##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544745_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1520393 Sequences flagged as poor quality 0 Sequence length 52 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.707300020455236 33.0 31.0 34.0 31.0 34.0 2 33.119803892809294 34.0 33.0 34.0 31.0 34.0 3 33.17033030275725 34.0 33.0 34.0 31.0 34.0 4 36.478729512698365 37.0 37.0 37.0 35.0 37.0 5 36.47618411818523 37.0 37.0 37.0 35.0 37.0 6 36.54568128108982 37.0 37.0 37.0 35.0 37.0 7 36.63891046591243 37.0 37.0 37.0 35.0 37.0 8 36.63018048622955 37.0 37.0 37.0 35.0 37.0 9 38.33223186373523 39.0 39.0 39.0 37.0 39.0 10 38.24439273266846 39.0 39.0 39.0 37.0 39.0 11 38.10618373012767 39.0 39.0 39.0 35.0 39.0 12 37.54106800018153 39.0 35.0 39.0 35.0 39.0 13 37.41219474175427 39.0 35.0 39.0 35.0 39.0 14 38.66403817960225 41.0 36.0 41.0 35.0 41.0 15 38.68213744735736 41.0 36.0 41.0 35.0 41.0 16 38.75027048927481 41.0 36.0 41.0 35.0 41.0 17 38.70606547123013 40.0 36.0 41.0 35.0 41.0 18 38.71655881078116 40.0 36.0 41.0 35.0 41.0 19 38.69544584854047 40.0 36.0 41.0 35.0 41.0 20 38.577799292682876 40.0 36.0 41.0 35.0 41.0 21 38.517707592707936 40.0 35.0 41.0 35.0 41.0 22 38.45606892428471 40.0 35.0 41.0 35.0 41.0 23 38.3818216737383 40.0 35.0 41.0 35.0 41.0 24 38.33442274464563 40.0 35.0 41.0 35.0 41.0 25 38.3324877186359 40.0 35.0 41.0 35.0 41.0 26 38.280025624953545 40.0 35.0 41.0 35.0 41.0 27 38.26617723180783 40.0 35.0 41.0 35.0 41.0 28 38.218600717051444 40.0 35.0 41.0 35.0 41.0 29 38.18651822259113 40.0 36.0 41.0 35.0 41.0 30 38.141412779458996 40.0 36.0 41.0 35.0 41.0 31 38.03868341935276 40.0 35.0 41.0 35.0 41.0 32 37.92692941890682 40.0 35.0 41.0 35.0 41.0 33 37.79993396444209 40.0 35.0 41.0 35.0 41.0 34 37.67591931822891 40.0 35.0 41.0 35.0 41.0 35 37.56405876638475 40.0 35.0 41.0 34.0 41.0 36 37.507262267058586 40.0 35.0 41.0 34.0 41.0 37 37.451355669224995 40.0 35.0 41.0 34.0 41.0 38 37.334647028761644 40.0 35.0 41.0 33.0 41.0 39 37.3533152283653 40.0 35.0 41.0 34.0 41.0 40 37.29264801929501 40.0 35.0 41.0 33.0 41.0 41 37.26898900481652 40.0 35.0 41.0 34.0 41.0 42 37.24469791692017 40.0 35.0 41.0 34.0 41.0 43 37.19010347982397 40.0 35.0 41.0 34.0 41.0 44 37.08365600209946 40.0 35.0 41.0 34.0 41.0 45 37.018190691485685 40.0 35.0 41.0 33.0 41.0 46 36.96139748078293 39.0 35.0 41.0 33.0 41.0 47 36.90667610282342 39.0 35.0 41.0 33.0 41.0 48 36.84637590412479 39.0 35.0 41.0 33.0 41.0 49 36.804259161940365 39.0 35.0 41.0 33.0 41.0 50 36.69005776795868 39.0 35.0 41.0 33.0 41.0 51 36.630711270046625 39.0 35.0 41.0 33.0 41.0 52 36.40556093062781 38.0 35.0 41.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 2.0 12 2.0 13 4.0 14 12.0 15 19.0 16 36.0 17 80.0 18 177.0 19 364.0 20 621.0 21 1122.0 22 1821.0 23 2920.0 24 4808.0 25 7776.0 26 10718.0 27 12328.0 28 12053.0 29 12056.0 30 12855.0 31 15406.0 32 19355.0 33 26743.0 34 66895.0 35 267097.0 36 58576.0 37 76497.0 38 154788.0 39 752437.0 40 2823.