##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544746_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1458553
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.682946728709894	33.0	31.0	34.0	31.0	34.0
2	33.08823128127672	34.0	33.0	34.0	31.0	34.0
3	33.138535246919375	34.0	33.0	34.0	31.0	34.0
4	36.45223039546729	37.0	37.0	37.0	35.0	37.0
5	36.44958530817872	37.0	37.0	37.0	35.0	37.0
6	36.519935854233616	37.0	37.0	37.0	35.0	37.0
7	36.63684624418859	37.0	37.0	37.0	35.0	37.0
8	36.6310768275133	37.0	37.0	37.0	35.0	37.0
9	38.36835068729076	39.0	39.0	39.0	37.0	39.0
10	38.23556428871628	39.0	39.0	39.0	37.0	39.0
11	38.07185683345069	39.0	39.0	39.0	35.0	39.0
12	37.46260026204053	39.0	35.0	39.0	35.0	39.0
13	37.34904868043876	39.0	35.0	39.0	35.0	39.0
14	38.57237207012704	40.0	35.0	41.0	35.0	41.0
15	38.58029567660551	40.0	35.0	41.0	35.0	41.0
16	38.66023312145668	41.0	35.0	41.0	35.0	41.0
17	38.61139979143713	40.0	35.0	41.0	35.0	41.0
18	38.63312406199843	40.0	36.0	41.0	35.0	41.0
19	38.626283035309655	40.0	36.0	41.0	35.0	41.0
20	38.52788894198565	40.0	35.0	41.0	35.0	41.0
21	38.463324952881386	40.0	35.0	41.0	35.0	41.0
22	38.39121444335585	40.0	35.0	41.0	35.0	41.0
23	38.313459298359405	40.0	35.0	41.0	35.0	41.0
24	38.27246318783068	40.0	35.0	41.0	35.0	41.0
25	38.26667525965803	40.0	35.0	41.0	35.0	41.0
26	38.21739011198085	40.0	35.0	41.0	35.0	41.0
27	38.18321308858849	40.0	35.0	41.0	35.0	41.0
28	38.163534681290294	40.0	35.0	41.0	35.0	41.0
29	38.14721096867923	40.0	36.0	41.0	35.0	41.0
30	38.08864744716168	40.0	36.0	41.0	35.0	41.0
31	37.99934661270451	40.0	35.0	41.0	35.0	41.0
32	37.89231930550347	40.0	35.0	41.0	35.0	41.0
33	37.765891263464546	40.0	35.0	41.0	35.0	41.0
34	37.648767648484494	40.0	35.0	41.0	34.0	41.0
35	37.54358600613073	40.0	35.0	41.0	34.0	41.0
36	37.47376612299999	40.0	35.0	41.0	34.0	41.0
37	37.45376136485956	40.0	35.0	41.0	34.0	41.0
38	37.34526959253452	40.0	35.0	41.0	34.0	41.0
39	37.31696071380334	40.0	35.0	41.0	34.0	41.0
40	37.244434038392846	40.0	35.0	41.0	33.0	41.0
41	37.20737402069037	40.0	35.0	41.0	34.0	41.0
42	37.191085274240976	40.0	35.0	41.0	34.0	41.0
43	37.13383332659149	40.0	35.0	41.0	33.0	41.0
44	37.06362675884935	40.0	35.0	41.0	33.0	41.0
45	37.00660037722318	39.0	35.0	41.0	33.0	41.0
46	36.96789009381215	39.0	35.0	41.0	33.0	41.0
47	36.89535176301444	39.0	35.0	41.0	33.0	41.0
48	36.834243253416226	39.0	35.0	41.0	33.0	41.0
49	36.79958355987064	39.0	35.0	41.0	33.0	41.0
50	36.71345230512707	39.0	35.0	41.0	33.0	41.0
51	36.637321372620676	39.0	35.0	41.0	33.0	41.0
52	36.44394478637389	38.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	8.0
14	5.0
15	21.0
16	38.0
17	84.0
18	157.0
19	316.0
20	637.0
21	1065.0
22	1585.0
23	2720.0
24	4342.0
25	7113.0
26	9707.0
27	11187.0
28	11475.0
29	11885.0
30	13071.0
31	15113.0
32	19328.0
33	26728.0
34	66868.0
35	267753.0
36	57434.0
37	70722.0
38	144074.0
39	712408.0
40	2704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.498752530761653	24.858884113227287	29.603723690534384	14.038639665476675
