##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544748_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1543793 Sequences flagged as poor quality 0 Sequence length 52 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.661586754182714 33.0 31.0 34.0 31.0 34.0 2 33.09142546960635 34.0 33.0 34.0 31.0 34.0 3 33.14049811082185 34.0 33.0 34.0 31.0 34.0 4 36.467442202419626 37.0 37.0 37.0 35.0 37.0 5 36.46686116597238 37.0 37.0 37.0 35.0 37.0 6 36.51715806458508 37.0 37.0 37.0 35.0 37.0 7 36.606536627643735 37.0 37.0 37.0 35.0 37.0 8 36.60342805026322 37.0 37.0 37.0 35.0 37.0 9 38.3008311347441 39.0 39.0 39.0 37.0 39.0 10 38.19683338375028 39.0 39.0 39.0 37.0 39.0 11 38.05148423396142 39.0 39.0 39.0 35.0 39.0 12 37.4478579705958 39.0 35.0 39.0 35.0 39.0 13 37.33359006032545 39.0 35.0 39.0 35.0 39.0 14 38.53969152600122 40.0 35.0 41.0 35.0 41.0 15 38.545218821435256 40.0 35.0 41.0 35.0 41.0 16 38.606498410084775 40.0 35.0 41.0 35.0 41.0 17 38.59025141323999 40.0 35.0 41.0 35.0 41.0 18 38.6284054921871 40.0 36.0 41.0 35.0 41.0 19 38.59098661543354 40.0 36.0 41.0 35.0 41.0 20 38.47186054088858 40.0 35.0 41.0 35.0 41.0 21 38.38393554058089 40.0 35.0 41.0 35.0 41.0 22 38.30374927208505 40.0 35.0 41.0 35.0 41.0 23 38.23080102060315 40.0 35.0 41.0 35.0 41.0 24 38.19166364920686 40.0 35.0 41.0 35.0 41.0 25 38.19799739991048 40.0 35.0 41.0 35.0 41.0 26 38.14452390961742 40.0 35.0 41.0 35.0 41.0 27 38.123314459904925 40.0 35.0 41.0 35.0 41.0 28 38.06993100758975 40.0 35.0 41.0 35.0 41.0 29 38.05265796645016 40.0 35.0 41.0 35.0 41.0 30 38.022811348412645 40.0 36.0 41.0 35.0 41.0 31 37.904726216532914 40.0 35.0 41.0 35.0 41.0 32 37.806143699317204 40.0 35.0 41.0 35.0 41.0 33 37.68880672473576 40.0 35.0 41.0 35.0 41.0 34 37.54805339835069 40.0 35.0 41.0 34.0 41.0 35 37.40662316774334 40.0 35.0 41.0 34.0 41.0 36 37.33446647316059 40.0 35.0 41.0 34.0 41.0 37 37.295320033190976 40.0 35.0 41.0 34.0 41.0 38 37.19532800058039 40.0 35.0 41.0 33.0 41.0 39 37.20786271216413 40.0 35.0 41.0 34.0 41.0 40 37.12250217483821 40.0 35.0 41.0 33.0 41.0 41 37.071547156905105 40.0 35.0 41.0 33.0 41.0 42 37.0474487188373 40.0 35.0 41.0 33.0 41.0 43 37.00698474471642 40.0 35.0 41.0 33.0 41.0 44 36.912865908836224 39.0 35.0 41.0 33.0 41.0 45 36.83570012300872 39.0 35.0 41.0 33.0 41.0 46 36.7948870088153 39.0 35.0 41.0 33.0 41.0 47 36.73406214434189 39.0 35.0 41.0 33.0 41.0 48 36.68287134350266 39.0 35.0 41.0 33.0 41.0 49 36.64029115302375 39.0 35.0 41.0 33.0 41.0 50 36.551485205594275 39.0 35.0 41.0 33.0 41.0 51 36.47945288001694 38.0 35.0 41.0 33.0 41.0 52 36.25909756035945 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 3.0 11 3.0 12 2.0 13 5.0 14 6.0 15 16.0 16 42.0 17 94.0 18 172.0 19 364.0 20 660.0 21 1154.0 22 1934.0 23 2966.0 24 4954.0 25 7958.0 26 11045.0 27 12784.0 28 12573.0 29 13013.0 30 14086.0 31 16763.0 32 21119.0 33 29057.0 34 73499.0 35 289114.0 36 62318.0 37 79992.0 38 158275.0 39 726934.0 40 2887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.29267136202846 