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.34585202641686 23.45340974340187 29.154501500598855 14.046236729582418 2 28.58241257359117 26.85858195874356 30.53328974811118 14.02571571955409 3 28.754407577514495 25.246301449690968 32.17641754467431 13.82287342812023 4 24.31200354118968 28.865694593437354 31.535596388565324 15.286705476807642 5 22.78141243744216 33.99154034516076 28.796633501995867 14.430413715401217 6 22.011019519295342 42.71244342745592 24.855152582259983 10.421384470988752 7 88.65635398216119 3.7269969014590307 6.005355194347777 1.6112939220320008 8 88.77658605373742 3.0959758430879383 6.067707494049237 2.059730609125404 9 83.32148332700822 6.052185191591911 7.502994291607498 3.123337189792376 10 40.09759318807703 36.978136573898986 12.784655020116508 10.139615217907476 11 38.809176311651 21.33606245227385 25.001825185988096 14.85293605008705 12 38.00491057246383 21.940182571216784 25.618376301390494 14.43653055492889 13 21.862965693738396 36.31482123372049 25.965260297830888 15.856952774710223 14 15.54091606578036 38.3391004825726 28.879375266789577 17.240608184857468 15 14.119836121318633 27.25999133118871 43.15206660383203 15.468105943660621 16 17.970682580096067 22.928479675978515 41.35930644247902 17.741531301446404 17 16.83222693080013 22.827387392601782 30.197521298769463 30.142864377828626 18 18.64215370631146 24.491167744129314 36.682094695253134 20.18458385430609 19 24.67539642710799 26.12278535878552 29.332152936773586 19.869665277332903 20 27.179551602776385 24.801942655616017 30.124513859245607 17.893991882361995 21 20.64288641160542 28.980401777698265 31.031581965978532 19.34512984471778 22 21.6728832611042 24.40783402712325 27.859375832432796 26.05990687933975 23 17.413852865673547 30.817361037573836 28.055706649530748 23.71307944722187 24 17.108142434225886 24.553520043830773 40.630744814005325 17.707592707938012 25 16.633133670044522 28.370362136631776 34.86887929633983 20.12762489698387 26 16.400891085397 34.68859696144352 28.0456434619207 20.864868491238777 27 15.687588669508475 36.0271982309837 29.35675183982036 18.928461259687463 28 15.152661186943112 29.728892463987926 36.41953100283939 18.698915346229562 29 15.437916380830483 26.20039687107215 35.280088766522866 23.0815979815745 30 17.884126012156067 31.85821034429914 31.754947569477103 18.502716074067692 31 24.537208471756973 29.742441592404067 27.220330532960883 18.50001940287807 32 25.84529131612682 27.76269030441471 29.296241169224007 17.09577721023446 33 25.255772685088658 28.983427311228084 26.720130913520386 19.040669090162872 34 18.79750827582079 28.639897710657703 28.897133833160243 23.66546018036126 35 18.936025093512008 27.763545346499228 31.096302074529415 22.204127485459352 36 26.413368122584096 27.55017946017905 27.391273177395583 18.645179239841276 37 18.04211148038698 33.30323146712725 31.05631241396139 17.59834463852438 38 18.680893689986732 32.65011085949488 27.918176418860124 20.750819031658263 39 19.45891621442614 32.19726741704283 29.69534850528778 18.648467863243255 40 21.887301506913015 27.79393222673348 29.832747191022317 