254425060485	41.830892652489496	20.50579396409016
15	16.109002928817013	17.559531389277982	45.06341525531644	21.268050426588562
16	15.766076658601808	22.800713103272635	27.171781484782887	34.26142875334267
17	18.322424551126957	23.16503247166688	32.62562078186681	25.886922195339363
18	23.70660893925888	27.247421367630203	26.98357315675538	22.062396536355532
19	26.42378708773717	22.09588692219534	25.459314911498794	26.0210110785687
20	20.20781866802496	30.782503501846424	26.959760601044184	22.049917229084425
21	20.32140583216605	24.301795492168598	26.157392079460077	29.21940659620527
22	18.319368394244236	28.602062905895835	23.71488603081625	29.363682669043676
23	18.77027887431555	21.89952884248058	36.95148350948682	22.37870877371705
24	21.06379727492678	21.71526805042659	31.720361645231122	25.500573029415506
25	17.320387113205143	29.733095632242456	27.672991213548965	25.273526041003436
26	19.596841971221192	27.35120336177257	27.47013880045842	25.581815866547814
27	22.576722271743282	24.442506048643832	32.99961798038966	19.981153699223228
28	17.920412581179168	24.258117916719723	31.516363173309564	26.305106328791545
29	23.553164395772317	26.189481726728637	27.780720743664844	22.476633133834202
30	29.063797274926777	23.60066216732459	23.851139691837513	23.484400865911116
31	28.249331465681905	23.086973131287404	26.852413090538647	21.811282312492043
32	27.115369922322678	27.142620654526933	23.17929453711957	22.562714886030815
33	19.964981535718834	23.26830510632879	31.35362281930472	25.413090538647648
34	20.62625748121737	23.26906914554947	33.22042531516618	22.88424805806698
35	25.274544759964346	23.061759837004967	27.406978224882213	24.256717178148477
36	21.394880937221444	29.591621036546545	29.1203361772571	19.893161848974913
37	20.44785432318859	26.08289825544378	30.747485037565266	22.721762383802368
38	18.01133324844009	27.926397555074495	27.620272507322042	26.44199668916338
39	26.297975296065196	23.089265248949445	26.9904495097415	23.622309945243856
40	17.37870877371705	20.444543486565646	32.28447726983318	29.892270469884117
41	21.942824398319114	24.369667642939007	24.835986247294027	28.85152171144785
42	22.47013880045842	21.197886158156116	28.63007767732077	27.70189736406469
43	23.65605501082389	25.46389914682287	28.565771042913536	22.314274799439705
44	19.037947281293775	28.014771424933148	27.91226282949191	25.035018464281166
45	21.756398828473195	24.481726728638737	28.012351967400996	25.749522475487076
46	23.388131924105437	21.173309563224247	29.542849866293135	25.89570864637718
47	20.84286260028015	18.970711829873935	34.62867693874953	25.557748631096395
48	20.293263720871003	18.864510378199416	30.068508850120974	30.773717050808607
49	19.8968547052082	22.093976824143642	32.8491022539157	25.160066216732456
50	19.170635425951865	23.340506812683053	30.367248185406854	27.12160957595823
51	19.705335540557748	19.490385839806443	31.153189863746338	29.65108875588947
52	20.93047243091812	18.424678466827967	35.63427989303451	25.01056920921941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	254.5
2	316.0
3	1173.0
4	2030.0
5	1399.5
6	769.0
7	851.5
8	934.0
9	994.5
10	1055.0
11	1009.5
12	964.0
13	936.5
14	918.5
15	928.0
16	902.0
17	876.0
18	883.5
19	891.0
20	1233.5
21	1576.0
22	1469.0
23	1362.0
24	1494.0
25	1626.0
26	1869.0
27	2112.0
28	3276.0
29	4440.0
30	4906.0
31	5372.0
32	5556.5
33	5741.0
34	7086.0
35	8431.0
36	9857.5
37	11284.0
38	12974.5
39	17802.0
40	20939.0
41	31380.5
42	41822.0
43	57851.0
44	73880.0
45	92918.5
46	111957.0
47	110673.5
48	109390.0
49	93878.0
50	78366.0
51	68307.0
52	58248.0
53	50756.0
54	43264.0
55	39310.0
56	35356.0
57	32115.5
58	28875.0
59	27800.5
60	26726.0
61	25860.5
62	24995.0
63	23648.0
64	17811.0
65	13321.0
66	11516.0
67	9711.0
68	7993.0
69	6275.0
70	5339.5
71	4404.0
72	3788.5
73	3173.0
74	2834.0
75	2495.0
76	2233.5
77	1972.0
78	1348.0
79	724.0
80	539.5
81	355.0
82	260.0
83	165.0
84	92.0
85	19.0
86	46.0
87	73.0
88	39.5
89	9.0
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	785300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.94211433130738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44057457815123	17.754932106067685
2	12.931239372471733	6.7092738049233045
3	5.147389728058479	4.0060251849927075
4	2.4773644511764896	2.5707228733094825
5	1.3466387765458159	1.7467328552061723
