##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544744_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1152250
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.678314601865914	33.0	31.0	34.0	31.0	34.0
2	33.08353308743762	34.0	33.0	34.0	31.0	34.0
3	33.13800130180083	34.0	33.0	34.0	31.0	34.0
4	36.45674376220438	37.0	37.0	37.0	35.0	37.0
5	36.45016012150141	37.0	37.0	37.0	35.0	37.0
6	36.522649598611416	37.0	37.0	37.0	35.0	37.0
7	36.61382078541983	37.0	37.0	37.0	35.0	37.0
8	36.60985289650684	37.0	37.0	37.0	35.0	37.0
9	38.30447125189846	39.0	39.0	39.0	37.0	39.0
10	38.2198403124322	39.0	39.0	39.0	37.0	39.0
11	38.02587546105446	39.0	39.0	39.0	35.0	39.0
12	37.263897591668474	39.0	35.0	39.0	35.0	39.0
13	37.14058754610544	39.0	35.0	39.0	35.0	39.0
14	38.276966804078974	40.0	35.0	41.0	35.0	41.0
15	38.280012150141026	40.0	35.0	41.0	35.0	41.0
16	38.3537287914949	40.0	35.0	41.0	35.0	41.0
17	38.30548665654155	40.0	35.0	41.0	35.0	41.0
18	38.347216315903665	40.0	36.0	41.0	35.0	41.0
19	38.338350184421785	40.0	36.0	41.0	35.0	41.0
20	38.19772792362768	40.0	35.0	41.0	35.0	41.0
21	38.12574701670644	40.0	35.0	41.0	35.0	41.0
22	38.061720980689955	40.0	35.0	41.0	35.0	41.0
23	37.98817444131048	40.0	35.0	41.0	35.0	41.0
24	37.91461314818833	40.0	35.0	41.0	35.0	41.0
25	37.92450683445433	40.0	35.0	41.0	35.0	41.0
26	37.89152874810154	40.0	35.0	41.0	35.0	41.0
27	37.879362985463224	40.0	35.0	41.0	35.0	41.0
28	37.846920373182904	40.0	35.0	41.0	35.0	41.0
29	37.82438880451291	40.0	35.0	41.0	35.0	41.0
30	37.77994445649816	40.0	35.0	41.0	35.0	41.0
31	37.63196702104578	40.0	35.0	41.0	35.0	41.0
32	37.52274766760686	40.0	35.0	41.0	34.0	41.0
33	37.393331308309826	40.0	35.0	41.0	34.0	41.0
34	37.23434410935127	40.0	35.0	41.0	34.0	41.0
35	37.11425905836407	40.0	35.0	41.0	34.0	41.0
36	37.02899023649382	39.0	35.0	41.0	34.0	41.0
37	36.99795964417444	39.0	35.0	41.0	34.0	41.0
38	36.88599349099588	39.0	35.0	41.0	33.0	41.0
39	36.9087871555652	39.0	35.0	41.0	33.0	41.0
40	36.79405250596659	39.0	35.0	41.0	33.0	41.0
41	36.777436320243005	39.0	35.0	41.0	33.0	41.0
42	36.76643002820568	39.0	35.0	41.0	33.0	41.0
43	36.69617357344326	39.0	35.0	41.0	33.0	41.0
44	36.60119244955522	39.0	35.0	41.0	33.0	41.0
45	36.51167715339553	38.0	35.0	41.0	33.0	41.0
46	36.47195573877197	38.0	35.0	41.0	33.0	41.0
47	36.41986287697982	38.0	35.0	41.0	33.0	41.0
48	36.38026296376654	38.0	35.0	41.0	33.0	41.0
49	36.33918507268388	38.0	35.0	41.0	33.0	41.0
50	36.22995443697114	38.0	35.0	40.0	32.0	41.0
51	36.14703406378824	37.0	35.0	40.0	32.0	41.0
52	35.91967888912996	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	0.0
12	5.0
13	9.0
14	16.0
15	16.0
16	59.0
17	82.0
18	158.0
19	325.0
20	619.0
21	1000.0
22	1591.0
23	2627.0
24	4148.0
25	6550.0
26	8869.0
27	9686.0
28	9674.0
29	9854.0
30	10962.0
31	12949.0
32	16084.0
33	22550.0
34	60570.0
35	257018.0
36	44328.0
37	57385.0
38	113937.0
39	499546.0
40	1624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.32410501193318	22.95942720763723	27.714471685832066	14.001996094597526
2	30.96211759600781	25.805510956823607	29.11642438706878	14.115947060099804
3	29.371490561944025	25.363159036667387	31.15625949229768	14.10909090909091
4	25.539162508136254	28.50952484269907	31.084573660229985	14.866738988934694
5	23.08014753742677	32.28075504447819	29.695465393794752	14.943632024300282
6	22.32649164677804	41.916077240182254	25.483619006292034	10.273812106747668
7	87.51555651985247	4.360945975265784	6.138164460837492	1.9853330440442611
8	88.04417444131047	3.3247993057062266	6.475417661097851	2.1556085918854415
9	83.36836623996528	5.698155782165329	7.7664569320893895	3.1670210457799954
10	48.216185723584296	27.047081796485138	13.466435235408984	11.270297244521588
11	44.290735517465826	20.727185940551095	22.21158602733782	12.770492514645259
