##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544750_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1564119
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.706481412219915	33.0	31.0	34.0	31.0	34.0
2	33.12568161373911	34.0	33.0	34.0	31.0	34.0
3	33.17053689648933	34.0	33.0	34.0	31.0	34.0
4	36.48531729363303	37.0	37.0	37.0	35.0	37.0
5	36.48068401445158	37.0	37.0	37.0	35.0	37.0
6	36.55235503181024	37.0	37.0	37.0	35.0	37.0
7	36.631033188651244	37.0	37.0	37.0	35.0	37.0
8	36.63147944625697	37.0	37.0	37.0	35.0	37.0
9	38.362340077705085	39.0	39.0	39.0	37.0	39.0
10	38.26752440191571	39.0	39.0	39.0	37.0	39.0
11	38.107052596381735	39.0	39.0	39.0	35.0	39.0
12	37.4377672031348	39.0	35.0	39.0	35.0	39.0
13	37.321444212364916	39.0	35.0	39.0	35.0	39.0
14	38.421033821595415	40.0	35.0	41.0	35.0	41.0
15	38.46997319257678	40.0	35.0	41.0	35.0	41.0
16	38.56990612606841	40.0	35.0	41.0	35.0	41.0
17	38.5219276794157	40.0	35.0	41.0	35.0	41.0
18	38.524213950473076	40.0	36.0	41.0	35.0	41.0
19	38.49963781528132	40.0	36.0	41.0	35.0	41.0
20	38.38173118541492	40.0	35.0	41.0	35.0	41.0
21	38.323843006830046	40.0	35.0	41.0	35.0	41.0
22	38.27530386115123	40.0	35.0	41.0	35.0	41.0
23	38.21198451012998	40.0	35.0	41.0	35.0	41.0
24	38.148388965289726	40.0	35.0	41.0	35.0	41.0
25	38.13715324729129	40.0	35.0	41.0	35.0	41.0
26	38.097387730728926	40.0	35.0	41.0	35.0	41.0
27	38.05345501205471	40.0	35.0	41.0	35.0	41.0
28	38.03930647220576	40.0	35.0	41.0	35.0	41.0
29	37.986171128923054	40.0	35.0	41.0	35.0	41.0
30	37.91786878108379	40.0	35.0	41.0	35.0	41.0
31	37.81153096407626	40.0	35.0	41.0	34.0	41.0
32	37.69807476285372	40.0	35.0	41.0	34.0	41.0
33	37.55493475880032	40.0	35.0	41.0	34.0	41.0
34	37.37628594755258	40.0	35.0	41.0	34.0	41.0
35	37.261381646792856	40.0	35.0	41.0	34.0	41.0
36	37.18800743421696	40.0	35.0	41.0	34.0	41.0
37	37.169526103832254	40.0	35.0	41.0	34.0	41.0
38	37.047255995227985	40.0	35.0	41.0	33.0	41.0
39	37.0466403131731	40.0	35.0	41.0	33.0	41.0
40	36.96140255313055	40.0	35.0	41.0	33.0	41.0
41	36.91562023094151	39.0	35.0	41.0	33.0	41.0
42	36.91239733038215	39.0	35.0	41.0	33.0	41.0
43	36.87559961869909	39.0	35.0	41.0	33.0	41.0
44	36.77572358624887	39.0	35.0	41.0	33.0	41.0
45	36.6896284745598	39.0	35.0	41.0	33.0	41.0
46	36.63057606230728	39.0	35.0	41.0	33.0	41.0
47	36.60399176788978	39.0	35.0	41.0	33.0	41.0
48	36.533546360603	38.0	35.0	41.0	33.0	41.0
49	36.49201946910689	38.0	35.0	41.0	33.0	41.0
50	36.37931896486137	38.0	35.0	41.0	33.0	41.0
51	36.317723267858774	38.0	35.0	40.0	33.0	41.0
52	36.12002731249988	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	5.0
13	8.0
14	8.0
15	26.0
16	42.0
17	112.0
18	221.0
19	405.0
20	741.0
21	1328.0
22	2007.0
23	3330.0
24	5196.0
25	8444.0
26	11801.0
27	13529.0
28	13440.0
29	13639.0
30	14154.0
31	16774.0
32	21380.0
33	30651.0
34	86077.0
35	287045.0
36	64060.0
37	85623.0
38	168857.0
39	713011.0
