##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544751_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1185839
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69849195379811	33.0	31.0	34.0	31.0	34.0
2	33.08833323916653	34.0	33.0	34.0	31.0	34.0
3	33.13522662013983	34.0	33.0	34.0	31.0	34.0
4	36.44443722967452	37.0	37.0	37.0	35.0	37.0
5	36.443908490107006	37.0	37.0	37.0	35.0	37.0
6	36.52266201398335	37.0	37.0	37.0	35.0	37.0
7	36.622606441515245	37.0	37.0	37.0	35.0	37.0
8	36.619455929514885	37.0	37.0	37.0	35.0	37.0
9	38.36419530813205	39.0	39.0	39.0	37.0	39.0
10	38.23755585707672	39.0	39.0	39.0	37.0	39.0
11	38.004100050681416	39.0	38.0	39.0	35.0	39.0
12	37.20060227400178	39.0	35.0	39.0	35.0	39.0
13	37.06841822540834	39.0	35.0	39.0	35.0	39.0
14	38.17047761121029	40.0	35.0	41.0	35.0	41.0
15	38.21366306893263	40.0	35.0	41.0	35.0	41.0
16	38.285846560958106	40.0	35.0	41.0	35.0	41.0
17	38.27212378746187	40.0	35.0	41.0	35.0	41.0
18	38.317003404340724	40.0	36.0	41.0	35.0	41.0
19	38.288034885005466	40.0	36.0	41.0	35.0	41.0
20	38.13991444032453	40.0	35.0	41.0	35.0	41.0
21	38.05363375635309	40.0	35.0	41.0	35.0	41.0
22	37.975780860639595	40.0	35.0	41.0	35.0	41.0
23	37.924930787400314	40.0	35.0	41.0	35.0	41.0
24	37.882305270782965	40.0	35.0	41.0	35.0	41.0
25	37.880724955074	40.0	35.0	41.0	35.0	41.0
26	37.82597047322613	40.0	35.0	41.0	35.0	41.0
27	37.79376542684125	40.0	35.0	41.0	35.0	41.0
28	37.806757915703564	40.0	35.0	41.0	35.0	41.0
29	37.78956502526903	40.0	35.0	41.0	35.0	41.0
30	37.75734058333383	40.0	35.0	41.0	35.0	41.0
31	37.610205938580194	40.0	35.0	41.0	35.0	41.0
32	37.49411429376163	40.0	35.0	41.0	34.0	41.0
33	37.36797828372992	40.0	35.0	41.0	34.0	41.0
34	37.24166771374529	39.0	35.0	41.0	34.0	41.0
35	37.11440760507961	39.0	35.0	41.0	34.0	41.0
36	37.0454496774014	39.0	35.0	41.0	34.0	41.0
37	37.000492478321256	39.0	35.0	41.0	34.0	41.0
38	36.90440607873413	39.0	35.0	41.0	33.0	41.0
39	36.91710847762639	39.0	35.0	41.0	33.0	41.0
40	36.81493524837689	39.0	35.0	41.0	33.0	41.0
41	36.81388367223544	39.0	35.0	41.0	33.0	41.0
42	36.81243322238517	39.0	35.0	41.0	33.0	41.0
43	36.76030219953974	39.0	35.0	41.0	33.0	41.0
44	36.674124396313495	39.0	35.0	41.0	33.0	41.0
45	36.59921456454038	38.0	35.0	41.0	33.0	41.0
46	36.54998865781948	38.0	35.0	41.0	33.0	41.0
47	36.488547770818805	38.0	35.0	41.0	33.0	41.0
48	36.44218987569139	38.0	35.0	41.0	33.0	41.0
49	36.45261877877182	38.0	35.0	41.0	33.0	41.0
50	36.31434537066161	38.0	35.0	40.0	33.0	41.0
51	36.24485448699191	37.0	35.0	40.0	33.0	41.0
52	36.03410834017097	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	4.0
13	8.0
14	8.0
15	14.0
16	30.0
17	66.0
18	151.0
19	313.0
20	534.0
21	883.0
22	1515.0
23	2497.0
24	4052.0
25	6612.0
26	8687.0
27	9658.0
28	9759.0
29	9748.0
30	11187.0
31	12999.0
32	16493.0
33	23450.0
34	61961.0
35	280055.0
36	43858.0
37	54714.0
38	109020.0
39	515673.0
40	1886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.075789377816044	23.039974229216615	29.596091880938307	13.288144512029035
