##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544747_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1201583
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.699294181092775	33.0	31.0	34.0	31.0	34.0
2	33.10531523831479	34.0	33.0	34.0	31.0	34.0
3	33.13209324699168	34.0	33.0	34.0	31.0	34.0
4	36.45725596983313	37.0	37.0	37.0	35.0	37.0
5	36.454855802720246	37.0	37.0	37.0	35.0	37.0
6	36.524821006954994	37.0	37.0	37.0	35.0	37.0
7	36.624461231558705	37.0	37.0	37.0	35.0	37.0
8	36.624839898700294	37.0	37.0	37.0	35.0	37.0
9	38.33896867715339	39.0	39.0	39.0	37.0	39.0
10	38.234419927712025	39.0	39.0	39.0	37.0	39.0
11	38.05962717515145	39.0	39.0	39.0	35.0	39.0
12	37.30019565856042	39.0	35.0	39.0	35.0	39.0
13	37.15102410736503	39.0	35.0	39.0	35.0	39.0
14	38.28701720979741	40.0	35.0	41.0	35.0	41.0
15	38.3282136980966	40.0	35.0	41.0	35.0	41.0
16	38.40911114754453	40.0	35.0	41.0	35.0	41.0
17	38.37742960744285	40.0	35.0	41.0	35.0	41.0
18	38.40400954407644	40.0	36.0	41.0	35.0	41.0
19	38.39718438093748	40.0	36.0	41.0	35.0	41.0
20	38.2451374561724	40.0	35.0	41.0	35.0	41.0
21	38.16532024837235	40.0	35.0	41.0	35.0	41.0
22	38.08979154998032	40.0	35.0	41.0	35.0	41.0
23	38.02330509003539	40.0	35.0	41.0	35.0	41.0
24	37.97753463555993	40.0	35.0	41.0	35.0	41.0
25	37.96232553223539	40.0	35.0	41.0	35.0	41.0
26	37.879938381285356	40.0	35.0	41.0	35.0	41.0
27	37.85492138287576	40.0	35.0	41.0	35.0	41.0
28	37.85328104675249	40.0	35.0	41.0	35.0	41.0
29	37.83813685779509	40.0	35.0	41.0	35.0	41.0
30	37.80631134095606	40.0	35.0	41.0	35.0	41.0
31	37.691596002939455	40.0	35.0	41.0	35.0	41.0
32	37.560942523321316	40.0	35.0	41.0	35.0	41.0
33	37.43805962634291	40.0	35.0	41.0	34.0	41.0
34	37.32021341846547	40.0	35.0	41.0	34.0	41.0
35	37.19363206703157	40.0	35.0	41.0	34.0	41.0
36	37.105093863678164	39.0	35.0	41.0	34.0	41.0
37	37.03962356324948	39.0	35.0	41.0	34.0	41.0
38	36.926651758555174	39.0	35.0	41.0	33.0	41.0
39	36.93227018025388	39.0	35.0	41.0	33.0	41.0
40	36.833183392241736	39.0	35.0	41.0	33.0	41.0
41	36.80510543175128	39.0	35.0	41.0	33.0	41.0
42	36.80287836961741	39.0	35.0	41.0	33.0	41.0
43	36.7350095665468	39.0	35.0	41.0	33.0	41.0
44	36.63610254139747	38.0	35.0	41.0	33.0	41.0
45	36.56843513931206	38.0	35.0	41.0	33.0	41.0
46	36.531565443252774	38.0	35.0	41.0	33.0	41.0
47	36.46373741972049	38.0	35.0	41.0	33.0	41.0
48	36.370493756985574	38.0	35.0	41.0	33.0	41.0
49	36.319468567714424	37.0	35.0	40.0	33.0	41.0
50	36.19429535870597	37.0	35.0	40.0	33.0	41.0
51	36.10114573857986	37.0	35.0	40.0	32.0	41.0
52	35.8756340594033	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	4.0
14	7.0
15	17.0
16	44.0
17	78.0
18	146.0
19	320.0
20	569.0
21	877.0
22	1400.0
23	2257.0
24	3739.0
25	6182.0
26	8836.0
27	10122.0
28	10250.0
29	10540.0
30	11542.0
31	13200.0
32	16689.0
33	23403.0
34	63020.0
35	267812.0
36	50263.0
37	60146.0
38	116404.0
39	521506.0