40	2201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56683155181927	24.16747063362826	28.864619635718253	14.40107817883422
2	31.084591389785558	25.013889608143625	30.63155680609979	13.269962195971022
3	28.672818372515135	24.92815444349183	32.90152475610871	13.497502427884323
4	25.780007787131286	27.211932084451373	31.935997197144207	15.07206293127313
5	22.936170457618633	32.247674249849275	30.090357575094988	14.7257977174371
6	22.167750663472535	42.111821415122506	25.994057996865966	9.72636992453899
7	88.23235316494461	3.5795869751598186	6.5236724315733	1.6643874283222695
8	88.94719647290263	2.534206156948416	6.522010153958874	1.9965872161900724
9	84.53941164323176	4.737683002380254	7.8935809871243805	2.829324367263616
10	44.8782989018099	29.884490885923643	13.903609635839729	11.33360057642673
11	41.037734341185036	20.591208213697296	23.534910067584374	14.836147377533296
12	37.8936641010051	20.237782419368347	26.361868885935145	15.5066845936914
13	23.60057003335424	33.59392731627197	26.837408151170084	15.968094499203705
14	16.92754835150011	35.4336210991619	31.27594511670787	16.362885432630126
15	16.493757827889056	24.1514232612736	44.587080650513165	14.767738260324181
16	21.114505993469805	20.599263866751826	40.32116482185818	17.965065317920185
17	19.560596092752533	21.125438665472384	30.314253583007435	28.99971165876765
18	22.233474562996804	22.537671366436953	37.018347069500464	18.210507001065775
19	27.52821236747332	24.49947862023286	28.766992792747864	19.205316219545956
20	28.23704590251765	24.980068652065476	29.334660598074695	17.44822484734218
21	22.34753238084826	28.156745106989938	31.50086406469073	17.994858447471067
22	21.530714734620577	23.7887270725565	28.967680847812733	25.71287734501019
23	18.691800304196803	27.973255231858957	29.81301294850328	23.52193151544096
24	18.400070582864856	23.821652956073038	39.93653935538153	17.84173710568058
25	19.152251203393092	25.09463793995214	34.94663769188917	20.806473164765595
26	18.040826816885417	31.116302531968476	29.513035772853602	21.32983487829251
27	16.416334051309395	32.712920180625645	31.42772384965594	19.44302191840902
28	16.39255069467221	28.54578200252027	36.08101429622682	18.9806530065807
29	18.384087144264598	24.721776284285273	35.163500986817496	21.730635584632626
30	19.941257666456323	30.414629577417063	32.29076560031558	17.353347155811036
31	27.20982227055614	26.108051880963025	29.490083555023627	17.19204229345721
32	28.961798942407835	24.61315283555791	29.688981464965263	16.736066757068997
33	27.669889567226026	26.200627957335726	27.570152910360402	18.55932956507785
34	19.897654845954815	26.08938322467792	31.103068244807456	22.90989368455981
35	19.18530495441843	27.36556489627707	33.4297454349701	20.019384714334397
36	27.807219271679458	27.751021501560942	26.925636732243518	17.516122494516082
37	19.381773381692824	34.06467154992683	29.165491883929544	17.3880631844508
38	20.03070098886338	32.39305960735724	26.986885268959714	20.589354134819665
