FastQCFastQC Report
Thu 26 May 2016
SRR1544746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544746_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1458553
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68890.47231742692929224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43650.29926920722112943No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT22770.15611362768442422Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT18480.1267009152221414Illumina Single End Adapter 2 (95% over 21bp)
TGTATGGGGGCTCTCTCTCTTGCTTCTTGCTCTCTTGCTTCTTGCTCTCTTG15520.10640682923417936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC351.0198164E-746.00000432
CGACGGT1150.046.00000428
CGTTCTA700.046.00000430
GTCGTAA351.0198164E-746.00000432
CGTAATC351.0198164E-746.00000434
CGGTCTA1150.046.00000431
ACGTAAG1400.046.0000041
CGGAGAT351.0198164E-746.0000046
CTATGCG453.110472E-1046.01
AACCGCA453.110472E-1046.026
TCGTTAC453.110472E-1046.025
GATCGAC650.046.09
GTTTCGA206.3120166E-446.043
ACGTTAC206.3120166E-446.038
CGGTTAT253.417612E-546.016
GACCGGT253.417612E-546.026
CGACCCA253.417612E-546.044
GTTAGCG206.3120166E-446.01
GAATACG206.3120166E-446.09
TATGCGC405.6134013E-946.021
Produced by FastQC (version 0.11.5)