12	41.8689520503363	20.813451941852897	24.207246691256238	13.110349316554567
13	21.182382295508788	41.22108917335648	23.920503362985464	13.676025168149271
14	14.91898459535691	42.54632241267086	27.581948361900626	14.9527446300716
15	14.056932089390322	24.875417661097853	47.40507702321545	13.662573226296375
16	16.70227815144283	20.560729008461706	45.74580169234107	16.991191147754396
17	16.121848557170754	20.34706009980473	30.60360164894771	32.927489694076804
18	19.535864612714253	23.069125623779563	38.40598828379258	18.989021479713603
19	27.433282707745715	24.73004990236494	28.64300282056845	19.193664569320894
20	30.382903015838576	22.855977435452377	29.58255586895205	17.178563679756998
21	20.12184855717075	29.6249945758299	30.69941418962899	19.553742677370362
22	21.330440442612282	25.150184421783468	26.78524625732263	26.73412887828162
23	16.758776307224995	31.25910175743111	27.41835539162508	24.563766543718813
24	17.138381427641573	22.956302885658495	42.57313950965502	17.33217617704491
25	14.81527446300716	26.48366239965285	38.90032545020612	19.800737687133868
26	15.797960512041659	35.33113473638533	29.102191364721197	19.76871338685181
27	14.961423302234758	37.82642655673682	29.62230418745932	17.589845953569103
28	14.829767845519633	28.97522239097418	39.27880234324149	16.9162074202647
29	15.195139943588632	25.0225211542634	37.817834671295294	21.96450423085268
30	17.678628769798223	34.210978520286396	30.671729225428507	17.438663484486874
31	27.4008244738555	28.34523757865047	27.27359513994359	16.980342807550443
32	28.772488609242785	25.625341722716428	29.52171837708831	16.080451290952485
33	28.729529182035147	27.686960295074854	25.843089607290086	17.740420915599913
34	19.567107832501627	27.27151225862443	28.6936862660013	24.46769364287264
35	19.148275113907573	26.39114775439358	33.76576263831634	20.694814493382513
36	30.160121501410288	25.413148188327185	27.79205901497071	16.63467129529182
37	19.72505966587112	34.53859839444565	30.13217617704491	15.604165762638317
38	19.29060533738338	34.074376220438275	26.214102842265135	20.420915599913215
39	20.150314601865915	33.62456064222174	28.493642872640486	17.73148188327186
40	24.968973747016708	26.172792362768497	27.353612497287916	21.504621392926882
41	16.728661314818833	24.892341071816013	30.23935777825993	28.13963983510523
42	20.509264482534174	23.909134302451726	27.43354306791061	28.148058147103495
43	20.40104144065958	24.56767194619223	29.215448036450425	25.815838576697764
44	17.77036233456281	29.146192232588415	31.702321544803645	21.381123888045128
45	16.97964851377739	38.40182252115426	25.919201562160985	18.699327402907358
46	20.47576480798438	33.99227598177479	27.755348231720546	17.776610978520285
47	19.17101323497505	29.317248860924277	28.046951616402687	23.464786287697983
48	20.70609676719462	24.52401822521154	34.52054675634628	20.249338251247558
49	20.970101974397917	25.370362334562813	34.61939683228466	19.04013885875461
50	19.49307875894988	34.09815578216533	28.05250596658711	18.356259492297678
51	17.43753525710566	34.03584291603385	26.921501410284225	21.605120416576266
52	20.040182252115425	26.723193751356046	35.05237578650466	18.184248210023863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3336.0
1	3354.0
2	3372.0
3	5787.0
4	8202.0
5	5684.0
6	3166.0
7	3289.0
8	3412.0
9	3658.5
10	3905.0
11	4177.5
12	4450.0
13	4361.5
14	4333.5
15	4394.0
16	4073.5
17	3753.0
18	3756.0
19	3759.0
20	3992.5
21	4226.0
22	5065.0
23	5904.0
24	6538.0
25	7172.0
26	10164.0
27	13156.0
28	16020.5
29	18885.0
30	21018.5
31	23152.0
32	26351.5
33	29551.0
34	32408.0
35	35265.0
36	40200.0
37	45135.0
38	49439.0
39	63173.0
40	72603.0
41	95315.0
42	118027.0
43	122647.0
44	127267.0
45	121825.0
46	116383.0
47	108191.5
48	100000.0
49	91372.0
50	82744.0
51	75768.0
52	68792.0
53	60116.5
54	51441.0
55	43032.0
