FastQCFastQC Report
Thu 26 May 2016
SRR1544753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544753_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences423129
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT31530.7451628226852804No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT25490.6024167570646304Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT17220.4069680877462902No Hit
TGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTTG12300.2906914912473501Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC8200.1937943274982334No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT7100.16779752746798257No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC6330.149599767446807No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC6240.14747275653524103No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC5340.12620264741958126No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT4930.11651293104466959No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4630.109422894672783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGG1150.046.0000041
TTACGGG1150.046.0000042
CGGGTAG253.4134708E-546.05
GCGACAT206.306912E-446.040
GTCGATC206.306912E-446.08
CGTATAA253.4134708E-546.025
TACCACT206.306912E-446.09
TTTACGG206.306912E-446.01
CATGCAG253.4134708E-546.041
CCGGCCG206.306912E-446.018
ACCGCAA253.4134708E-546.032
CGGCAGT253.4134708E-546.011
GACCGAT453.092282E-1046.08
TCTTAGG453.092282E-1046.01
GATCCGG405.5970304E-946.01
GGATCGG253.4134708E-546.01
CGACCCG253.4134708E-546.031
CGACCCC206.306912E-446.019
ATGCTTC206.306912E-446.036
CCAGCGG206.306912E-446.01
Produced by FastQC (version 0.11.5)