2	28.433522813363656	26.39684673782852	31.270375502295767	13.899254946512057
3	28.03031497655553	25.922541038961217	32.618149631861165	13.428994352622084
4	25.37830301675702	28.86079559673183	31.157318246234457	14.6035831402767
5	22.636476014241513	34.68163309800878	28.59800089540798	14.083889992341724
6	21.540663931992874	43.32986185623697	24.97838611281181	10.15108809895835
7	89.73599176718295	3.4146170896772348	5.3178047009604725	1.531586442179338
8	89.81888213866756	3.0647497896888214	5.190760980231778	1.9256070914118308
9	85.27547507701125	5.38177220848334	6.771917098658739	2.5708356158466645
10	40.53668258883976	37.7849142266342	11.435100404304814	10.243302780221219
11	39.186166015221936	21.327096101410095	25.52694348439858	13.959794398969388
12	38.368163515484184	20.902154395486487	25.51515097497314	15.21453111405619
13	21.48451239001942	36.34773642095968	26.215228380456523	15.952522808564378
14	15.55486842096242	39.64593676061137	28.121021313589566	16.678173504836643
15	14.17884711765702	26.718878230684794	43.915099417024955	15.18717523463323
16	18.068318395012042	23.031662202196287	42.03954192956992	16.860477473221746
17	16.890850041102382	22.123707537538916	30.147001857320234	30.838440564038468
18	18.180347234553697	24.566265332833296	37.60507845789628	19.64830897471672
19	24.415910837658966	24.998337393293216	29.797340240635755	20.788411528412063
20	26.994013930244563	24.85408483613554	30.27493687236597	17.87696436125393
21	20.92484812002032	28.787092412822844	31.734122791561227	18.553936675595608
22	21.79454569014633	23.92665881870594	28.047798057389755	26.23099743375798
23	16.846353886351746	29.854588760230172	28.454708193668655	24.844349159749424
24	17.01439714566423	24.18198035998692	41.2968195190713	17.50680297527755
25	16.280861922741238	26.773452867328096	36.57679906043867	20.368886149491995
26	15.934971166628845	34.36049289946954	29.246040424996554	20.45849550890506
27	16.285386955427743	35.58780517403207	29.93549085977678	18.19131701076341
28	14.712389608056753	30.27020615637553	36.654135982717115	18.3632682528506
29	16.050702305641277	25.210534001849776	34.85714951736413	23.88161417514482
30	16.974974512410586	33.347022699895035	31.651438103380542	18.026564684313836
31	25.108789327504727	29.0095046254747	27.191195657614088	18.690510389406487
32	26.339392534930166	26.460540000946143	29.39824606990627	17.80182139421742
33	26.329793980746675	27.72686354215445	27.250089643639964	18.693252833458914
34	19.255453864206512	27.499172124701676	28.113822397951942	25.131551613139873
35	18.930474243993874	25.677777907282078	32.7467016968187	22.645046151905348
36	26.545144399963526	26.42002039007153	28.85585919743746	18.178976012527485
37	18.64279186289425	32.39320065846082	31.489565343186023	17.474442135458908
38	18.41571749535327	32.84097321112088	27.10583708648229	21.637472207043558
39	19.534977474250166	32.51030301949947	29.36369127484569	18.591028231404685
40	24.540349236537857	26.306003278591866	28.19102219802777	20.962625286842506
