##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544752_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2295423
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70907627918689	33.0	31.0	34.0	31.0	34.0
2	33.12247982180191	34.0	33.0	34.0	31.0	34.0
3	33.16371840832822	34.0	33.0	34.0	31.0	34.0
4	36.48138970464267	37.0	37.0	37.0	35.0	37.0
5	36.4869324738839	37.0	37.0	37.0	35.0	37.0
6	36.54875855125613	37.0	37.0	37.0	35.0	37.0
7	36.63110154424697	37.0	37.0	37.0	35.0	37.0
8	36.62321802996659	37.0	37.0	37.0	35.0	37.0
9	38.28758882349789	39.0	39.0	39.0	37.0	39.0
10	38.222367729172355	39.0	39.0	39.0	37.0	39.0
11	38.15969082822643	39.0	39.0	39.0	35.0	39.0
12	37.77712473910037	39.0	37.0	39.0	35.0	39.0
13	37.69748320897717	39.0	37.0	39.0	35.0	39.0
14	38.99735473592449	41.0	38.0	41.0	35.0	41.0
15	39.020376636463084	41.0	38.0	41.0	35.0	41.0
16	39.05371994617114	41.0	38.0	41.0	35.0	41.0
17	39.01967349808728	41.0	38.0	41.0	35.0	41.0
18	39.01253407324053	41.0	38.0	41.0	35.0	41.0
19	38.9983449673546	41.0	38.0	41.0	35.0	41.0
20	38.917038820295865	41.0	38.0	41.0	35.0	41.0
21	38.86201279676992	41.0	37.0	41.0	35.0	41.0
22	38.8301524381345	41.0	37.0	41.0	35.0	41.0
23	38.73692866195032	41.0	37.0	41.0	35.0	41.0
24	38.69469505184883	41.0	37.0	41.0	35.0	41.0
25	38.70955767194108	40.0	37.0	41.0	35.0	41.0
26	38.654807850230654	40.0	37.0	41.0	35.0	41.0
27	38.63845356607475	40.0	37.0	41.0	35.0	41.0
28	38.57600712374146	40.0	36.0	41.0	35.0	41.0
29	38.5306072127011	40.0	36.0	41.0	35.0	41.0
30	38.47483753539108	40.0	36.0	41.0	35.0	41.0
31	38.40089473704847	40.0	36.0	41.0	35.0	41.0
32	38.298173364996345	40.0	36.0	41.0	35.0	41.0
33	38.18768261884629	40.0	36.0	41.0	35.0	41.0
34	38.05438823258284	40.0	36.0	41.0	35.0	41.0
35	37.95301345329379	40.0	36.0	41.0	34.0	41.0
36	37.887342768631314	40.0	36.0	41.0	34.0	41.0
37	37.86146082878842	40.0	36.0	41.0	34.0	41.0
38	37.729802306590116	40.0	35.0	41.0	34.0	41.0
39	37.72995478393307	40.0	35.0	41.0	34.0	41.0
40	37.69716605610382	40.0	35.0	41.0	34.0	41.0
41	37.64376849060064	40.0	35.0	41.0	34.0	41.0
42	37.62484823058757	40.0	35.0	41.0	34.0	41.0
43	37.57565032675895	40.0	35.0	41.0	34.0	41.0
44	37.48411077174011	40.0	35.0	41.0	34.0	41.0
45	37.40528216367964	40.0	35.0	41.0	34.0	41.0
46	37.3425856585039	40.0	35.0	41.0	34.0	41.0
47	37.28391847602816	40.0	35.0	41.0	33.0	41.0
48	37.22405064338904	40.0	35.0	41.0	33.0	41.0
49	37.1689440246961	40.0	35.0	41.0	33.0	41.0
50	37.08149478331445	40.0	35.0	41.0	33.0	41.0
51	37.02999664985495	40.0	35.0	41.0	33.0	41.0
52	36.829867087678394	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	10.0
12	20.0
13	13.0
14	33.0
15	36.0
16	60.0
17	108.0
18	199.0
19	465.0
20	833.0
21	1422.0
22	2538.0
23	4287.0
24	7130.0
25	11307.0
26	15320.0
27	17133.0
28	17017.0
29	17215.0
30	18588.0
31	21783.0
32	27939.0
33	38693.0
34	88560.0
35	281805.0
36	88257.0
37	121920.0
38	253199.0
39	1255745.0
40	3780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.41156597280763	23.60906900383938	28.190577510114696	13.7887875132383
