FastQCFastQC Report
Thu 26 May 2016
SRR1544747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544747_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1201583
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.46372160724644074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30810.256411750166239No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT16690.13890010095016325Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT13860.115347836978386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT3400.046.00000425
AACGTAT405.6115823E-946.010
AACCGGC206.3115696E-446.01
TCGCATA206.3115696E-446.029
ATCTCGA206.3115696E-446.010
CGTATTA301.8614737E-646.025
CATGCGT301.8614737E-646.036
CTATCGG301.8614737E-646.02
GCGAAGT206.3115696E-446.018
GACCGAA301.8614737E-646.014
GCACGTT301.8614737E-646.031
AAGCGTA253.4172495E-546.040
TGAGTCG301.8614737E-646.01
CCTAACG206.3115696E-446.019
ATCGTTA206.3115696E-446.043
CGTTATG405.6115823E-946.041
CCCGTCA301.8614737E-646.028
CCCGTAT301.8614737E-646.023
CCCCGTA301.8614737E-646.022
CCCGTAG206.3115696E-446.042
Produced by FastQC (version 0.11.5)