41	17.803398299547567	25.59762997984989	30.874298020023954	25.724673700578588
42	19.369196731280933	25.205323358150167	29.471195081700834	25.95428482886806
43	19.344994662518264	26.173543230859625	30.12067439441693	24.36078771220518
44	16.936511734575294	29.212171240949075	30.77131924585531	23.079997778620317
45	16.44088353320037	35.928690969748786	27.193183929552095	20.43724156749875
46	20.526645243607877	32.865586646491415	27.96367358608155	18.644094523819156
47	18.498950672344442	28.84331251589761	29.296432834459907	23.361303977298046
48	19.794069876103233	26.119242838621563	34.05800132048682	20.02868596478839
49	20.909970361035903	25.137790673359145	34.65297455766091	19.29926440794404
50	19.215825547648937	32.04991522419823	29.793158013455802	18.94110121469703
51	18.228682810977727	30.977139671989978	28.306136287128407	22.488041229903885
52	18.56518069621056	27.526596565225947	35.46446375277416	18.44375898578934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5241.0
1	5049.5
2	4858.0
3	8293.5
4	11729.0
5	7883.5
6	4038.0
7	4073.0
8	4108.0
9	4348.5
10	4589.0
11	4607.0
12	4625.0
13	4850.0
14	4793.0
15	4511.0
16	4483.0
17	4455.0
18	4708.5
19	4962.0
20	5281.0
21	5600.0
22	7350.0
23	9100.0
24	9616.5
25	10133.0
26	13462.5
27	16792.0
28	19386.5
29	21981.0
30	26931.0
31	31881.0
32	34204.0
33	36527.0
34	45501.5
35	54476.0
36	58276.0
37	62076.0
38	68313.0
39	91987.5
40	109425.0
41	121958.5
42	134492.0
43	143231.5
44	151971.0
45	142530.0
46	133089.0
47	126112.0
48	119135.0
49	114625.5
50	110116.0
51	103203.0
52	96290.0
53	82077.0
54	67864.0
55	56521.5
56	45179.0
57	38611.0
58	32043.0
59	28648.5
60	25254.0
61	21569.0
62	17884.0
63	14912.5
64	9786.5
65	7632.0
66	6480.0
67	5328.0
68	4398.0
69	3468.0
70	2886.5
71	2305.0
72	1886.5
73	1468.0
74	1280.0
75	1092.0
76	893.5
77	695.0
78	487.0
79	279.0
80	218.5
81	158.0
82	109.5
83	61.0
84	45.0
85	29.0
86	31.0
87	33.0
88	22.5
89	7.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1458553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.110510980323273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68281119271886	12.031782497617067
2	10.26229991784411	3.3066179101959627
3	3.618981253746735	1.7491091167821258
4	1.7143447897295696	1.104758822359929
5	1.066392291616511	0.8590062361709947
6	0.6963002846946905	0.6730652029305619
7	0.5097093019499231	0.5748174114086003
8	0.40557247376867694	0.5227183833573713
9	0.32182658439720024	0.4666311649521926
>10	4.02289139526136	15.533532381299198
>50	1.1243153888537591	12.825412893335233
>100	1.5638555118998458	48.1399954995976
>500	0.007703721733496778	0.8638118192949099
>1k	0.002567907244498926	0.8737406907810583
>5k	4.2798454074982097E-4	0.47499996991719623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6889	0.47231742692929224	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4365	0.29926920722112943	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	2277	0.15611362768442422	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1848	0.1267009152221414	Illumina Single End Adapter 2 (95% over 21bp)