2	30.36078253455992	26.181041439858195	30.389538546126417	13.068637479455475
3	29.384343068494122	25.061580872276927	31.46261844989075	14.091457609338198
4	25.675492204253697	28.559273223430836	30.538125327299916	15.227109245015555
5	21.981061510036355	34.49970864510275	28.68627191380955	14.83295793105135
6	21.133054318503607	43.56375528212514	25.203084061158386	10.10010633821286
7	87.76123908894884	4.785388235671116	5.63550363919554	1.8178690361845071
8	88.32033690914196	3.614318638533561	5.9017286495046966	2.1636158028197756
9	84.88707151645374	4.747356091341236	7.5371951841691835	2.8283772080358296
10	50.723411862824555	26.48403366730222	12.69944739547274	10.09310707440049
11	43.53904703758268	20.97797424439574	23.11814672986805	12.364831988153535
12	41.91462753375458	20.55270572143436	24.534021903479307	12.99864484133175
13	19.55982220183347	42.150494291383566	24.95423071766066	13.335452789122302
14	14.895613991444032	45.08571568315766	26.731790740564275	13.286879584834029
15	12.87248943574971	24.92800456048418	49.640971497817155	12.558534505948954
16	14.361140087313709	20.612663270477697	48.27172997346183	16.75446666874677
17	14.509979853926207	19.6426327688666	30.04075595422313	35.80663142298406
18	18.42518250791212	23.131807943574128	39.43553888850004	19.007470660013713
19	27.753177286292658	23.643513158194324	29.170570372537924	19.43273918297509
20	30.484408085751944	22.835140352105135	28.69858387184095	17.98186769030197
21	20.16842083959121	30.3572407384139	31.494157301286265	17.98018112070863
22	20.28749265288121	23.46456812434066	27.26685494405227	28.981084278725866
23	16.590110461875515	31.319513019895616	27.67129433253587	24.419082185693
24	16.17023896161283	23.07370562108347	43.64858973267028	17.10746568463341
25	14.390486398237872	26.33325434565738	40.08722937936769	19.189029876737063
26	14.999000707515945	36.04991908682376	28.557670982317163	20.393409223343134
27	15.052886606023247	38.46070166354792	28.89043116308369	17.595980567345144
28	13.111307690167045	30.4101146951652	40.109239112560815	16.36933850210695
29	13.99093806157497	25.066724909536624	38.59731380060868	22.345023228279725
30	17.26726815360264	34.28922475985357	31.296238359507488	17.147268727036302
31	28.288241489780653	28.696391331369604	26.91090443137728	16.104462747472464
32	29.91080576705607	25.438782161828037	28.394748359600246	16.255663711515645
33	29.30836310831403	28.43219020457246	25.128284699693637	17.131161987419876
34	19.12780740049872	27.97909328332092	27.02963893074861	25.863460385431747
35	17.9928303926587	26.427280600486235	33.72026050753939	21.859628499315676
36	30.859163849392708	25.055087579342562	27.22410040486103	16.861648166403704
37	18.026646113005224	35.61630204437533	31.419020625902842	14.938031216716603
38	17.476655768616144	35.142797631044346	27.035373267365976	20.34517333297353
39	19.400947346140583	35.61604905893633	27.952529812225773	17.030473782697314
40	25.456575471037805	25.43380678152768	27.923605143699948	21.18601260373457
