FastQCFastQC Report
Thu 26 May 2016
SRR1544743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544743_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1232325
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68860.5587811656827542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50400.4089830199014059No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT21850.17730712271519283Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT17990.14598421682591847Illumina Single End Adapter 1 (95% over 22bp)
AATATAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCC17020.1381129166413081No Hit
GAAGAAGGGAGAGTGCTTAATCCTGTGTTTATCCTGTGCAGACAAGTGATTG14700.11928671413791005No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT13320.10808836954537156Illumina Single End Adapter 1 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13280.1077637798470371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG351.0196527E-746.0000041
CGAACTC351.0196527E-746.00000413
CGTATCC551.8189894E-1246.00000425
ATCGTTA351.0196527E-746.00000429
CGTTAGT351.0196527E-746.00000431
TGCGATA351.0196527E-746.00000422
TACGAAC351.0196527E-746.00000445
ACGTCCG700.046.0000041
CAATCGT351.0196527E-746.00000416
CCGTTAA351.0196527E-746.00000430
GCCAACG351.0196527E-746.00000417
CTTAACG253.4173012E-546.01
CGGGTAA206.311633E-446.06
TCTTGCG501.6370905E-1146.01
ACACGTA405.6115823E-946.036
GATCGAT206.311633E-446.08
CACGGAT253.4173012E-546.027
ACGCATT253.4173012E-546.042
CTATCGG253.4173012E-546.01
CGTGCAA253.4173012E-546.026
Produced by FastQC (version 0.11.5)