##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544754_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	552058
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91217408315793	34.0	33.0	34.0	31.0	34.0
2	33.23514195972162	34.0	33.0	34.0	31.0	34.0
3	33.28577250941024	34.0	34.0	34.0	31.0	34.0
4	36.56885508406725	37.0	37.0	37.0	35.0	37.0
5	36.56295353024501	37.0	37.0	37.0	35.0	37.0
6	36.67714261907263	37.0	37.0	37.0	35.0	37.0
7	36.64713127968438	37.0	37.0	37.0	35.0	37.0
8	36.5767528049589	37.0	37.0	37.0	35.0	37.0
9	38.3598643620779	39.0	39.0	39.0	37.0	39.0
10	38.045051425755986	39.0	39.0	39.0	35.0	39.0
11	36.949512551217445	39.0	35.0	39.0	35.0	39.0
12	36.794778809472916	39.0	35.0	39.0	35.0	39.0
13	36.776110481145096	39.0	35.0	39.0	35.0	39.0
14	37.90694999438465	40.0	35.0	41.0	35.0	41.0
15	38.00499585188513	40.0	35.0	41.0	35.0	41.0
16	38.01060758108749	40.0	35.0	41.0	35.0	41.0
17	38.13686424252524	40.0	35.0	41.0	35.0	41.0
18	38.107733245419865	40.0	36.0	41.0	35.0	41.0
19	37.96124863691858	39.0	35.0	41.0	35.0	41.0
20	37.86106713425038	39.0	35.0	41.0	35.0	41.0
21	37.819712783801705	39.0	35.0	41.0	35.0	41.0
22	37.80540088179141	39.0	35.0	41.0	35.0	41.0
23	37.778657314992266	39.0	35.0	41.0	35.0	41.0
24	37.738650649026006	39.0	35.0	41.0	35.0	41.0
25	37.70108394407834	39.0	35.0	41.0	35.0	41.0
26	37.63889301486438	39.0	35.0	41.0	35.0	41.0
27	37.62889044267088	39.0	35.0	41.0	35.0	41.0
28	37.66324733995341	39.0	35.0	41.0	35.0	41.0
29	37.68778099402599	39.0	35.0	41.0	35.0	41.0
30	37.58129580587547	39.0	35.0	41.0	35.0	41.0
31	37.5000706447511	39.0	35.0	41.0	35.0	41.0
32	37.429376985751496	38.0	35.0	41.0	35.0	41.0
33	37.35121490857845	38.0	35.0	41.0	35.0	41.0
34	37.261744237018576	38.0	35.0	41.0	35.0	41.0
35	37.20318336116857	38.0	35.0	41.0	35.0	41.0
36	37.12082969543055	38.0	35.0	41.0	34.0	41.0
37	37.03837096826783	38.0	35.0	41.0	34.0	41.0
38	37.026810588742485	38.0	35.0	41.0	34.0	41.0
39	36.9214828876676	37.0	35.0	41.0	34.0	41.0
40	36.8604403885099	37.0	35.0	41.0	34.0	41.0
41	36.834414137644956	37.0	35.0	41.0	34.0	41.0
42	36.745546663575205	37.0	35.0	41.0	34.0	41.0
43	36.74356861054454	37.0	35.0	41.0	34.0	41.0
44	36.708871169333655	36.0	35.0	41.0	34.0	41.0
45	36.72717178267501	36.0	35.0	41.0	34.0	41.0
46	36.716154824311936	36.0	35.0	41.0	34.0	41.0
47	36.655566987526676	36.0	35.0	41.0	34.0	41.0
48	36.662830717062334	36.0	35.0	41.0	34.0	41.0
49	36.556939669382565	36.0	35.0	41.0	34.0	41.0
50	36.47300827087009	36.0	35.0	40.0	34.0	41.0
51	36.40257364262451	36.0	35.0	40.0	34.0	41.0
52	36.161771045795916	35.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	3.0
16	7.0
17	21.0
18	37.0
19	78.0
20	156.0
21	249.0
22	401.0
23	687.0
24	1270.0
25	2024.0
26	2694.0
27	3178.0
28	3150.0
29	3500.0
30	4004.0
31	4984.0
32	6488.0
33	9919.0
34	34520.0
35	168323.0
36	17849.0
37	25416.0
38	44308.0
39	216899.0
40	1890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.262399965221043	22.138072448909355	26.42747682308743	21.17205076278217
2	24.994837498958443	23.946759217328616	28.27873158255111	22.779671701161835
3	25.878621449195556	24.783627807223155	27.28553883831047	22.052211905270823
4	25.05859891533136	28.26587061504407	24.758811574146193	21.916718895478375
5	24.243467171927588	34.26976875618142	21.112998996482254	20.37376507540874
6	88.05397258983658	2.572374641794884	6.870654894956689	2.502997873411852
7	88.96710128283623	1.8606740596096787	4.770150962398878	4.402073695155219
8	80.69840487774836	3.484597632857417	10.028837549677752	5.788159939716479
9	58.838926344695665	15.456709258809761	12.485101203134453	13.219263193360117
10	52.6691036086788	17.212321893714066	20.258378648620255	9.860195848986882
11	47.675968829362134	16.58358360896862	21.432168359121686	14.308279202547558
12	19.76748819870376	42.40170416876487	23.269837589528635	14.560970043002728
13	11.603490937546418	45.813664506265646	26.89137735527789	15.69146720091005
