FastQCFastQC Report
Thu 26 May 2016
SRR1544745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544745_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1520393
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79980.5260481993800287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51060.33583422180975575No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT22150.14568601670752235Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT19320.12707240825234Illumina Single End Adapter 2 (95% over 22bp)
GAGTCAGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTA17540.11536490894130662No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT206.312099E-446.00000417
ACGTTCC206.312099E-446.00000420
TTAGCCG206.312099E-446.00000411
CTATACG551.8189894E-1246.0000041
GACGTAT351.0198528E-746.00000413
TAGCGCG206.312099E-446.0000041
TCCTCGA206.312099E-446.00000426
CGCGACA351.0198528E-746.00000412
GTTCGGA206.312099E-446.00000413
CACGTTC351.0198528E-746.00000446
TTCGCTA206.312099E-446.00000436
CGATCCA206.312099E-446.00000437
ATGTTCG206.312099E-446.00000413
CGATAAG206.312099E-446.00000410
TCGAACG405.6134013E-946.0000041
GCGTAAT206.312099E-446.0000047
TCGCGCG206.312099E-446.0000041
ACGACCC206.312099E-446.00000411
ACGCGAT301.861803E-646.025
GTCGACG301.861803E-646.028
Produced by FastQC (version 0.11.5)