FastQCFastQC Report
Thu 26 May 2016
SRR1544754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544754_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences552058
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT22480.4072035909270403No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT21720.39343692148288767Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT15010.27189172152201396No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.1458180118755638No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC7800.1412895021899873No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT6280.11375616330168206No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC5930.10741624974187494No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC5530.10017063424495254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA551.8189894E-1246.0000044
TTGACGG551.8189894E-1246.0000041
TAGTCGG551.8189894E-1246.0000041
ACAACGA301.8596329E-646.012
CTTAACA206.30859E-446.014
CGGGTGG650.046.05
CGGGTGC206.30859E-446.05
AGGTAGT253.4148325E-546.09
CTGTCGG253.4148325E-546.01
TCGCAAA206.30859E-446.010
ACCGGGT301.8596329E-646.03
CGTATAA650.046.025
TACCACC253.4148325E-546.020
TTTACGG206.30859E-446.01
CGAAAGC206.30859E-446.034
GACACGG1000.046.01
GGCCGAT253.4148325E-546.07
GGCCGAA206.30859E-446.031
TGAACTT206.30859E-446.019
GAATCAG253.4148325E-546.08
Produced by FastQC (version 0.11.5)