FastQCFastQC Report
Thu 26 May 2016
SRR1544742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544742_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1089384
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57750.5301161023110308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54550.5007417035682551No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT17880.1641294529752594TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT16540.15182892350172206TruSeq Adapter, Index 14 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14940.1371417241303342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT850.046.0000048
TCACGAC600.046.025
GTGCACG501.6370905E-1146.01
GAGTGTA253.417037E-546.016
AACCGTC206.31131E-446.022
AACGTAA301.8613136E-646.040
CGGGTAT405.6115823E-946.06
AACCGAT206.31131E-446.012
TTGGCGT206.31131E-446.025
CTGTCGG253.417037E-546.02
CGTATTT501.6370905E-1146.017
CGTATTA206.31131E-446.044
TTGGACA301.8613136E-646.030
CGAAACT206.31131E-446.046
CGACGGT600.046.028
CGACGAA206.31131E-446.033
CGGCCTA206.31131E-446.014
ACCATCG301.8613136E-646.042
ACCGCGA301.8613136E-646.044
ATAATCG206.31131E-446.031
Produced by FastQC (version 0.11.5)