FastQCFastQC Report
Thu 26 May 2016
SRR1544748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544748_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1543793
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.41372126962617395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32400.20987269666334799No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT21610.13997990663256019TruSeq Adapter, Index 23 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG351.019871E-746.0000041
TTATCCG351.019871E-746.0000041
CCGTATT351.019871E-746.00000441
GTATACG1100.046.0000041
ACGCCGT206.312132E-446.018
CGGTTCA253.4177043E-546.022
CACGCTA301.8618211E-646.026
ATAGCGC206.312132E-446.012
GCCGGAT206.312132E-446.019
ATGTACG800.046.01
CGTTAGT206.312132E-446.012
AATGCGC253.4177043E-546.034
CCCGTAC301.8618211E-646.033
CGTAGTC206.312132E-446.020
GGGCGTA453.110472E-1046.07
ACGGATC253.4177043E-546.013
TATTACG253.4177043E-546.01
AGCGATA301.8618211E-646.041
TGCTCGA301.8618211E-646.032
CGTAAAT206.312132E-446.014
Produced by FastQC (version 0.11.5)