2	31.475157302161737	25.184639171080885	29.357682658054745	13.98252086870263
3	28.539707060528713	25.06823361097279	32.03888782154749	14.353171506951007
4	25.324831196690106	29.00345600789048	30.801076751431	14.870636043988405
5	23.337310813736728	33.910307599078685	28.11677847612401	14.635603111060574
6	22.325253341105324	42.47713820067151	24.83912551194268	10.358482946280489
7	87.83352785085799	4.067703425468857	6.124361392213984	1.974407331459169
8	87.56651824086454	3.498265896961039	6.638297167885831	2.296918694288591
9	81.82313238126481	6.4687859274739345	8.03468467467652	3.673397016584743
10	33.0489413062429	40.54647008416314	14.859396285564797	11.545192324029166
11	33.263890794855676	24.45828067419382	26.896306258149373	15.381522272801135
12	31.759854283938083	23.376693533174496	28.80327503906687	16.06017714382055
13	23.22996676429573	31.13752889990211	29.570541028821268	16.061963306980893
14	17.298075343847298	34.27455418892291	31.367987512541262	17.059382954688527
15	16.840164100472986	27.385932788858526	39.953812434570885	15.820090676097609
16	20.36809773187774	25.28348805427148	37.50258666921086	16.84582754463992
17	19.81360298297961	24.74685493697676	31.077801346418504	24.36174073362513
18	20.508551147217744	24.908742310240857	35.952763390451345	18.62994315209005
19	24.055914748610604	26.623110424527418	30.289014268829757	19.031960558032225
20	24.696885933442335	27.06634027802283	30.99010509174126	17.246668696793577
21	21.17435435647373	28.165876180555827	33.23940729007246	17.42036217289798
22	20.853803416625173	24.414236504557113	30.9059811633847	23.825978915433016
23	17.174830085783753	29.466725740745826	31.43782213561509	21.920622037855335
24	17.167206218635954	26.814752662145498	37.700981474874126	18.31705964434442
25	18.334049976845225	28.88983860491073	33.62264820035349	19.15346321789056
26	17.96518550175719	32.628975138787055	29.944023389153113	19.46181597030264
27	17.142984103583522	33.67767073868303	30.203714086684673	18.975631071048777
28	15.949173638148611	29.863558917027493	35.13888289870756	19.04838454611634
29	17.91255903595982	26.428462205005353	34.79293359001805	20.86604516901678
30	18.485481760878063	31.346509989662035	32.14766951450778	18.02033873495212
31	22.73123515796435	29.492559759138075	29.891353358400607	17.88485172449697
32	23.295749846542446	27.976499320604525	31.223482556374144	17.50426827647889
33	23.06934277473041	28.429705548824767	29.223284771477847	19.27766690496697
34	18.220737528551382	28.55347358634988	31.104332404092837	22.121456481005897
35	18.828817172259754	27.793744333833025	33.15637248559416	20.221066008313066
36	23.501463564667603	28.245295093758315	30.020915534958043	18.232325806616036
37	18.7080115516835	33.15828934361989	30.307877894401162	17.825821210295445
38	19.02555650962807	31.762119661604853	29.423465740301463	19.78885808846561
39	18.898869620109235	30.86354889708781	30.421103212784743	19.816478270018205
