FastQCFastQC Report
Thu 26 May 2016
SRR1544751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544751_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1185839
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.3683467991860615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40540.34186765657058No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT17720.14943006597016967Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT14840.12514346382603372TruSeq Adapter, Index 22 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.1063382128602618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA351.0195981E-746.0000046
CGTTGTA351.0195981E-746.00000446
CGTCTCG351.0195981E-746.00000429
GTTAGCG351.0195981E-746.0000041
ACGATAT351.0195981E-746.00000434
GGACGAT351.0195981E-746.0000048
AGTTCGG351.0195981E-746.0000042
TTACGTT351.0195981E-746.00000414
CATCGAT351.0195981E-746.00000410
TTACCCG351.0195981E-746.00000421
CAATCGT351.0195981E-746.00000442
GCATCGA351.0195981E-746.0000049
GCCCCGC253.417222E-546.028
AGCGTTA301.8614537E-646.011
CGGAATA206.3115364E-446.016
CTATGCG453.092282E-1046.01
CGGCGAA453.092282E-1046.06
AATCCGG253.417222E-546.02
CAAACGA206.3115364E-446.029
CGAACCC206.3115364E-446.029
Produced by FastQC (version 0.11.5)