41	15.280067530246516	25.020344245719695	30.42048709816425	29.279101125869534
42	19.84358753591339	24.08539439165013	27.962733558265494	28.10828451417098
43	19.39327345449087	25.365838026915966	29.282980235934218	25.95790828265894
44	16.43022366442662	30.129300857873627	32.75107329072496	20.689402186974792
45	16.388312410032054	40.12736973568924	24.25464165034208	19.22967620393662
46	20.157964108112484	34.747381389885135	27.35497820530443	17.73967629669795
47	18.455625089071955	29.160788268896535	28.326695276508868	24.056891365522638
48	20.060986356495274	25.65567501153192	34.80632699717246	19.47701163480034
49	20.905704737321003	25.47791057639359	35.034857177070414	18.581527509214997
50	18.928117560646935	35.44722344264272	28.016703785252467	17.607955211457877
51	17.49925580116694	34.186765657058	26.364371554654554	21.94960698712051
52	18.787541985041813	26.477034403489853	36.46161072455873	18.273812886909603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4581.0
1	4199.0
2	3817.0
3	5940.0
4	8063.0
5	5659.5
6	3256.0
7	3503.5
8	3751.0
9	3815.5
10	3880.0
11	3823.0
12	3766.0
13	3767.0
14	3708.0
15	3648.0
16	3664.5
17	3681.0
18	3818.5
19	3956.0
20	4450.5
21	4945.0
22	6102.5
23	7260.0
24	7908.0
25	8556.0
26	9921.0
27	11286.0
28	15438.5
29	19591.0
30	22073.5
31	24556.0
32	25698.0
33	26840.0
34	32580.5
35	38321.0
36	43267.0
37	48213.0
38	56026.0
39	72517.5
40	81196.0
41	104012.5
42	126829.0
43	135760.5
44	144692.0
45	136138.0
46	127584.0
47	114113.0
48	100642.0
49	91374.0
50	82106.0
51	72544.0
52	62982.0
53	55292.0
54	47602.0
55	40548.0
56	33494.0
57	28502.5
58	23511.0
59	20623.5
60	17736.0
61	15484.0
62	13232.0
63	11222.5
64	7716.0
65	6219.0
66	4687.5
67	3156.0
68	2618.5
69	2081.0
70	1661.5
71	1242.0
72	1196.5
73	1151.0
74	873.5
75	596.0
76	525.0
77	454.0
78	373.0
79	292.0
80	227.5
81	163.0
82	117.0
83	71.0
84	43.0
85	15.0
86	8.0
87	1.0
88	2.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1185839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.186738763616763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46801942884504	12.215811155022688
2	10.389267773482397	3.3633672678924387
3	3.606556259929193	1.751351520472817
4	1.7648352840378436	1.1426771081413585
5	1.003172373876751	0.811904457541013
6	0.6666741588350575	0.6474768269710214
7	0.44511119539451127	0.5043429048428492
8	0.33463503577841386	0.4333319924238988
9	0.2596239249985079	0.37822181856621107
>10	3.3493487188549107	13.09068065148534
>50	1.084053930330563	12.39825197836534
>100	1.6192662431291034	51.48392440735097
>500	0.006814652366681238	0.6807638784153961
>1k	0.0026210201410312457	1.097894032508639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4368	0.3683467991860615	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4054	0.34186765657058	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	1772	0.14943006597016967	Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1484	0.12514346382603372	TruSeq Adapter, Index 22 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1261	0.1063382128602618	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15626067282320788	0.0