40	2204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.788593879906756	23.65612695918634	28.884896008016092	13.67038315289081
2	32.02883196583174	24.71514660244028	30.361864307334574	12.894157124393404
3	28.393044841679682	26.340336040040512	31.66040132059125	13.606217797688549
4	25.644753629170854	27.626555968251882	32.025752694570414	14.702937708006855
5	23.82889904401111	33.126966676459304	28.739587693900464	14.304546585629124
6	22.2573055710675	41.88291611981861	25.488293359676362	10.371484949437534
7	88.38598748484291	4.05498413343065	5.898219265751929	1.6608091159745104
8	88.99942825422796	3.139275439149855	5.997088840304831	1.8642074663173496
9	84.41023216873074	5.42392826795985	7.38284413144993	2.782995431859472
10	47.762826205097774	28.56789751519454	12.955243208334338	10.714033071373347
11	44.72058942245355	20.3629711805177	21.735327480498643	13.181111916530112
12	41.428765220546566	20.380115231324012	24.653561177213724	13.5375583709157
13	21.85392103583356	39.763961374287085	23.905298260711078	14.476819329168272
14	14.185120794818168	42.50909009198699	27.70553511492756	15.600253998267286
15	13.8171062673157	24.40563822890304	47.22694978207914	14.550305721702122
16	15.626552639309976	19.687861762358487	46.305581886561306	18.380003711770225
17	15.801904654110452	19.909569293174087	30.511333798830375	33.77719225388508
18	19.115200531299127	23.322150862653682	37.6891151089854	19.873533497061793
19	27.38795405727278	24.112025552958055	27.977176774305228	20.522843615463934
20	29.850205936668544	23.20255862474752	28.861426967591918	18.085808470992013
21	21.100831153569917	28.292261125531905	30.257751649282653	20.349156071615525
22	20.64568157172663	24.25317268969351	26.759616272866708	28.341529465713144
23	16.744328107171956	29.846876994764404	28.215945132379538	25.1928497656841
24	16.69763969696642	23.802350732325607	41.986196542394495	17.513813028313482
25	16.4080217513064	26.455850324114106	37.317355521840774	19.81877240273872
26	16.714617300677524	35.09179141182923	28.475020036069086	19.718571251424162
27	16.446221359656388	36.85330102040392	28.861343744044316	17.83913387589538
28	14.856818047525639	30.445171078485632	36.85463259716557	17.843378276823156
29	15.240728272620368	26.114966673130365	35.7730593725111	22.871245681738174
30	18.089137412896157	33.48915555562953	30.62951123642728	17.792195795047032
31	27.79857904114822	29.771892578373695	25.827346092612828	16.602182287865258
32	29.377579409828535	25.6723838469752	28.784861303796745	16.165175439399526
33	28.506145642872777	28.55175214695947	23.71704659603207	19.225055614135687
34	19.524327491317703	28.518213057275275	26.458513477637418	25.498945973769604
35	19.4760578337077	26.912248259171445	31.821855002941952	21.789838904178904
36	29.764485682636987	27.26594833648612	26.304466691023425	16.66509928985347
37	19.925215320123538	35.9947669033267	28.80283759007909	15.277180186470682