39	20.012415935104684	31.92506452514163	28.756315855762892	19.3062036839908
40	24.39692887817359	25.212531783067654	27.878505407836617	22.512033930922133
41	17.232320558729867	25.958382961910186	30.04138431922379	26.767912160136152
42	19.350509775790716	25.664159824156602	28.32987771390796	26.655452686144727
43	19.797342785299584	24.459967560013016	30.381000422602117	25.361689232085283
44	18.80119095797698	28.11378162403244	31.431623808674402	21.65340360931617
45	17.597957700149415	34.81992099066631	26.781977586104382	20.800143723079895
46	20.072449730487257	31.431687742428803	28.447579755760273	20.048282771323667
47	19.097779644643406	28.55805728336527	29.591034953222866	22.753128118768455
48	20.114837809655146	26.83977370008292	32.805943793279155	20.239444696982776
49	21.734215874879084	26.036829678560263	33.144600890341465	19.084353556219188
50	19.208768642283612	33.00324335936076	29.077135435347312	18.710852563008313
51	18.34304167393913	31.675531081714368	28.24708350195861	21.73434374238789
52	19.24546661730981	26.545678429838137	34.60286589447478	19.605989058377272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3646.0
1	4194.0
2	4742.0
3	10189.0
4	15636.0
5	10384.0
6	5132.0
7	5216.0
8	5300.0
9	5354.0
10	5408.0
11	5262.0
12	5116.0
13	5274.0
14	5223.5
15	5015.0
16	4896.5
17	4778.0
18	4898.0
19	5018.0
20	5762.0
21	6506.0
22	8330.5
23	10155.0
24	11014.5
25	11874.0
26	13787.0
27	15700.0
28	19788.0
29	23876.0
30	28273.5
31	32671.0
32	35693.5
33	38716.0
34	45011.5
35	51307.0
36	59563.0
37	67819.0
38	72132.5
39	90287.5
40	104129.0
41	116199.5
42	128270.0
43	139502.0
44	150734.0
45	143175.0
46	135616.0
47	129977.5
48	124339.0
49	112760.5
50	101182.0
51	93687.5
52	86193.0
53	77995.5
54	69798.0
55	64476.0
56	59154.0
57	53509.5
58	47865.0
59	44991.0
60	42117.0
61	37832.5
62	33548.0
63	29945.5
64	22647.5
65	18952.0
66	15720.0
67	12488.0
68	10370.0
69	8252.0
70	7012.5
71	5773.0
72	4617.5
73	3462.0
74	2999.5
75	2537.0
76	1977.5
77	1418.0
78	1203.5
79	989.0
80	646.0
81	303.0
82	253.0
83	203.0
84	127.0
85	51.0
86	70.0
87	89.0
88	53.0
89	10.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1564119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.470424464558896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73968581129697	12.474647741243897
2	8.654804482470766	2.8509660696812094
3	3.0056761077698733	1.4851428389385923
4	1.5408701766848256	1.0151514341911576
5	1.0091233956903474	0.831034533206852
6	0.7286539184003362	0.720074359429056
7	0.597866799734634	0.6892983975427794
8	0.48643189556911604	0.6409391834498661
9	0.437410468551465	0.6483902474055795
>10	5.265926249015567	20.098349595471102
>50	1.0831251965569717	12.588756433504274
>100	1.444166920057301	44.49954875921445
>500	0.004302772513713519	0.4668944951608089
>1k	0.0015646445504412796	0.4571017584056767
>5k	3.911611376103199E-4	0.5337041531547041