2	0.0	0.0	0.0	0.4584939439502327	0.0
3	8.432847966713862E-5	0.0	0.0	0.6001657897910256	0.0
4	8.432847966713862E-5	0.0	0.0	0.7716055889543184	0.0
5	8.432847966713862E-5	0.0	0.0	1.2631562969340695	0.0
6	8.432847966713862E-5	0.0	0.0	1.6210463646414057	0.0
7	8.432847966713862E-5	0.0	0.0	1.879766140260187	0.0
8	8.432847966713862E-5	0.0	0.0	2.4439236692333446	0.0
9	8.432847966713862E-5	0.0	0.0	2.650444115938167	0.0
10	8.432847966713862E-5	0.0	0.0	3.0575820157711124	0.0
11	8.432847966713862E-5	0.0	0.0	3.585899940885736	0.0
12	8.432847966713862E-5	0.0	0.0	4.004422185473745	0.0
13	8.432847966713862E-5	0.0	0.0	4.1599239019799485	0.0
14	8.432847966713862E-5	0.0	0.0	4.211111289137902	0.0
15	8.432847966713862E-5	0.0	0.0	4.312221136258801	0.0
16	8.432847966713862E-5	0.0	0.0	4.5462326673351106	0.0
17	8.432847966713862E-5	0.0	0.0	4.8129636485222695	0.0
18	8.432847966713862E-5	0.0	0.0	5.195899274690746	0.0
19	1.6865695933427724E-4	0.0	0.0	5.365315190342028	0.0
20	1.6865695933427724E-4	0.0	0.0	5.5376826027816595	0.0
21	6.74627837337109E-4	0.0	0.0	5.7793680255076785	0.0
22	6.74627837337109E-4	0.0	0.0	6.018017622965681	0.0
23	6.74627837337109E-4	0.0	0.0	6.329358369896757	0.0
24	7.589563170042476E-4	0.0	0.0	6.542793751934284	0.0
25	7.589563170042476E-4	0.0	0.0	6.710270112553222	0.0
26	7.589563170042476E-4	0.0	0.0	6.886516635057542	0.0
27	7.589563170042476E-4	0.0	0.0	7.055173594391818	0.0
28	7.589563170042476E-4	0.0	0.0	7.245755958439552	0.0
29	7.589563170042476E-4	0.0	0.0	7.427399503642569	0.0
30	7.589563170042476E-4	0.0	0.0	7.673638664270613	0.0
31	7.589563170042476E-4	0.0	0.0	7.903096457444898	0.0
32	7.589563170042476E-4	0.0	0.0	8.09899151571166	0.0
33	7.589563170042476E-4	0.0	0.0	8.2967418005311	0.0
34	7.589563170042476E-4	0.0	0.0	8.492552530318196	0.0
35	7.589563170042476E-4	0.0	0.0	8.770752184740086	0.0
36	8.432847966713862E-4	0.0	0.0	8.991186830589987	0.0
37	8.432847966713862E-4	0.0	0.0	9.21330804603323	0.0
38	8.432847966713862E-4	0.0	0.0	9.426912085030093	0.0
39	9.276132763385249E-4	0.0	0.0	9.637311641799604	0.0
40	9.276132763385249E-4	0.0	0.0	9.863733609705871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	35	1.0195981E-7	46.000004	6
CGTTGTA	35	1.0195981E-7	46.000004	46
CGTCTCG	35	1.0195981E-7	46.000004	29
GTTAGCG	35	1.0195981E-7	46.000004	1
ACGATAT	35	1.0195981E-7	46.000004	34
GGACGAT	35	1.0195981E-7	46.000004	8
AGTTCGG	35	1.0195981E-7	46.000004	2
TTACGTT	35	1.0195981E-7	46.000004	14
CATCGAT	35	1.0195981E-7	46.000004	10
TTACCCG	35	1.0195981E-7	46.000004	21
CAATCGT	35	1.0195981E-7	46.000004	42
GCATCGA	35	1.0195981E-7	46.000004	9
GCCCCGC	25	3.417222E-5	46.0	28
AGCGTTA	30	1.8614537E-6	46.0	11
CGGAATA	20	6.3115364E-4	46.0	16
CTATGCG	45	3.092282E-10	46.0	1
CGGCGAA	45	3.092282E-10	46.0	6
AATCCGG	25	3.417222E-5	46.0	2
CAAACGA	20	6.3115364E-4	46.0	29
CGAACCC	20	6.3115364E-4	46.0	29
>>END_MODULE