38	18.974552735849294	34.68566049952438	25.56802151828047	20.77176524634586
39	19.478304869492995	34.40361589669628	27.753721548989958	18.364357684820774
40	24.966564939750313	26.049552964714046	27.882218706489688	21.10166338904595
41	16.546588958066152	25.439441137233132	29.821909930483372	28.192059974217344
42	20.22240660861547	24.94725707670631	27.721181141877004	27.109155172801213
43	20.130194917870842	24.29961142925624	29.821077695007336	25.74911595786558
44	16.732760034055076	29.408205675346604	32.00428101928872	21.854753271309598
45	15.970515561555048	39.05697733739576	25.246612177435935	19.725894923613264
46	20.796815534174502	34.221189880349506	27.005791526677726	17.97620305879827
47	20.082840719284476	29.565498180317128	27.4993071639662	22.85235393643219
48	20.859149971329487	26.178965581237417	33.37855146086454	19.583332986568553
49	21.522774539919425	26.215833612825744	34.246739509463765	18.014652337791066
50	20.459427272190105	33.68989075244906	28.230259582567328	17.620422392793504
51	18.027968105407616	33.51636965569586	26.718836734541018	21.736825504355505
52	19.53614523507739	27.535842301364116	34.50107067093992	18.42694179261857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3657.0
1	3696.0
2	3735.0
3	6652.5
4	9570.0
5	6451.5
6	3333.0
7	3536.5
8	3740.0
9	3876.0
10	4012.0
11	4085.5
12	4159.0
13	4230.5
14	4161.5
15	4021.0
16	3940.5
17	3860.0
18	4061.5
19	4263.0
20	4267.5
21	4272.0
22	5168.0
23	6064.0
24	7039.0
25	8014.0
26	9915.0
27	11816.0
28	12928.5
29	14041.0
30	19132.5
31	24224.0
32	24552.5
33	24881.0
34	31391.5
35	37902.0
36	41408.5
37	44915.0
38	48504.5
39	67481.5
40	82869.0
41	106052.0
42	129235.0
43	133465.0
44	137695.0
45	133347.5
46	129000.0
47	113908.5
48	98817.0
49	90360.0
50	81903.0
51	69811.0
52	57719.0
53	52940.5
54	48162.0
55	43165.5
56	38169.0
57	33968.0
58	29767.0
59	28074.0
60	26381.0
61	23767.5
62	21154.0
63	17622.5
64	11763.5
65	9436.0
66	8165.0
67	6894.0
68	5474.5
69	4055.0
70	3773.5
71	3492.0
72	2974.5
73	2457.0
74	2023.5
75	1590.0
76	1227.5
77	865.0
78	627.0
79	389.0
80	349.5
81	310.0
82	234.5
83	159.0
84	104.5
85	50.0
86	38.0
87	26.0
88	18.0
89	10.0
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1201583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.706893075570688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82259506498764	11.909373933980547
2	9.411114080553693	2.9563872517050918
3	3.196085239692628	1.506017073607855
4	1.5749302013005193	0.9894904109325711
5	0.9243116860611266	0.7259032410731288
6	0.6369562235558534	0.6002761978326653
7	0.4566941056945382	0.5021271840471244
8	0.37650686762186697	0.47310024895637703
9	0.3190272402326556	0.4509834075475853
>10	4.506346799887843	17.42057660030238
>50	1.1310673602177022	12.447441332358594
>100	1.6331717208798169	48.177302691644435
>500	0.009061331349529009	0.8608172815030412
>1k	0.0015990584734462958	0.5137108382904747
>5k	5.330194911487652E-4	0.4664923062181429