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8284	0.5296272214582138	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3130	0.20011265127525463	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08867611735424223	0.0
2	0.0	0.0	0.0	0.25950710911382063	0.0
3	0.0	0.0	0.0	0.3454980087832192	0.0
4	0.0	0.0	0.0	0.4461297382104559	0.0
5	0.0	0.0	0.0	0.7274382575750311	0.0
6	6.393375440103982E-5	0.0	0.0	0.9580473096995816	0.0
7	6.393375440103982E-5	0.0	0.0	1.133481531776035	0.0
8	6.393375440103982E-5	0.0	0.0	1.5472607902595648	0.0
9	6.393375440103982E-5	0.0	0.0	1.6975051131020082	0.0
10	6.393375440103982E-5	0.0	0.0	1.998824897594109	0.0
11	6.393375440103982E-5	0.0	0.0	2.382874960281155	0.0
12	1.2786750880207964E-4	0.0	0.0	2.68566522112448	0.0
13	1.2786750880207964E-4	0.0	0.0	2.8014492503447626	0.0
14	1.2786750880207964E-4	0.0	0.0	2.848696294847131	0.0
15	1.2786750880207964E-4	0.0	0.0	2.918831623425072	0.0
16	1.2786750880207964E-4	0.0	0.0	3.066838264863479	0.0
17	1.2786750880207964E-4	0.0	0.0	3.2413134806239166	0.0
18	1.2786750880207964E-4	0.0	0.0	3.479850318294196	0.0
19	1.2786750880207964E-4	0.0	0.0	3.615837413905208	0.0
20	1.2786750880207964E-4	0.0	0.0	3.778484885101453	0.0
21	1.2786750880207964E-4	0.0	0.0	3.964659977917281	0.0
22	1.2786750880207964E-4	0.0	0.0	4.1718053421766506	0.0
23	1.2786750880207964E-4	0.0	0.0	4.386558823209743	0.0
24	1.9180126320311944E-4	0.0	0.0	4.5579012850045295	0.0
25	1.9180126320311944E-4	0.0	0.0	4.702199768687676	0.0
26	1.9180126320311944E-4	0.0	0.0	4.840424545702724	0.0
27	2.557350176041593E-4	0.0	0.0	5.0049260957766	0.0
28	3.196687720051991E-4	0.0	0.0	5.158047437567091	0.0
29	3.196687720051991E-4	0.0	0.0	5.347099549330966	0.0
30	3.196687720051991E-4	0.0	0.0	5.5708037559802035	0.0
31	3.196687720051991E-4	0.0	0.0	5.754677233637594	0.0
32	3.196687720051991E-4	0.0	0.0	5.922695140203527	0.0
33	3.196687720051991E-4	0.0	0.0	6.1021571888072454	0.0
34	3.196687720051991E-4	0.0	0.0	6.302078038819297	0.0
35	3.196687720051991E-4	0.0	0.0	6.53901653262955	0.0
36	3.196687720051991E-4	0.0	0.0	6.722058871479727	0.0
37	3.196687720051991E-4	0.0	0.0	6.910791314471597	0.0
38	3.196687720051991E-4	0.0	0.0	7.161091962951668	0.0
39	3.196687720051991E-4	0.0	0.0	7.625890357447227	0.0
40	3.196687720051991E-4	0.0	0.0	7.889425293088314	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	35	1.0198892E-7	46.000004	46
TATATCG	35	1.0198892E-7	46.000004	31
ATCGTTA	35	1.0198892E-7	46.000004	45
GTCCGAT	35	1.0198892E-7	46.000004	41
ACGTCCA	70	0.0	46.000004	18
CAACGTA	30	1.8618375E-6	46.0	9
ATTACCG	40	5.6134013E-9	46.0	22
TTATCCG	40	5.6134013E-9	46.0	1
ATCGTAA	20	6.3121575E-4	46.0	14
TACGCTT	20	6.3121575E-4	46.0	35
CTAACCG	20	6.3121575E-4	46.0	1
CGTAGAC	25	3.417725E-5	46.0	11
CACTACG	20	6.3121575E-4	46.0	1
TCTAACG	20	6.3121575E-4	46.0	1
ACGCTTA	20	6.3121575E-4	46.0	18
CGTACCG	30	1.8618375E-6	46.0	30
CTCGACA	20	6.3121575E-4	46.0	33
CCAACGT	20	6.3121575E-4	46.0	45
GCTCGAC	20	6.3121575E-4	46.0	32
CAATCGA	20	6.3121575E-4	46.0	34
>>END_MODULE