##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544743_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1232325
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.670268800843935	33.0	31.0	34.0	31.0	34.0
2	33.07099831619094	34.0	33.0	34.0	31.0	34.0
3	33.11139188120017	34.0	33.0	34.0	31.0	34.0
4	36.43979550849005	37.0	37.0	37.0	35.0	37.0
5	36.43796076522021	37.0	37.0	37.0	35.0	37.0
6	36.50914247459071	37.0	37.0	37.0	35.0	37.0
7	36.62064633923681	37.0	37.0	37.0	35.0	37.0
8	36.611035238269125	37.0	37.0	37.0	35.0	37.0
9	38.309099466455685	39.0	39.0	39.0	37.0	39.0
10	38.22411539163776	39.0	39.0	39.0	37.0	39.0
11	38.03790233907451	39.0	39.0	39.0	35.0	39.0
12	37.39956910717546	39.0	35.0	39.0	35.0	39.0
13	37.294722577242204	39.0	35.0	39.0	35.0	39.0
14	38.44557563954314	40.0	35.0	41.0	35.0	41.0
15	38.48788509524679	40.0	35.0	41.0	35.0	41.0
16	38.58334854847544	40.0	35.0	41.0	35.0	41.0
17	38.5506258495121	40.0	35.0	41.0	35.0	41.0
18	38.55382386951494	40.0	36.0	41.0	35.0	41.0
19	38.52923701134035	40.0	36.0	41.0	35.0	41.0
20	38.405489623273084	40.0	35.0	41.0	35.0	41.0
21	38.32263810277321	40.0	35.0	41.0	35.0	41.0
22	38.234889335199725	40.0	35.0	41.0	35.0	41.0
23	38.176899762643785	40.0	35.0	41.0	35.0	41.0
24	38.11850201854219	40.0	35.0	41.0	35.0	41.0
25	38.08741362870996	40.0	35.0	41.0	35.0	41.0
26	38.02424035867162	40.0	35.0	41.0	35.0	41.0
27	38.002542348812206	40.0	35.0	41.0	35.0	41.0
28	37.9830166555089	40.0	35.0	41.0	35.0	41.0
29	37.95812306006938	40.0	35.0	41.0	35.0	41.0
30	37.925116345119996	40.0	35.0	41.0	35.0	41.0
31	37.81653216481042	40.0	35.0	41.0	35.0	41.0
32	37.69394193901771	40.0	35.0	41.0	35.0	41.0
33	37.56338384760514	40.0	35.0	41.0	34.0	41.0
34	37.431221471608545	40.0	35.0	41.0	34.0	41.0
35	37.32523563183413	40.0	35.0	41.0	34.0	41.0
36	37.24491915687826	40.0	35.0	41.0	34.0	41.0
37	37.1821491895401	40.0	35.0	41.0	34.0	41.0
38	37.07445357352971	40.0	35.0	41.0	33.0	41.0
39	37.0741241149859	39.0	35.0	41.0	33.0	41.0
40	36.973704988537925	39.0	35.0	41.0	33.0	41.0
41	36.95173675775465	39.0	35.0	41.0	33.0	41.0
42	36.934366340048285	39.0	35.0	41.0	33.0	41.0
43	36.873560140385045	39.0	35.0	41.0	33.0	41.0
44	36.78736615746658	39.0	35.0	41.0	33.0	41.0
45	36.713095977116424	39.0	35.0	41.0	33.0	41.0
46	36.64190371858073	39.0	35.0	41.0	33.0	41.0
47	36.59192096240846	38.0	35.0	41.0	33.0	41.0
48	36.54626660986347	38.0	35.0	41.0	33.0	41.0
49	36.51046923498266	38.0	35.0	41.0	33.0	41.0
50	36.38461931714442	38.0	35.0	41.0	33.0	41.0
51	36.32801087375489	38.0	35.0	40.0	33.0	41.0
52	36.09579047734972	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	5.0
14	9.0
15	18.0
16	30.0
17	88.0
18	143.0
19	317.0
20	527.0
21	926.0
22	1488.0
23	2420.0
24	3917.0
25	6494.0
26	8811.0
27	10014.0
28	10118.0
29	10503.0
30	11438.0
31	13774.0
32	17603.0
33	23873.0
34	58738.0
35	242938.0
36	60355.0
37	64714.0
38	123024.0
39	558190.0
40	1846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.04787698050433	24.60251962753332	29.428438114945326	13.92116527701702
