FastQCFastQC Report
Thu 26 May 2016
SRR1544752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544752_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2295423
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92630.40354217937173237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54930.23930229853059765No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT31300.13635830955775907Illumina Single End Adapter 2 (95% over 21bp)
TTCAAAGGGACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCC26570.11575208578113924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCG351.020162E-746.00000410
TTTACGC253.418229E-546.023
CGATTCG800.046.010
TATGCGT405.6152203E-946.020
CCCAACG301.8622213E-646.023
GTGTCGA253.418229E-546.033
CGAATGT501.6370905E-1146.018
TATCGCC206.3127757E-446.034
TAGTCCG206.3127757E-446.015
CGAAGTA301.8622213E-646.039
TGCGTAG1650.044.6060641
TTACCGG1200.044.0833322
AATACGG4200.043.2619062
ACGCGAG700.042.7142871
TACGGGT2400.042.1666684
TAACGCG601.8189894E-1242.1666681
CGACGGT2900.042.03448528
ACGGGAT12850.041.883275
TGTGACG1050.041.6190451
TACGGGA10000.041.44
Produced by FastQC (version 0.11.5)