FastQCFastQC Report
Thu 26 May 2016
SRR1544744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544744_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1152250
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.36363636363636365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28530.24760251681492734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGG750.046.0000042
TACCCGT253.4171608E-546.016
CGACGAT206.3114613E-446.035
ACGTTCA206.3114613E-446.031
CTATCCG501.6370905E-1146.01
CATTGCG206.3114613E-446.01
CAACCGG650.046.02
CAATTCG301.8614082E-646.01
AAGCGCA253.4171608E-546.024
CGACACA405.6115823E-946.041
TCGATAT405.6115823E-946.017
ATCGTCC253.4171608E-546.015
CCCGTTG301.8614082E-646.045
ATATCGG253.4171608E-546.02
GCCGAAC253.4171608E-546.011
CGTAGGT253.4171608E-546.02
CTACGGC206.3114613E-446.017
CTACGCG301.8614082E-646.019
TATTCGC206.3114613E-446.026
ATCCCGC405.6115823E-946.039
Produced by FastQC (version 0.11.5)