23.78369379832659 29.72684809427171 14.196786745373247 2 30.83282538526862 26.01294344513805 29.8105380708424 13.343693098750933 3 28.215570351724615 26.03263520433115 31.312293811411244 14.43950063253299 4 25.951860126325226 27.634080475815086 31.21487142382431 15.19918797403538 5 23.357989056823033 32.636953270289474 29.164790875460632 14.840266797426857 6 22.55276452218659 41.32088952340113 26.176436866859742 9.949909087552541 7 87.44559665706477 4.139933268255524 6.542975645050858 1.871494429628843 8 88.16900970531671 3.340408979701294 6.1577556058357565 2.332825709146239 9 83.11444604296042 5.5806056900115495 7.865691838219243 3.4392564288087843 10 44.03453053615349 30.956870513080446 14.13667505941535 10.871923891350718 11 39.15797001281908 21.97004391132749 24.12350619545496 14.748479880398474 12 37.65414145549306 20.27629351862588 26.92621355324192 15.143351472639143 13 22.011500246470867 36.20673237927623 26.62928255277748 15.152484821475417 14 15.263380517983952 38.67124672802636 28.92181788620625 17.14355486778344 15 14.131428241998764 26.08523292954431 44.29609410069874 15.48724472775819 16 17.134356743423503 22.23128359825443 42.84874980000557 17.785609858316498 17 16.52339400424798 22.033070495850158 30.718690912577014 30.724844587324856 18 19.397678315680924 23.555683955038013 37.10465068827233 19.941987041008737 19 25.274049046731008 25.264073616087128 28.80574014780479 20.656137189377073 20 28.015284432563174 23.38759147113635 30.14089324151619 18.456230854784287 21 21.20815420202061 27.117236572519758 32.039463840035545 19.63514538542408 22 22.002107795539946 24.2549357329642 28.00219977678354 25.740756694712307 23 17.27375367034311 29.4992269041251 28.197757082717693 25.029262342814096 24 17.365605362895156 23.942005178155362 41.04883232402272 17.64355713492677 25 16.506617143619643 26.839543902582797 36.839654020973015 19.814184932824546 26 16.557984133883235 32.44372788320714 29.620098031277507 21.378189951632116 27 15.751010660107928 35.27921165596683 29.41223337584767 19.557544308077574 28 15.409643650411681 29.830294605559164 36.663918025279294 18.096143718749857 29 15.55124294513578 25.53548306022893 35.08773520802336 23.825538786611936 30 17.40434112604475 31.402267013777106 32.26248596800219 18.930905892175957 31 24.120008317177238 29.269403346174 27.837734722206932 18.772853614441832 32 25.8744533755497 24.820102176911025 30.926166914864883 18.379277532674394 33 25.219508055808 27.52979188272003 26.828532063560335 20.422167997911636 34 19.701864174795457 26.84109851515067 29.118670702613624 24.338366607440246 35 19.874555720877087 25.03425005813603 32.5072726719191 22.583921549067785 36 27.44921113128509 26.08082819393533 27.59042177286722 18.87953890191237 37 18.975406676931428 32.68410985151507 30.913600463274545 17.42688300827896 38 18.807767621695394 31.52832018282244 27.69322052891806 21.970691666564104 39 19.898716991202836 30.846622571808524 29.56160573341115 19.693054703577488 40 