14	10.31866216955465	21.363878433063192	53.57263186114503	14.744827536237134
15	12.167562103981828	15.593651391701597	53.60469370971891	18.63409279459767
16	12.951718841136255	18.16602603349648	27.00984316865257	41.87241195671469
17	19.857696111640443	20.44984403812643	36.532936756645135	23.159523093587993
18	26.829427342779198	26.469682533357002	25.404577055309407	21.29631306855439
19	30.871575088124796	21.160276637599672	24.343275525397694	23.624872748877834
20	18.156787873737905	32.01040470385358	26.60155273540099	23.231254687007524
21	19.84809567110702	23.81507015567205	25.305312123001567	31.031522050219362
22	16.307887939310724	32.514518402051955	21.818540805495072	29.35905285314224
23	17.93688344340631	21.638124979621708	39.71575450405573	20.70923707291625
24	15.420118900550307	21.801875889852155	38.35158624637266	24.426418963224876
25	12.676747733028051	35.4297917972387	29.343474779823858	22.549985689909395
26	15.14641577515406	33.54611290842629	28.244133768553304	23.063337547866347
27	15.756496599994929	27.381905524419537	41.181542519083145	15.680055356502395
28	13.758699267105992	23.565277561415648	37.61054092142492	25.06548225005344
29	16.109358074695052	32.22088983403918	30.23486662633274	21.434885464933032
30	29.42806734075043	25.294987120918456	23.186331870926605	22.09061366740451
31	29.17374623680845	28.446105300530018	23.50314640852954	18.877002054131996
32	24.462828181096913	32.22994685341033	23.02384894340812	20.28337602208464
33	17.774038235112975	28.17040963087212	28.627064547565656	25.42848758644925
34	16.14775983682874	28.32383553901945	32.53462498505591	22.993779639095894
35	30.27888374047654	21.613308746544746	27.587862144919555	20.519945368059155
36	22.91009278010644	30.414376750268996	27.717377521927045	18.958152947697524
37	19.914574193291283	31.521144517423892	24.4383742287948	24.12590706049002
38	19.261019675468884	29.870049886062695	26.02443946107112	24.844490977397303
39	26.868372526075156	24.810980005724037	22.778041437675025	25.54260603052578
40	17.02556615428089	20.958486246010384	29.6525002807676	32.36344731894113
41	22.360150563890027	25.319441073220567	23.865608323763084	28.454800039126322
42	22.85375811961787	23.501516145042732	27.058751073256797	26.585974662082606
43	22.573171659499543	28.990975585898582	27.924058703976755	20.511794050625117
44	15.727695278394663	37.60275188476573	23.561654753667188	23.107898083172422
45	23.746780229613552	29.87113672838723	25.59169507551743	20.79038796648178
46	22.55560104191951	26.161019313188106	26.224961869948448	25.058417774943937
47	23.387397700966204	19.49414735408236	31.906792402247593	25.211662542703845
48	21.70514692296824	17.722956645859675	30.50929431327868	30.062602117893412
49	19.957685605497975	27.33299761981531	29.414300671306275	23.29501610338044
50	18.855989769190916	30.821036920033762	24.68707998072666	25.635893330048653
51	21.05358494940749	22.70015107108311	30.492267116860912	25.75399686264849
52	23.895858768462734	20.199145741932913	31.810063435363677	24.09493205424068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	243.0
1	234.5
2	226.0
3	865.0
4	1504.0
5	1102.0
6	700.0
7	829.5
8	959.0
9	982.5
10	1006.0
11	969.5
12	933.0
13	904.0
14	850.5
15	826.0
16	763.5
17	701.0
18	767.5
19	834.0
20	732.5
21	631.0
22	729.5
23	828.0
24	1121.5
25	1415.0
26	1491.5
27	1568.0
28	1730.0
29	1892.0
30	2425.5
31	2959.0
32	3480.5
33	4002.0
34	4844.0
35	5686.0
36	6607.5
37	7529.0
38	8684.5
39	13183.5
40	16527.0
41	26541.0
42	36555.0
43	55831.5
44	75108.0
45	83481.5
46	91855.0
47	85297.5
48	78740.0
49	66144.0
50	53548.0
51	46144.0
52	38740.0
53	32907.5
54	27075.0
55	23805.0
56	20535.0
57	18411.0
58	16287.0
59	15682.0
60	15077.0
61	13766.0
62	12455.0
63	10796.0
64	7362.0
65	5587.0
66	4458.0
67	3329.0
68	2875.5
69	2422.0
70	1878.0
71	1334.0
72	1268.5
73	1203.0
74	875.0
75	547.0
76	514.5
77	482.0
78	319.5
79	157.0
80	158.5
81	160.0
82	94.0
83	28.0
84	18.0
85	8.0