TGTATGGGGGCTCTCTCTCTTGCTTCTTGCTCTCTTGCTTCTTGCTCTCTTG	1552	0.10640682923417936	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16262693230893907	0.0
2	0.0	0.0	0.0	0.48404137525341895	0.0
3	0.0	0.0	0.0	0.6561297395432323	0.0
4	0.0	0.0	0.0	0.8816272017540672	0.0
5	6.856110131068258E-5	0.0	0.0	1.4678246179604033	0.0
6	6.856110131068258E-5	0.0	0.0	1.8807681311546445	0.0
7	6.856110131068258E-5	0.0	0.0	2.157754980449802	0.0
8	6.856110131068258E-5	0.0	0.0	2.7287318321651663	0.0
9	6.856110131068258E-5	0.0	0.0	2.9104187506384753	0.0
10	6.856110131068258E-5	0.0	0.0	3.288053296657715	0.0
11	6.856110131068258E-5	0.0	0.0	3.823172692387592	0.0
12	6.856110131068258E-5	0.0	0.0	4.241943899193242	0.0
13	6.856110131068258E-5	0.0	0.0	4.401828387449753	0.0
14	6.856110131068258E-5	0.0	0.0	4.455785974181261	0.0
15	6.856110131068258E-5	0.0	0.0	4.556845037513207	0.0
16	6.856110131068258E-5	0.0	0.0	4.784810699371226	0.0
17	1.3712220262136515E-4	0.0	0.0	5.05446151082614	0.0
18	1.3712220262136515E-4	0.0	0.0	5.4013806834581946	0.0
19	3.428055065534129E-4	0.0	0.0	5.586907023604901	0.0
20	3.428055065534129E-4	0.0	0.0	5.7809349403141335	0.0
21	3.428055065534129E-4	0.0	0.0	6.0558649565699705	0.0
22	4.7992770917477804E-4	0.0	0.0	6.330589289521876	0.0
23	4.7992770917477804E-4	0.0	0.0	6.665441708323249	0.0
24	6.856110131068258E-4	0.0	0.0	6.918912099868843	0.0
25	6.856110131068258E-4	0.0	0.0	7.125486698117929	0.0
26	6.856110131068258E-4	0.0	0.0	7.341659850550512	0.0
27	6.856110131068258E-4	0.0	0.0	7.532602517700762	0.0
28	6.856110131068258E-4	0.0	0.0	7.7408225823813055	0.0
29	6.856110131068258E-4	0.0	0.0	7.961932134108257	0.0
30	6.856110131068258E-4	0.0	0.0	8.233502656399871	0.0
31	6.856110131068258E-4	0.0	0.0	8.477648738167211	0.0
32	6.856110131068258E-4	0.0	0.0	8.706094327734405	0.0
33	6.856110131068258E-4	0.0	0.0	8.932414523160968	0.0
34	6.856110131068258E-4	0.0	0.0	9.161134357133406	0.0
35	7.541721144175083E-4	0.0	0.0	9.451696304488078	0.0
36	7.541721144175083E-4	0.0	0.0	9.70592086814809	0.0
37	7.541721144175083E-4	0.0	0.0	9.949518461104944	0.0
38	7.541721144175083E-4	0.0	0.0	10.205868418905586	0.0
39	7.541721144175083E-4	0.0	0.0	10.444666734770694	0.0
40	7.541721144175083E-4	0.0	0.0	10.720213800938327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	35	1.0198164E-7	46.000004	32
CGACGGT	115	0.0	46.000004	28
CGTTCTA	70	0.0	46.000004	30
GTCGTAA	35	1.0198164E-7	46.000004	32
CGTAATC	35	1.0198164E-7	46.000004	34
CGGTCTA	115	0.0	46.000004	31
ACGTAAG	140	0.0	46.000004	1
CGGAGAT	35	1.0198164E-7	46.000004	6
CTATGCG	45	3.110472E-10	46.0	1
AACCGCA	45	3.110472E-10	46.0	26
TCGTTAC	45	3.110472E-10	46.0	25
GATCGAC	65	0.0	46.0	9
GTTTCGA	20	6.3120166E-4	46.0	43
ACGTTAC	20	6.3120166E-4	46.0	38
CGGTTAT	25	3.417612E-5	46.0	16
GACCGGT	25	3.417612E-5	46.0	26
CGACCCA	25	3.417612E-5	46.0	44
GTTAGCG	20	6.3120166E-4	46.0	1
GAATACG	20	6.3120166E-4	46.0	9
TATGCGC	40	5.6134013E-9	46.0	21
>>END_MODULE