FastQCFastQC Report
Thu 26 May 2016
SRR1544749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544749_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1792499
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83770.46733638345125994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40450.22566260845891684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT206.312404E-446.00000427
GAATCGT405.6152203E-946.00000432
ACGCTAA206.312404E-446.00000434
TCGTACA800.046.00000434
ACGTATA206.312404E-446.00000416
TACGTCG206.312404E-446.00000423
GTACGAT206.312404E-446.0000049
GTCGACA301.8619903E-646.044
ACGTTAC253.4179262E-546.043
TAGCGCG900.046.01
CGACACG453.110472E-1046.017
CTCGGTA301.8619903E-646.016
CGCTATA253.4179262E-546.030
TACGAAC253.4179262E-546.040
CGTACCG253.4179262E-546.014
TTACGCC301.8619903E-646.017
ATACCGG301.8619903E-646.02
ACGGTCC253.4179262E-546.040
CGGTCTA3500.044.02857231
ACGACGG3600.043.44444727
Produced by FastQC (version 0.11.5)