2	30.064877365954594	25.656137788326944	30.08135029314507	14.197634552573387
3	27.97752216339034	25.523786338831073	32.91031180897897	13.588379688799627
4	25.11431643438216	28.796299677438984	31.42068853589759	14.668695352281258
5	22.25297709613941	34.7249305175177	29.187430263931997	13.83466212241089
6	22.51240541253322	41.799038403018685	25.498468342360987	10.190087842087111
7	88.24100785101334	3.8580731544032623	6.028279877467389	1.8726391171160206
8	88.01306473535796	3.186091331426369	6.1987706165175585	2.602073316698111
9	82.72704035055688	5.880753859574382	8.101271985880349	3.290933803988396
10	43.3632361592924	33.72657375286552	12.661108068082688	10.249082019759397
11	40.17227598239101	20.96354451950581	24.58174588683992	14.282433611263261
12	38.280891810196174	21.463818392063782	25.834094090438803	14.42119570730124
13	22.304992595297506	37.67569431765159	26.487736595459804	13.5315764915911
14	15.272513338607915	40.3545330980058	28.099567889964096	16.27338567342219
15	13.21396547177084	27.416063132696326	44.16302517598848	15.206946219544356
16	16.551640192319397	23.098614407725236	43.15314547704542	17.196599922909947
17	16.073438419248166	22.541618485383317	30.768019799971597	30.616923295396916
18	19.32444769034143	24.650396608037653	36.667924451747716	19.35723124987321
19	25.504635546629338	25.655569756354858	28.83046274318869	20.009331953827115
20	27.99188525754164	25.086645162599154	28.482989471121662	18.438480108737547
21	21.035278842837727	29.128273791410546	31.58679731402025	18.249650051731482
22	20.699166210212404	24.85391434889335	26.980301462682327	27.466617978211914
23	16.58069097032033	30.814923011380923	27.960643499076948	24.643742519221796
24	16.577039336214067	25.378289006552656	40.478364067920396	17.566307589312885
25	16.144604710607997	28.691700647150707	35.08968819102104	20.074006451220257
26	15.571135861075607	35.18219625504636	29.466171667376706	19.780496216501327
27	15.407299210841296	37.65565090377944	28.866816789402144	18.070233095977116
28	13.306716978069907	31.279816606820443	36.45791491692532	18.955551498184324
29	14.185868176008764	25.883269429736472	36.71279897754245	23.21806341671231
30	17.499442111455988	33.91207676546365	30.387113788976123	18.201367334104233
31	25.23603757125758	31.40811068508713	26.858823768080658	16.497027975574625
32	26.15186740510823	28.863165155295885	27.58103584687481	17.403931592721076
33	25.43419958209076	30.758119814172396	24.73576369869961	19.071916905037227
34	18.538129146126224	29.56687562128497	26.212403383847604	25.682591848741197
35	17.529060921429007	29.61564522345972	31.087253768283528	21.768040086827746
36	27.25165845048993	28.403870732152637	26.639319984581988	17.705150832775445
37	17.580508388615016	35.22163390339399	30.383949039417363	16.81390866857363
38	18.250380378552737	34.915140080741686	26.743919014870265	20.09056052583531
39	18.744568194266932	33.957843912928816	28.34211754204451	18.955470350759743
40	23.08149230113809	27.176353640476336	28.723753879861235	21.018400178524335