6	0.9121909714873301	1.4198497484586399
7	0.5847879500489194	1.0619445101817957
8	0.44143790829529367	0.9161466149735753
9	0.3844382908427377	0.897582788493723
>10	6.773734625538053	44.19905471323196
>50	0.41140703172151344	7.185393295135474
>100	0.13401522470260924	5.994516866103517
>500	0.008375951543913077	1.4424214340173176
>1k	0.005419733351943756	2.4552479686343514
>5k	9.854060639897739E-4	1.6401552362702898
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	6963	0.886667515599134	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	5869	0.7473576976951484	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	3830	0.48771170253406343	No Hit
TGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTTG	2756	0.3509486820323443	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2702	0.34407232904622437	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1480	0.18846300776773206	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1477	0.18808098815739208	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1398	0.1780211384184388	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1301	0.16566917101744555	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1122	0.14287533426715904	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1097	0.13969183751432573	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.1309053864765058	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1018	0.12963198777537246	No Hit
CTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTTGAAA	942	0.1199541576467592	Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	862	0.10976696803769259	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	830	0.10569209219406597	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7649305997707883	0.0
2	0.0	0.0	0.0	2.4901311600662166	0.0
3	0.0	0.0	0.0	3.7271106583471285	0.0
4	0.0	0.0	0.0	4.505666624220043	0.0
5	0.0	0.0	0.0	8.305233668661659	0.0
6	0.0	0.0	0.0	8.94919139182478	0.0
7	0.0	0.0	0.0	11.453839297083917	0.0
8	0.0	0.0	0.0	14.217878517763912	0.0
9	0.0	0.0	0.0	16.420348911244112	0.0
10	0.0	0.0	0.0	17.841079842098562	0.0
11	0.0	0.0	0.0	19.178657837769006	0.0
12	0.0	0.0	0.0	20.40850630332357	0.0
13	1.2733987011333248E-4	0.0	0.0	20.923978097542342	0.0
14	1.2733987011333248E-4	0.0	0.0	21.30714376671336	0.0
15	1.2733987011333248E-4	0.0	0.0	22.464917865783775	0.0
16	1.2733987011333248E-4	0.0	0.0	23.645231121864256	0.0
17	1.2733987011333248E-4	0.0	0.0	25.241054374124538	0.0
18	1.2733987011333248E-4	0.0	0.0	25.995925124156372	0.0
19	1.2733987011333248E-4	0.0	0.0	26.82987393352859	0.0
20	1.2733987011333248E-4	0.0	0.0	27.864001018718962	0.0
21	2.5467974022666496E-4	0.0	0.0	28.721125684451803	0.0
22	2.5467974022666496E-4	0.0	0.0	29.672481854068508	0.0
23	2.5467974022666496E-4	0.0	0.0	30.362918629822996	0.0
24	3.8201961033999744E-4	0.0	0.0	30.958614542213166	0.0
25	3.8201961033999744E-4	0.0	0.0	31.518018591621036	0.0
26	3.8201961033999744E-4	0.0	0.0	32.00305615688272	0.0
27	3.8201961033999744E-4	0.0	0.0	32.49828091175347	0.0
28	5.093594804533299E-4	0.0	0.0	33.01820960142621	0.0
29	5.093594804533299E-4	0.0	0.0	33.65720106965491	0.0
30	5.093594804533299E-4	0.0	0.0	34.269451165159815	0.0
31	5.093594804533299E-4	0.0	0.0	34.81013625366102	0.0
32	5.093594804533299E-4	0.0	0.0	35.30981790398574	0.0
33	5.093594804533299E-4	0.0	0.0	35.84375397937094	0.0
34	5.093594804533299E-4	0.0	0.0	36.530879918502485	0.0
35	7.640392206799949E-4	0.0	0.0	37.01680886285496	0.0
36	7.640392206799949E-4	0.0	0.0	37.54692474213676	0.0
37	8.913790907933274E-4	0.0	0.0	38.009423150388386	0.0
38	8.913790907933274E-4	0.0	0.0	38.52655036291863	0.0
39	8.913790907933274E-4	0.0	0.0	39.063669935056666	0.0
40	0.0010187189609066599	0.0	0.0	39.62396536355533	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	215	0.0	46.0	2
CGACGGT	60	0.0	46.0	27
GCGAATC	20	6.310228E-4	46.0	37
CTATCGG	25	3.416161E-5	46.0	1
CGGTTGA	20	6.310228E-4	46.0	20
CGTTGAA	20	6.310228E-4	46.0	29
TAAACGG	25	3.416161E-5	46.0	1
GTTACGG	50	1.6370905E-11	46.0	1
CCCGTTG	20	6.310228E-4	46.0	27
TTGGTCA	20	6.310228E-4	46.0	23
GCCTATA	20	6.310228E-4	46.0	45
GCGATAG	85	0.0	46.0	8
CCGAAGC	25	3.416161E-5	46.0	27
GGCGATA	150	0.0	46.0	7
CACAACG	30	1.8606443E-6	46.0	11
TGGGACG	20	6.310228E-4	46.0	5
GGTTAAC	25	3.416161E-5	46.0	7
CCGTTGA	20	6.310228E-4	46.0	28
TCAACGG	30	1.8606443E-6	46.0	1
ACGGTCT	60	0.0	46.0	29
>>END_MODULE