40	21.549622879965916	27.120535082204896	30.72832327636344	20.60151876146575
41	17.267362050480457	27.84928965162412	30.825821645944995	24.057526651950425
42	18.619923212410086	27.37085931438345	30.69146732432323	23.317750148883235
43	19.4230431602367	27.001384929923596	31.51057561068265	22.064996299157063
44	17.78530580202429	28.90669824254615	32.10157779197995	21.206418163449612
45	17.433431659437062	33.61363025464152	29.365393655112804	19.587544430808613
46	19.27714412550541	31.689671141223208	29.92293795087006	19.110246782401326
47	18.36358701642355	29.607353415906353	30.79571826194998	21.23334130572012
48	18.567296746612715	28.64779171420692	33.10984511351502	19.675066425665335
49	20.600386072632364	27.388111036615037	32.90177888781283	19.109724002939764
50	18.738332760454174	31.955940146979444	29.914529914529915	19.391197178036467
51	18.24064671304592	30.57867765549095	29.43819069513549	21.74248493632764
52	17.974682661975592	28.460941621653173	33.91575321846997	19.648622497901258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6547.0
1	6419.0
2	6291.0
3	11727.0
4	17163.0
5	11805.0
6	6447.0
7	6806.0
8	7165.0
9	7352.5
10	7540.0
11	7962.0
12	8384.0
13	8565.5
14	8116.0
15	7485.0
16	7048.5
17	6612.0
18	8305.5
19	9999.0
20	11872.5
21	13746.0
22	15188.5
23	16631.0
24	19006.5
25	21382.0
26	24252.0
27	27122.0
28	37658.0
29	48194.0
30	53476.0
31	58758.0
32	67027.0
33	75296.0
34	84386.0
35	93476.0
36	104730.0
37	115984.0
38	131101.5
39	155007.5
40	163796.0
41	189483.5
42	215171.0
43	221076.5
44	226982.0
45	222201.5
46	217421.0
47	203276.5
48	189132.0
49	171880.0
50	154628.0
51	133591.0
52	112554.0
53	98622.5
54	84691.0
55	75095.0
56	65499.0
57	56502.5
58	47506.0
59	39869.0
60	32232.0
61	27990.5
62	23749.0
63	20282.5
64	14570.5
65	12325.0
66	10462.0
67	8599.0
68	7092.5
69	5586.0
70	4637.5
71	3689.0
72	2960.5
73	2232.0
74	1875.5
75	1519.0
76	1308.5
77	1098.0
78	794.0
79	490.0
80	363.0
81	236.0
82	198.0
83	160.0
84	120.5
85	81.0
86	49.0
87	17.0
88	13.5
89	6.5
90	3.0
91	4.0
92	5.0
93	4.0
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2295423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.06548019971817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6811647510818	12.158542538001134
2	9.591204436363297	3.081746099276873
3	3.2280204487423774	1.555790958106681
4	1.6540430885809931	1.0629198595631453
5	1.0191484925352288	0.8186554963698668
6	0.7218778075252198	0.695838817404743
7	0.5369570569758549	0.6038531076861177
8	0.41829434869809234	0.5376079661330568
9	0.33763966302561543	0.4881908988879786
>10	4.018301480524065	15.065854158386363
>50	1.1109573286523662	12.69468507157995
>100	1.6744733203051734	49.45793090785733
>500	0.005733562647880901	0.5875896231630677
>1k	0.0016381607565374005	0.5435511055715688
>5k	5.460535855124668E-4	0.6472433920121101