41	16.87363317306717	26.96504574686061	29.911427586066985	26.24989349400523
42	20.083744142170286	25.53790599070862	28.29919055443978	26.079159312681316
43	19.59182845434443	26.229931227557667	29.435741383157854	24.742498934940052
44	17.03089688191021	29.976345525733876	30.467693181587652	22.525064410768262
45	16.332947883066563	38.10236747611223	25.203497453999553	20.36118718682166
46	19.696914369180206	34.52035786014241	27.226015864321507	18.556711906355872
47	18.91246221573043	30.213377152942613	27.89377802122005	22.98038261010691
48	19.70616517558274	26.998721928062807	33.231817905179234	20.06329499117522
49	20.96849451240541	26.33055403404134	33.46698314162254	19.2339683119307
50	18.44683829346966	34.20015012273548	28.48307061854624	18.869940965248617
51	17.01336903820015	33.39159718418437	27.279897754245024	22.315136023370457
52	19.26001663522204	27.524557239364615	34.34053516726513	18.874890958148217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5797.0
1	4906.0
2	4015.0
3	7552.0
4	11089.0
5	7455.5
6	3822.0
7	4358.5
8	4895.0
9	5407.5
10	5920.0
11	6135.5
12	6351.0
13	6773.0
14	6585.0
15	5975.0
16	5953.5
17	5932.0
18	5228.5
19	4525.0
20	4652.5
21	4780.0
22	5359.5
23	5939.0
24	7487.0
25	9035.0
26	10217.0
27	11399.0
28	14634.0
29	17869.0
30	19724.5
31	21580.0
32	25184.0
33	28788.0
34	33999.5
35	39211.0
36	45699.0
37	52187.0
38	56037.5
39	71257.0
40	82626.0
41	100175.5
42	117725.0
43	127716.5
44	137708.0
45	142644.5
46	147581.0
47	129982.5
48	112384.0
49	96516.5
50	80649.0
51	70956.0
52	61263.0
53	54177.5
54	47092.0
55	42046.0
56	37000.0
57	31362.0
58	25724.0
59	23201.5
60	20679.0
61	17596.5
62	14514.0
63	12282.0
64	8300.5
65	6551.0
66	5941.0
67	5331.0
68	4353.0
69	3375.0
70	2787.0
71	2199.0
72	1839.0
73	1479.0
74	1217.0
75	955.0
76	740.0
77	525.0
78	463.5
79	402.0
80	286.5
81	171.0
82	128.5
83	86.0
84	72.0
85	58.0
86	30.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1232325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.86993243221762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0626926177509	12.494326202095603
2	10.349042932738918	3.4917531002684967
3	3.75690359347268	1.9013612932871908
4	1.9554152719159859	1.3195089405659648
5	1.2214375587975945	1.0302784543544128
6	0.8211729190872704	0.8311878996101495
7	0.5928416706792528	0.7000839249153402
8	0.46709595665588827	0.6303901782517508
9	0.38790406064670985	0.5889523763963557
>10	3.936706167566004	15.689555907502706
>50	1.056671786571098	12.552251122930441
>100	1.3790462299274746	45.97404773512456
>500	0.008712822793504577	0.9262487850209729
>1k	0.00338831997525178	0.8961985731821545
>5k	9.680914215005086E-4	0.9738555064938836
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6886	0.5587811656827542	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5040	0.4089830199014059	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	2185	0.17730712271519283	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	1799	0.14598421682591847	Illumina Single End Adapter 1 (95% over 22bp)
AATATAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCC	1702	0.1381129166413081	No Hit
GAAGAAGGGAGAGTGCTTAATCCTGTGTTTATCCTGTGCAGACAAGTGATTG	1470	0.11928671413791005	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	1332	0.10808836954537156	Illumina Single End Adapter 1 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1328	0.1077637798470371	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1872071085143935	0.0
2	0.0	0.0	0.0	0.5841803095774248	0.0
3	0.0	0.0	0.0	0.8045767147465157	0.0
4	0.0	0.0	0.0	1.0783681252916235	0.0
5	0.0	0.0	0.0	1.7940072626945003	0.0
6	0.0	0.0	0.0	2.3098614407725235	0.0
7	0.0	0.0	0.0	2.674781409125028	0.0
8	0.0	0.0	0.0	3.468322074128172	0.0
9	0.0	0.0	0.0	3.729454486438237	0.0
10	0.0	0.0	0.0	4.205708721319457	0.0
11	0.0	0.0	0.0	4.851561073580427	0.0
12	0.0	0.0	0.0	5.335767756070842	0.0
13	0.0	0.0	0.0	5.513399468484369	0.0
14	0.0	0.0	0.0	5.576207575112085	0.0
15	0.0	0.0	0.0	5.691274623171647	0.0
16	0.0	0.0	0.0	5.940965248615422	0.0
17	0.0	0.0	0.0	6.266934453167792	0.0
18	0.0	0.0	0.0	6.670399448197513	0.0
19	0.0	0.0	0.0	6.8799220984724	0.0
20	8.114742458361227E-5	0.0	0.0	7.102022599557746	0.0
21	8.114742458361227E-5	0.0	0.0	7.370945164627837	0.0
22	8.114742458361227E-5	0.0	0.0	7.675410301665551	0.0
23	8.114742458361227E-5	0.0	0.0	7.991722962692472	0.0
24	3.245896983344491E-4	0.0	0.0	8.241088998437911	0.0
25	3.245896983344491E-4	0.0	0.0	8.444687886718196	0.0
26	3.245896983344491E-4	0.0	0.0	8.637899904651777	0.0
27	3.245896983344491E-4	0.0	0.0	8.832653723652445	0.0
28	3.245896983344491E-4	0.0	0.0	9.046233745156513	0.0
29	3.245896983344491E-4	0.0	0.0	9.273365386566043	0.0
30	3.245896983344491E-4	0.0	0.0	9.53271255553527	0.0
31	3.245896983344491E-4	0.0	0.0	9.788489237822814	0.0
32	3.245896983344491E-4	0.0	0.0	10.007993021321486	0.0
33	3.245896983344491E-4	0.0	0.0	10.236504168948938	0.0
34	3.245896983344491E-4	0.0	0.0	10.459334996855537	0.0
35	3.245896983344491E-4	0.0	0.0	10.756009981133223	0.0
36	4.057371229180614E-4	0.0	0.0	11.009839125230762	0.0
37	4.057371229180614E-4	0.0	0.0	11.234292901629034	0.0
38	4.057371229180614E-4	0.0	0.0	11.472217150508186	0.0
39	4.057371229180614E-4	0.0	0.0	11.724423346114053	0.0
40	4.057371229180614E-4	0.0	0.0	12.000162294849167	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.0196527E-7	46.000004	1
CGAACTC	35	1.0196527E-7	46.000004	13
CGTATCC	55	1.8189894E-12	46.000004	25
ATCGTTA	35	1.0196527E-7	46.000004	29
CGTTAGT	35	1.0196527E-7	46.000004	31
TGCGATA	35	1.0196527E-7	46.000004	22
TACGAAC	35	1.0196527E-7	46.000004	45
ACGTCCG	70	0.0	46.000004	1
CAATCGT	35	1.0196527E-7	46.000004	16
CCGTTAA	35	1.0196527E-7	46.000004	30
GCCAACG	35	1.0196527E-7	46.000004	17
CTTAACG	25	3.4173012E-5	46.0	1
CGGGTAA	20	6.311633E-4	46.0	6
TCTTGCG	50	1.6370905E-11	46.0	1
ACACGTA	40	5.6115823E-9	46.0	36
GATCGAT	20	6.311633E-4	46.0	8
CACGGAT	25	3.4173012E-5	46.0	27
ACGCATT	25	3.4173012E-5	46.0	42
CTATCGG	25	3.4173012E-5	46.0	1
CGTGCAA	25	3.4173012E-5	46.0	26
>>END_MODULE