86	5.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	552058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.41842081413948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7650769934628	16.337879311637526
2	12.405448071083187	5.810320066331619
3	4.724850774504601	3.3194563116408475
4	2.2745413199231352	2.1306466315643293
5	1.4013998460972408	1.6409285662387745
6	0.8809243981139889	1.2377914956285558
7	0.5609029798014467	0.9194823411826538
8	0.47030319581645796	0.8811006519891563
9	0.35889654753696415	0.7564311341066196
>10	5.4266392865192845	35.12867350056243
>50	1.487374842302032	22.079991195067507
>100	0.23745401798581584	8.076380014648766
>500	0.00386732928315661	0.6084273900871358
>1k	0.002320397569893966	1.0724913893140609
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2248	0.4072035909270403	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	2172	0.39343692148288767	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1501	0.27189172152201396	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.1458180118755638	No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	780	0.1412895021899873	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	628	0.11375616330168206	No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	593	0.10741624974187494	No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	553	0.10017063424495254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4140869256491166	0.0
2	0.0	0.0	0.0	1.3355480764702259	0.0
3	0.0	0.0	0.0	2.1474192929003837	0.0
4	0.0	0.0	0.0	2.6625825547315682	0.0
5	0.0	0.0	0.0	5.19691771516761	0.0
6	0.0	0.0	0.0	5.678570005325527	0.0
7	0.0	0.0	0.0	7.893735803122135	0.0
8	0.0	0.0	0.0	10.268486282238461	0.0
9	0.0	0.0	0.0	12.6464972883284	0.0
10	0.0	0.0	0.0	13.636429505595427	0.0
11	0.0	0.0	0.0	14.645381463541874	0.0
12	0.0	0.0	0.0	15.551807962206869	0.0
13	0.0	0.0	0.0	15.864818551673919	0.0
14	0.0	0.0	0.0	16.11587912864228	0.0
15	0.0	0.0	0.0	17.02973238319162	0.0
16	0.0	0.0	0.0	18.00680363295161	0.0
17	0.0	0.0	0.0	19.7308978404443	0.0
18	0.0	0.0	0.0	20.31145278213521	0.0
19	0.0	0.0	0.0	21.158102952950596	0.0
20	0.0	0.0	0.0	22.003122860279174	0.0
21	1.811403874230606E-4	0.0	0.0	22.629144039213273	0.0
22	1.811403874230606E-4	0.0	0.0	23.377978400820204	0.0
23	1.811403874230606E-4	0.0	0.0	23.88028069514435	0.0
24	1.811403874230606E-4	0.0	0.0	24.32516148665539	0.0
25	1.811403874230606E-4	0.0	0.0	24.7627966626695	0.0
26	1.811403874230606E-4	0.0	0.0	25.1366704223107	0.0
27	1.811403874230606E-4	0.0	0.0	25.559089805781277	0.0
28	1.811403874230606E-4	0.0	0.0	25.965931115933472	0.0
29	1.811403874230606E-4	0.0	0.0	26.613145720196066	0.0
30	1.811403874230606E-4	0.0	0.0	27.190802415688207	0.0
31	1.811403874230606E-4	0.0	0.0	27.613221799158783	0.0
32	1.811403874230606E-4	0.0	0.0	28.02114995163552	0.0
33	1.811403874230606E-4	0.0	0.0	28.421651348227904	0.0
34	1.811403874230606E-4	0.0	0.0	29.07140191791442	0.0
35	1.811403874230606E-4	0.0	0.0	29.49671954758377	0.0
36	1.811403874230606E-4	0.0	0.0	29.92058805415373	0.0
37	5.434211622691819E-4	0.0	0.0	30.332139014378924	0.0
38	5.434211622691819E-4	0.0	0.0	30.691521543026276	0.0
39	5.434211622691819E-4	0.0	0.0	31.161580848389118	0.0
40	5.434211622691819E-4	0.0	0.0	31.59486865510508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	55	1.8189894E-12	46.000004	4
TTGACGG	55	1.8189894E-12	46.000004	1
TAGTCGG	55	1.8189894E-12	46.000004	1
ACAACGA	30	1.8596329E-6	46.0	12
CTTAACA	20	6.30859E-4	46.0	14
CGGGTGG	65	0.0	46.0	5
CGGGTGC	20	6.30859E-4	46.0	5
AGGTAGT	25	3.4148325E-5	46.0	9
CTGTCGG	25	3.4148325E-5	46.0	1
TCGCAAA	20	6.30859E-4	46.0	10
ACCGGGT	30	1.8596329E-6	46.0	3
CGTATAA	65	0.0	46.0	25
TACCACC	25	3.4148325E-5	46.0	20
TTTACGG	20	6.30859E-4	46.0	1
CGAAAGC	20	6.30859E-4	46.0	34
GACACGG	100	0.0	46.0	1
GGCCGAT	25	3.4148325E-5	46.0	7
GGCCGAA	20	6.30859E-4	46.0	31
TGAACTT	20	6.30859E-4	46.0	19
GAATCAG	25	3.4148325E-5	46.0	8
>>END_MODULE