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9263	0.40354217937173237	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5493	0.23930229853059765	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT	3130	0.13635830955775907	Illumina Single End Adapter 2 (95% over 21bp)
TTCAAAGGGACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCC	2657	0.11575208578113924	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1424138383208672	0.0
2	0.0	0.0	0.0	0.3826310009092006	0.0
3	0.0	0.0	0.0	0.5104941442165561	0.0
4	0.0	0.0	0.0	0.6609674992365242	0.0
5	0.0	0.0	0.0	1.0803237573205462	0.0
6	0.0	0.0	0.0	1.3959082922842543	0.0
7	0.0	0.0	0.0	1.6193529471474322	0.0
8	0.0	0.0	0.0	2.104230897747387	0.0
9	0.0	0.0	0.0	2.2794055823262207	0.0
10	0.0	0.0	0.0	2.642345223516537	0.0
11	0.0	0.0	0.0	3.0862721162940336	0.0
12	0.0	0.0	0.0	3.4549623315615468	0.0
13	4.3564955130274464E-5	0.0	0.0	3.5933246290552985	0.0
14	4.3564955130274464E-5	0.0	0.0	3.6489135118015286	0.0
15	4.3564955130274464E-5	0.0	0.0	3.7490693436460294	0.0
16	4.3564955130274464E-5	0.0	0.0	3.943630433257835	0.0
17	8.712991026054893E-5	0.0	0.0	4.190992248487533	0.0
18	8.712991026054893E-5	0.0	0.0	4.489630015905565	0.0
19	8.712991026054893E-5	0.0	0.0	4.654654065939045	0.0
20	8.712991026054893E-5	0.0	0.0	4.8486923760892875	0.0
21	8.712991026054893E-5	0.0	0.0	5.09039074715205	0.0
22	8.712991026054893E-5	0.0	0.0	5.350125009638746	0.0
23	8.712991026054893E-5	0.0	0.0	5.632905133389358	0.0
24	1.306948653908234E-4	0.0	0.0	5.838793111335035	0.0
25	1.306948653908234E-4	0.0	0.0	6.021809487837318	0.0
26	1.7425982052109786E-4	0.0	0.0	6.204128825057516	0.0
27	1.7425982052109786E-4	0.0	0.0	6.391632391938218	0.0
28	1.7425982052109786E-4	0.0	0.0	6.585714267043591	0.0
29	2.613897307816468E-4	0.0	0.0	6.812992637958232	0.0
30	2.613897307816468E-4	0.0	0.0	7.044758199251293	0.0
31	2.613897307816468E-4	0.0	0.0	7.259707687864067	0.0
32	2.613897307816468E-4	0.0	0.0	7.4536153031489185	0.0
33	3.0495468591192125E-4	0.0	0.0	7.6646875107550985	0.0
34	3.0495468591192125E-4	0.0	0.0	7.880900383066651	0.0
35	3.0495468591192125E-4	0.0	0.0	8.146036699989502	0.0
36	3.0495468591192125E-4	0.0	0.0	8.359200025441934	0.0
37	3.0495468591192125E-4	0.0	0.0	8.57127422701611	0.0
38	3.0495468591192125E-4	0.0	0.0	8.805000211290032	0.0
39	3.0495468591192125E-4	0.0	0.0	9.127032359613022	0.0
40	3.0495468591192125E-4	0.0	0.0	9.380449703605828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCG	35	1.020162E-7	46.000004	10
TTTACGC	25	3.418229E-5	46.0	23
CGATTCG	80	0.0	46.0	10
TATGCGT	40	5.6152203E-9	46.0	20
CCCAACG	30	1.8622213E-6	46.0	23
GTGTCGA	25	3.418229E-5	46.0	33
CGAATGT	50	1.6370905E-11	46.0	18
TATCGCC	20	6.3127757E-4	46.0	34
TAGTCCG	20	6.3127757E-4	46.0	15
CGAAGTA	30	1.8622213E-6	46.0	39
TGCGTAG	165	0.0	44.606064	1
TTACCGG	120	0.0	44.083332	2
AATACGG	420	0.0	43.261906	2
ACGCGAG	70	0.0	42.714287	1
TACGGGT	240	0.0	42.166668	4
TAACGCG	60	1.8189894E-12	42.166668	1
CGACGGT	290	0.0	42.034485	28
ACGGGAT	1285	0.0	41.88327	5
TGTGACG	105	0.0	41.619045	1
TACGGGA	1000	0.0	41.4	4
>>END_MODULE