20.48601907533118 41 16.62734569285704 26.312078521803244 31.287305321716165 25.77327046362355 42 19.08670981779053 26.133835133416163 28.792358291573294 25.98709675722001 43 19.766665592383024 26.165603235479246 30.36951630269279 23.69821486944494 44 16.301180023849096 29.702649249240164 31.85682912247031 22.13934160444043 45 16.424898036231422 36.78825145866891 26.629891087370172 20.156959417729496 46 19.74686807950313 33.63893414400093 27.33089405173531 19.283303724760636 47 18.23114155353254 29.98553663427811 29.07419331712261 22.709128495066736 48 19.448458392007854 26.394359879320678 33.75081311213614 20.40636861653533 49 19.825926586086624 26.96940856739014 33.10341470922321 20.101250137300028 50 18.62978848232003 32.97726311552342 29.496912969212563 18.896035432943982 51 17.05388014809329 31.571705473519017 28.823797531296186 22.550616847091508 52 18.320986744874514 28.4266633692736 34.22056007887434 19.03178980697754 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5893.0 1 5750.0 2 5607.0 3 9656.5 4 13706.0 5 9161.0 6 4616.0 7 4803.5 8 4991.0 9 5129.5 10 5268.0 11 5215.5 12 5163.0 13 5302.5 14 5041.5 15 4641.0 16 4761.0 17 4881.0 18 4757.5 19 4634.0 20 4862.0 21 5090.0 22 6878.0 23 8666.0 24 9058.5 25 9451.0 26 13200.5 27 16950.0 28 22226.0 29 27502.0 30 30611.0 31 33720.0 32 39415.5 33 45111.0 34 50647.5 35 56184.0 36 62938.0 37 69692.0 38 73096.5 39 87730.5 40 98960.0 41 125586.5 42 152213.0 43 160828.0 44 169443.0 45 162064.0 46 154685.0 47 139000.0 48 123315.0 49 112630.0 50 101945.0 51 87913.0 52 73881.0 53 65775.0 54 57669.0 55 50636.5 56 43604.0 57 39148.0 58 34692.0 59 32433.0 60 30174.0 61 25897.5 62 21621.0 63 18718.5 64 12782.5 65 9749.0 66 8611.5 67 7474.0 68 5862.0 69 4250.0 70 3571.5 71 2893.0 72 2357.5 73 1822.0 74 1481.5 75 1141.0 76 884.0 77 627.0 78 513.0 79 399.0 80 300.0 81 201.0 82 134.5 83 68.0 84 47.0 85 26.0 86 17.0 87 8.0 88 5.0 89 3.5 90 5.0 91 3.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1520393.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.443506667443938 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49507610516724 11.65908711189338 2 9.837070387685749 3.038377242406804 3 3.37229206529814 1.5624004498500037 4 1.6315925365798263 1.0079004086888925 5 0.9775673789702979 0.7548534167501744 6 0.637511686726652 0.5907249590721889 7 0.47364489698624873 0.512031668722555 8 0.39390335801026777 0.4866599308608101 9 0.29332732720948007 0.40770022801528033 >10 4.052479439074235 15.13242705874035 >50 1.1617816271004024 12.579815292901184 >100 1.6609110950953596 49.75984681403413 >500 0.0089894672672687 0.9134941467267224 >1k 0.002996489089089566 0.7283889498216114 >5k 8.56139739739876E-4 0.8662923215159191 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7998 0.5260481993800287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5106 0.33583422180975575 No Hit CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT 2215 0.14568601670752235 Illumina Single End Adapter 2 (95% over 23bp) CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT 1932 0.12707240825234 Illumina Single End Adapter 2 (95% over 22bp) GAGTCAGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTA 1754 0.11536490894130662 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15173708376715758 0.0 2 0.0 0.0 0.0 0.4627750851260168 0.0 3 0.0 0.0 0.0 0.6281928422453932 0.0 4 0.0 0.0 0.0 0.8245236593433408 0.0 5 0.0 0.0 0.0 1.3573464229314394 0.0 6 0.0 0.0 0.0 1.7156748288107089 0.0 7 0.0 0.0 0.0 1.950482539711772 0.0 8 6.577246803951347E-5 0.0 0.0 2.401681670462834 0.0 9 6.577246803951347E-5 0.0 0.0 2.5594040488215875 0.0 10 6.577246803951347E-5 0.0 0.0 2.8996450259899906 0.0 11 1.3154493607902694E-4 0.0 0.0 3.325455984077801 0.0 12 1.3154493607902694E-4 0.0 0.0 3.691216678845535 0.0 13 1.3154493607902694E-4 0.0 0.0 3.823353567136918 0.0 14 1.3154493607902694E-4 0.0 0.0 3.873274870378909 0.0 15 1.3154493607902694E-4 0.0 0.0 3.970289260737191 0.0 16 1.3154493607902694E-4 0.0 0.0 4.152347452270564 0.0 17 1.973174041185404E-4 0.0 0.0 4.393666637507539 0.0 18 1.973174041185404E-4 0.0 0.0 4.707532854992097 0.0 19 2.630898721580539E-4 0.0 0.0 4.862098154884954 0.0 20 2.630898721580539E-4 0.0 0.0 5.038697231571048 0.0 21 2.630898721580539E-4 0.0 0.0 5.260021586524011 0.0 22 2.630898721580539E-4 0.0 0.0 5.507326066352581 0.0 23 3.2886234019756735E-4 0.0 0.0 5.796396063386243 0.0 24 4.604072762765943E-4 0.0 0.0 6.0172600110629295 0.0 25 4.604072762765943E-4 0.0 0.0 6.199383975064342 0.0 26 4.604072762765943E-4 0.0 0.0 6.373023290688658 0.0 27 4.604072762765943E-4 0.0 0.0 6.540085359509022 0.0 28 4.604072762765943E-4 0.0 0.0 6.715895166578641 0.0 29 4.604072762765943E-4 0.0 0.0 6.921960308946437 0.0 30 4.604072762765943E-4 0.0 0.0 7.14867800627864 0.0 31 5.261797443161078E-4 0.0 0.0 7.364345928980205 0.0 32 5.261797443161078E-4 0.0 0.0 7.560019021397757 0.0 33 5.261797443161078E-4 0.0 0.0 7.754442436922559 0.0 34 5.261797443161078E-4 0.0 0.0 7.963730430224291 0.0 35 5.261797443161078E-4 0.0 0.0 8.21787524672897 0.0 36 5.261797443161078E-4 0.0 0.0 8.433608941898575 0.0 37 5.261797443161078E-4 0.0 0.0 8.636648550736552 0.0 38 5.261797443161078E-4 0.0 0.0 8.845607681698088 0.0 39 5.261797443161078E-4 0.0 0.0 9.047858020919591 0.0 40 5.261797443161078E-4 0.0 0.0 9.280363695439272 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGATT 20 6.312099E-4 46.000004 17 ACGTTCC 20 6.312099E-4 46.000004 20 TTAGCCG 20 6.312099E-4 46.000004 11 CTATACG 55 1.8189894E-12 46.000004 1 GACGTAT 35 1.0198528E-7 46.000004 13 TAGCGCG 20 6.312099E-4 46.000004 1 TCCTCGA 20 6.312099E-4 46.000004 26 CGCGACA 35 1.0198528E-7 46.000004 12 GTTCGGA 20 6.312099E-4 46.000004 13 CACGTTC 35 1.0198528E-7 46.000004 46 TTCGCTA 20 6.312099E-4 46.000004 36 CGATCCA 20 6.312099E-4 46.000004 37 ATGTTCG 20 6.312099E-4 46.000004 13 CGATAAG 20 6.312099E-4 46.000004 10 TCGAACG 40 5.6134013E-9 46.000004 1 GCGTAAT 20 6.312099E-4 46.000004 7 TCGCGCG 20 6.312099E-4 46.000004 1 ACGACCC 20 6.312099E-4 46.000004 11 ACGCGAT 30 1.861803E-6 46.0 25 GTCGACG 30 1.861803E-6 46.0 28 >>END_MODULE