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5572	0.46372160724644074	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3081	0.256411750166239	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	1669	0.13890010095016325	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1386	0.115347836978386	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1433941725207497	0.0
2	0.0	0.0	0.0	0.41253912547031707	0.0
3	0.0	0.0	0.0	0.5511063322300666	0.0
4	0.0	0.0	0.0	0.703655094987196	0.0
5	0.0	0.0	0.0	1.1384981312152385	0.0
6	0.0	0.0	0.0	1.4430130918962736	0.0
7	0.0	0.0	0.0	1.64200059421613	0.0
8	0.0	0.0	0.0	2.0689373934218445	0.0
9	0.0	0.0	0.0	2.2070884824435764	0.0
10	0.0	0.0	0.0	2.4921291329854034	0.0
11	0.0	0.0	0.0	2.887607431197013	0.0
12	0.0	0.0	0.0	3.2146759732785832	0.0
13	0.0	0.0	0.0	3.341508659826246	0.0
14	0.0	0.0	0.0	3.3905273293646796	0.0
15	0.0	0.0	0.0	3.4749160066345812	0.0
16	0.0	0.0	0.0	3.66624694257492	0.0
17	0.0	0.0	0.0	3.9026850413163303	0.0
18	0.0	0.0	0.0	4.215938474495728	0.0
19	0.0	0.0	0.0	4.3540895635174595	0.0
20	8.322354760345311E-5	0.0	0.0	4.51629225779659	0.0
21	8.322354760345311E-5	0.0	0.0	4.735336635088879	0.0
22	8.322354760345311E-5	0.0	0.0	4.961704684570271	0.0
23	8.322354760345311E-5	0.0	0.0	5.208379279666906	0.0
24	2.496706428103593E-4	0.0	0.0	5.40079212172609	0.0
25	2.496706428103593E-4	0.0	0.0	5.55841752088703	0.0
26	3.3289419041381246E-4	0.0	0.0	5.721036332904177	0.0
27	3.3289419041381246E-4	0.0	0.0	5.869423918281134	0.0
28	3.3289419041381246E-4	0.0	0.0	6.03587101348804	0.0
29	3.3289419041381246E-4	0.0	0.0	6.205397379956274	0.0
30	3.3289419041381246E-4	0.0	0.0	6.41786709698789	0.0
31	3.3289419041381246E-4	0.0	0.0	6.617603611236178	0.0
32	3.3289419041381246E-4	0.0	0.0	6.803774687225102	0.0
33	3.3289419041381246E-4	0.0	0.0	6.987532280333527	0.0
34	3.3289419041381246E-4	0.0	0.0	7.184855311701314	0.0
35	3.3289419041381246E-4	0.0	0.0	7.446593368914174	0.0
36	3.3289419041381246E-4	0.0	0.0	7.659479203683807	0.0
37	3.3289419041381246E-4	0.0	0.0	7.856302893765974	0.0
38	3.3289419041381246E-4	0.0	0.0	8.052127901276899	0.0
39	3.3289419041381246E-4	0.0	0.0	8.247453567502204	0.0
40	3.3289419041381246E-4	0.0	0.0	8.476151876316493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	340	0.0	46.000004	25
AACGTAT	40	5.6115823E-9	46.0	10
AACCGGC	20	6.3115696E-4	46.0	1
TCGCATA	20	6.3115696E-4	46.0	29
ATCTCGA	20	6.3115696E-4	46.0	10
CGTATTA	30	1.8614737E-6	46.0	25
CATGCGT	30	1.8614737E-6	46.0	36
CTATCGG	30	1.8614737E-6	46.0	2
GCGAAGT	20	6.3115696E-4	46.0	18
GACCGAA	30	1.8614737E-6	46.0	14
GCACGTT	30	1.8614737E-6	46.0	31
AAGCGTA	25	3.4172495E-5	46.0	40
TGAGTCG	30	1.8614737E-6	46.0	1
CCTAACG	20	6.3115696E-4	46.0	19
ATCGTTA	20	6.3115696E-4	46.0	43
CGTTATG	40	5.6115823E-9	46.0	41
CCCGTCA	30	1.8614737E-6	46.0	28
CCCGTAT	30	1.8614737E-6	46.0	23
CCCCGTA	30	1.8614737E-6	46.0	22
CCCGTAG	20	6.3115696E-4	46.0	42
>>END_MODULE