FastQCFastQC Report
Thu 26 May 2016
SRR1544750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544750_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1564119
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82840.5296272214582138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31300.20011265127525463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC351.0198892E-746.00000446
TATATCG351.0198892E-746.00000431
ATCGTTA351.0198892E-746.00000445
GTCCGAT351.0198892E-746.00000441
ACGTCCA700.046.00000418
CAACGTA301.8618375E-646.09
ATTACCG405.6134013E-946.022
TTATCCG405.6134013E-946.01
ATCGTAA206.3121575E-446.014
TACGCTT206.3121575E-446.035
CTAACCG206.3121575E-446.01
CGTAGAC253.417725E-546.011
CACTACG206.3121575E-446.01
TCTAACG206.3121575E-446.01
ACGCTTA206.3121575E-446.018
CGTACCG301.8618375E-646.030
CTCGACA206.3121575E-446.033
CCAACGT206.3121575E-446.045
GCTCGAC206.3121575E-446.032
CAATCGA206.3121575E-446.034
Produced by FastQC (version 0.11.5)