56	34623.0
57	30142.0
58	25661.0
59	23649.5
60	21638.0
61	19772.5
62	17907.0
63	14700.0
64	9523.5
65	7554.0
66	6534.5
67	5515.0
68	4129.0
69	2743.0
70	2578.0
71	2413.0
72	1826.0
73	1239.0
74	1059.0
75	879.0
76	670.0
77	461.0
78	392.0
79	323.0
80	232.0
81	141.0
82	122.0
83	103.0
84	67.5
85	32.0
86	31.5
87	31.0
88	23.5
89	12.0
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1152250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.224689618537546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76947394310316	12.455608869052547
2	9.146870031199947	2.96810254474644
3	3.0710320699517326	1.4947962643062525
4	1.4562533970872953	0.9450903749472909
5	0.8492890436595882	0.68897255649007
6	0.5903766254961261	0.5747206504028536
7	0.4540071374645364	0.5156287422973969
8	0.3540359113860531	0.459529822084382
9	0.29852470173792184	0.435912356624785
>10	4.221214477461311	16.51240878394958
>50	1.1294517974895626	12.973300243975238
>100	1.651399507620507	48.54214339471131
>500	0.005918994650969559	0.6216882303993756
>1k	0.0021523616912616575	0.8120971660124969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4190	0.36363636363636365	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2853	0.24760251681492734	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1026686916901714	0.0
2	0.0	0.0	0.0	0.3205901497070948	0.0
3	0.0	0.0	0.0	0.41657626383163376	0.0
4	0.0	0.0	0.0	0.5256671729225428	0.0
5	0.0	0.0	0.0	0.8222174007376871	0.0
6	0.0	0.0	0.0	1.0402256454762422	0.0
7	0.0	0.0	0.0	1.18472553699284	0.0
8	0.0	0.0	0.0	1.535170318941202	0.0
9	0.0	0.0	0.0	1.6860924278585376	0.0
10	0.0	0.0	0.0	2.035582555868952	0.0
11	0.0	0.0	0.0	2.5062269472770664	0.0
12	0.0	0.0	0.0	2.887047081796485	0.0
13	0.0	0.0	0.0	3.0363202430028204	0.0
14	0.0	0.0	0.0	3.0886526361466697	0.0
15	0.0	0.0	0.0	3.183857669776524	0.0
16	0.0	0.0	0.0	3.392406161857236	0.0
17	0.0	0.0	0.0	3.6462573226296375	0.0
18	0.0	0.0	0.0	3.9856801909307875	0.0
19	0.0	0.0	0.0	4.135560859188544	0.0
20	8.678672163159037E-5	0.0	0.0	4.307485354740725	0.0
21	8.678672163159037E-5	0.0	0.0	4.529659362117596	0.0
22	8.678672163159037E-5	0.0	0.0	4.738121067476676	0.0
23	8.678672163159037E-5	0.0	0.0	5.003948795834237	0.0
24	1.7357344326318074E-4	0.0	0.0	5.186287697982209	0.0
25	3.471468865263615E-4	0.0	0.0	5.347450640052072	0.0
26	3.471468865263615E-4	0.0	0.0	5.493686266001302	0.0
27	3.471468865263615E-4	0.0	0.0	5.645389455413322	0.0
28	4.3393360815795185E-4	0.0	0.0	5.809329572575396	0.0
29	4.3393360815795185E-4	0.0	0.0	5.969277500542417	0.0
30	4.3393360815795185E-4	0.0	0.0	6.1661965719244956	0.0
31	4.3393360815795185E-4	0.0	0.0	6.366326752006943	0.0
32	4.3393360815795185E-4	0.0	0.0	6.545628118897809	0.0
33	4.3393360815795185E-4	0.0	0.0	6.733347797786939	0.0
34	4.3393360815795185E-4	0.0	0.0	6.907702321544804	0.0
35	4.3393360815795185E-4	0.0	0.0	7.13777392059015	0.0
36	4.3393360815795185E-4	0.0	0.0	7.327923627684965	0.0
37	5.207203297895422E-4	0.0	0.0	7.524495552180516	0.0
38	5.207203297895422E-4	0.0	0.0	7.7100455630288565	0.0
39	5.207203297895422E-4	0.0	0.0	7.900195270123671	0.0
40	5.207203297895422E-4	0.0	0.0	8.113690605337384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCGG	75	0.0	46.000004	2
TACCCGT	25	3.4171608E-5	46.0	16
CGACGAT	20	6.3114613E-4	46.0	35
ACGTTCA	20	6.3114613E-4	46.0	31
CTATCCG	50	1.6370905E-11	46.0	1
CATTGCG	20	6.3114613E-4	46.0	1
CAACCGG	65	0.0	46.0	2
CAATTCG	30	1.8614082E-6	46.0	1
AAGCGCA	25	3.4171608E-5	46.0	24
CGACACA	40	5.6115823E-9	46.0	41
TCGATAT	40	5.6115823E-9	46.0	17
ATCGTCC	25	3.4171608E-5	46.0	15
CCCGTTG	30	1.8614082E-6	46.0	45
ATATCGG	25	3.4171608E-5	46.0	2
GCCGAAC	25	3.4171608E-5	46.0	11
CGTAGGT	25	3.4171608E-5	46.0	2
CTACGGC	20	6.3114613E-4	46.0	17
CTACGCG	30	1.8614082E-6	46.0	19
TATTCGC	20	6.3114613E-4	46.0	26
ATCCCGC	40	5.6115823E-9	46.0	39
>>END_MODULE