23.382927633432722 25.59112523505418 29.138880665996027 21.887066465517073 41 17.395725981397764 25.299311500959003 30.70087764357009 26.60408487407314 42 19.197003743377515 24.901654561200885 29.200546964521795 26.7007947308998 43 19.715985238953667 25.00782164448213 30.38133998534778 24.894853131216426 44 18.03693889012322 27.94979637814137 30.26623387980124 23.747030851934163 45 16.333407393348722 35.687815659223745 27.342979272480182 20.635797674947355 46 20.55294978018426 32.05358490419376 27.832617455837667 19.56084785978431 47 18.287555391169672 29.096258371426735 30.127938136783882 22.488248100619707 48 20.042777755826073 25.634071407241777 33.75426627792716 20.568884559004996 49 21.175831215713504 25.40657976814249 32.915682348605024 20.50190666753898 50 20.34288275694993 31.231648284452646 29.38425034962589 19.041218608971537 51 18.123219887640378 30.093542333719615 28.547674461537266 23.23556331710275 52 19.487133313857495 26.337015390016667 34.579312122804026 19.596539173321812 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3863.0 1 4322.5 2 4782.0 3 8306.0 4 11830.0 5 8264.0 6 4698.0 7 4588.5 8 4479.0 9 4827.0 10 5175.0 11 5199.0 12 5223.0 13 4951.5 14 4925.0 15 5170.0 16 5010.0 17 4850.0 18 5147.0 19 5444.0 20 6094.5 21 6745.0 22 7432.0 23 8119.0 24 9091.5 25 10064.0 26 12402.0 27 14740.0 28 19283.5 29 23827.0 30 28195.0 31 32563.0 32 33072.0 33 33581.0 34 43781.5 35 53982.0 36 55700.0 37 57418.0 38 64399.5 39 87855.5 40 104330.0 41 122248.0 42 140166.0 43 152761.0 44 165356.0 45 160853.5 46 156351.0 47 146996.0 48 137641.0 49 126586.5 50 115532.0 51 104779.5 52 94027.0 53 80557.0 54 67087.0 55 58473.5 56 49860.0 57 43434.5 58 37009.0 59 33124.0 60 29239.0 61 26773.5 62 24308.0 63 20314.0 64 13479.0 65 10638.0 66 9483.0 67 8328.0 68 6531.5 69 4735.0 70 4092.0 71 3449.0 72 3063.0 73 2677.0 74 2231.0 75 1785.0 76 1480.5 77 1176.0 78 874.0 79 572.0 80 458.5 81 345.0 82 240.5 83 136.0 84 92.5 85 49.0 86 47.0 87 45.0 88 24.0 89 5.0 90 7.0 91 5.0 92 3.0 93 3.5 94 4.0 95 2.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1543793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.085009262231676 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25463535728935 12.104715067478665 2 9.875278643058353 3.176878968814246 3 3.389207662950163 1.6354630995053985 4 1.674627721987252 1.0774560967581952 5 1.04496447901918 0.8404131661863306 6 0.7016377686069515 0.6771510004064628 7 0.5067155785344111 0.5705367341830159 8 0.3970384457007453 0.5109093661246846 9 0.3252355745408295 0.4708275506006835 >10 4.108134790706393 16.116806316627237 >50 1.1594434513640042 13.21127216974976 >100 1.5525438154095585 47.83516844297665 >500 0.00810516217911542 0.7741466125969447 >1k 0.002026290544778855 0.5819136755349382 >5k 4.05258108955771E-4 0.4163417324567772 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6387 0.41372126962617395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3240 0.20987269666334799 No Hit CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT 2161 0.13997990663256019 TruSeq Adapter, Index 23 (96% over 25bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.477552366152716E-5 0.0 0.0 0.09483136664047577 0.0 2 6.477552366152716E-5 0.0 0.0 0.35814387032458367 0.0 3 6.477552366152716E-5 0.0 0.0 0.5162609235823715 0.0 4 6.477552366152716E-5 0.0 0.0 0.6870092039541571 0.0 5 6.477552366152716E-5 0.0 0.0 1.1857159606242547 0.0 6 6.477552366152716E-5 0.0 0.0 1.5450905658984073 0.0 7 6.477552366152716E-5 0.0 0.0 1.7769221650830131 0.0 8 6.477552366152716E-5 0.0 0.0 2.2471276913420386 0.0 9 6.477552366152716E-5 0.0 0.0 2.4239000954143464 0.0 10 6.477552366152716E-5 0.0 0.0 2.8045858479731414 0.0 11 6.477552366152716E-5 0.0 0.0 3.321494526792128 0.0 12 6.477552366152716E-5 0.0 0.0 3.7303576321436878 0.0 13 6.477552366152716E-5 0.0 0.0 3.892102114726521 0.0 14 6.477552366152716E-5 0.0 0.0 3.9492989021196494 0.0 15 6.477552366152716E-5 0.0 0.0 4.054300025974985 0.0 16 6.477552366152716E-5 0.0 0.0 4.280301828030053 0.0 17 6.477552366152716E-5 0.0 0.0 4.541347188386008 0.0 18 6.477552366152716E-5 0.0 0.0 4.911798408206281 0.0 19 6.477552366152716E-5 0.0 0.0 5.088570812278589 0.0 20 6.477552366152716E-5 0.0 0.0 5.287690772014123 0.0 21 6.477552366152716E-5 0.0 0.0 5.5540477253103235 0.0 22 6.477552366152716E-5 0.0 0.0 5.820210352035539 0.0 23 6.477552366152716E-5 0.0 0.0 6.127505436285824 0.0 24 6.477552366152716E-5 0.0 0.0 6.353701564911876 0.0 25 6.477552366152716E-5 0.0 0.0 6.5459553191392885 0.0 26 6.477552366152716E-5 0.0 0.0 6.733027031473779 0.0 27 6.477552366152716E-5 0.0 0.0 6.899629678331227 0.0 28 1.2955104732305432E-4 0.0 0.0 7.091818657034978 0.0 29 1.2955104732305432E-4 0.0 0.0 7.293918290858943 0.0 30 1.2955104732305432E-4 0.0 0.0 7.525296461377918 0.0 31 1.2955104732305432E-4 0.0 0.0 7.753241529142832 0.0 32 1.2955104732305432E-4 0.0 0.0 7.974061289304978 0.0 33 1.2955104732305432E-4 0.0 0.0 8.186265904820141 0.0 34 1.2955104732305432E-4 0.0 0.0 8.395037417581243 0.0 35 1.2955104732305432E-4 0.0 0.0 8.658220370218029 0.0 36 1.2955104732305432E-4 0.0 0.0 8.898343236431309 0.0 37 1.2955104732305432E-4 0.0 0.0 9.123502956678777 0.0 38 1.2955104732305432E-4 0.0 0.0 9.347561493024 0.0 39 1.2955104732305432E-4 0.0 0.0 9.57744982649876 0.0 40 1.2955104732305432E-4 0.0 0.0 9.81452824309995 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTACG 35 1.019871E-7 46.000004 1 TTATCCG 35 1.019871E-7 46.000004 1 CCGTATT 35 1.019871E-7 46.000004 41 GTATACG 110 0.0 46.000004 1 ACGCCGT 20 6.312132E-4 46.0 18 CGGTTCA 25 3.4177043E-5 46.0 22 CACGCTA 30 1.8618211E-6 46.0 26 ATAGCGC 20 6.312132E-4 46.0 12 GCCGGAT 20 6.312132E-4 46.0 19 ATGTACG 80 0.0 46.0 1 CGTTAGT 20 6.312132E-4 46.0 12 AATGCGC 25 3.4177043E-5 46.0 34 CCCGTAC 30 1.8618211E-6 46.0 33 CGTAGTC 20 6.312132E-4 46.0 20 GGGCGTA 45 3.110472E-10 46.0 7 ACGGATC 25 3.4177043E-5 46.0 13 TATTACG 25 3.4177043E-5 46.0 1 AGCGATA 30 1.8618211E-6 46.0 41 TGCTCGA 30 1.8618211E-6 46.0 32 CGTAAAT 20 6.312132E-4 46.0 14 >>END_MODULE