##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544742_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1089384
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72039519581708	33.0	31.0	34.0	31.0	34.0
2	33.1230723050825	34.0	33.0	34.0	31.0	34.0
3	33.13926677828938	34.0	33.0	34.0	31.0	34.0
4	36.455897094137605	37.0	37.0	37.0	35.0	37.0
5	36.45248507413364	37.0	37.0	37.0	35.0	37.0
6	36.52270273842832	37.0	37.0	37.0	35.0	37.0
7	36.639514624778776	37.0	37.0	37.0	35.0	37.0
8	36.639123578095514	37.0	37.0	37.0	35.0	37.0
9	38.37358452116058	39.0	39.0	39.0	37.0	39.0
10	38.270308724930786	39.0	39.0	39.0	37.0	39.0
11	38.03839968275649	39.0	39.0	39.0	35.0	39.0
12	37.14283760363655	39.0	35.0	39.0	35.0	39.0
13	36.997723484097435	39.0	35.0	39.0	35.0	39.0
14	38.07585204115353	40.0	35.0	41.0	35.0	41.0
15	38.11997330601514	40.0	35.0	41.0	35.0	41.0
16	38.205629970699036	40.0	35.0	41.0	35.0	41.0
17	38.166261850733996	40.0	35.0	41.0	35.0	41.0
18	38.22568625939063	40.0	36.0	41.0	35.0	41.0
19	38.160604525126125	40.0	36.0	41.0	35.0	41.0
20	37.99053318205518	40.0	35.0	41.0	35.0	41.0
21	37.9178480682661	40.0	35.0	41.0	35.0	41.0
22	37.845140005728005	40.0	35.0	41.0	35.0	41.0
23	37.79262776027553	39.0	35.0	41.0	35.0	41.0
24	37.75088031401232	39.0	35.0	41.0	35.0	41.0
25	37.73647676117879	39.0	35.0	41.0	35.0	41.0
26	37.67035407165885	39.0	35.0	41.0	35.0	41.0
27	37.64144231969627	39.0	35.0	41.0	35.0	41.0
28	37.67107741622789	39.0	35.0	41.0	35.0	41.0
29	37.64047939018748	39.0	35.0	41.0	35.0	41.0
30	37.60420476159003	39.0	35.0	41.0	35.0	41.0
31	37.456504777011595	39.0	35.0	41.0	35.0	41.0
32	37.32025162844323	39.0	35.0	41.0	34.0	41.0
33	37.16347770850315	39.0	35.0	41.0	34.0	41.0
34	37.03011977411087	39.0	35.0	41.0	34.0	41.0
35	36.90052727045743	39.0	35.0	41.0	34.0	41.0
36	36.81108130833572	39.0	35.0	41.0	34.0	41.0
37	36.77701343144383	39.0	35.0	41.0	33.0	41.0
38	36.6548691737716	38.0	35.0	41.0	33.0	41.0
39	36.69282824054695	38.0	35.0	41.0	33.0	41.0
40	36.57269888303849	38.0	35.0	41.0	33.0	41.0
41	36.55789877582193	38.0	35.0	41.0	33.0	41.0
42	36.550293560397435	38.0	35.0	41.0	33.0	41.0
43	36.48654377152592	38.0	35.0	41.0	33.0	41.0
44	36.380836325850204	38.0	35.0	41.0	33.0	41.0
45	36.30760686773443	38.0	35.0	41.0	33.0	41.0
46	36.26738689020584	37.0	35.0	41.0	33.0	41.0
47	36.213131457777976	37.0	35.0	41.0	33.0	41.0
48	36.17552396583758	37.0	35.0	41.0	33.0	41.0
49	36.18517804557438	37.0	35.0	40.0	33.0	41.0
50	36.02292121051897	37.0	35.0	40.0	33.0	41.0
51	35.943800349555346	36.0	35.0	40.0	32.0	41.0
52	35.71559615342248	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	4.0
15	9.0
16	23.0
17	61.0
18	155.0
19	266.0
20	527.0
21	918.0
22	1422.0
23	2414.0
24	3964.0
25	6559.0
26	8953.0
27	9759.0
28	9662.0
29	9488.0
30	10213.0
31	12124.0
32	15626.0
33	21700.0
34	57437.0
35	281336.0
36	42479.0
37	49421.0
38	96250.0
39	446773.0
40	1834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.421151770174703	24.939507097589097	29.147297922495646	14.492043209740551
2	29.712479713305868	26.07813222885594	31.320177274496412	12.88921078334178
3	27.00957605399015	26.82084554206781	32.16010148854765	14.009476915394389
4	24.761975575187446	28.62553516482709	31.982386376153865	14.630102883831597
5	21.93166046132493	34.067601506906655	29.831813208198394	14.168924823570016
6	21.745867389276878	41.858793593443636	25.893532491756808	10.50180652552268
7	88.09418900956871	4.393033126978183	5.985859898805197	1.526917964647911
8	88.95375735277918	3.1798704589015445	5.840089445044172	2.0262827432750985
9	84.06705073693023	5.197891652530237	7.918970721068053	2.8160868894714812
10	50.96201155882591	28.338767597100745	11.741038972483532	8.958181871589815
11	48.02686655945011	18.42747828130393	21.325629897263042	12.220025261982919
12	44.7138015612493	20.49387543786213	22.14590998215505	12.646413018733524
13	20.34782959911289	43.332562255366334	23.656763822490507	12.662844323030262
14	13.988088681309804	46.14571170496354	26.458806077563104	13.407393536163555
15	12.485129210636469	23.75314856836524	51.36967313637799	12.392049084620298
16	14.075661107561704	19.84736328053285	49.24902513714172	16.82795047476372
17	14.068684687860294	19.36562314115133	29.50309532726752	37.062596843720854
18	18.182569231786037	22.378977477179767	39.46597343085634	19.97247986017786
19	27.626163042600222	23.113062060760946	28.02693999544697	21.233834901191866
20	29.566158489568416	22.418173940502157	29.462889118988343	18.552778450941084
21	19.09427713276494	30.771426787982936	32.039299273717994	18.094996805534137
22	20.662319255652736	23.250662759871634	26.99084987479163	29.096168109684008
23	15.113128153158115	32.1080537257753	26.848659425877376	25.93015869518921
24	15.766524935192733	22.46177656363596	44.66973996313513	17.101958538036175
25	14.13624580496868	25.323852746139103	40.98857703068891	19.551324418203315
26	14.081444192314189	36.943722323808686	28.377688675434925	20.597144808442202
27	13.68433903931029	40.61928576149457	29.151336902322782	16.54503829687236
28	12.869474859186475	31.90638011940693	39.08915497198417	16.134990049422427
29	13.131365982977536	25.159080728191345	38.57804043385987	23.131512854971252
30	15.900270244468434	36.1233504439206	30.308229237807787	17.668150073803176
31	28.989410529253227	28.584502801583277	27.228598914615965	15.197487754547524
32	30.013567300419318	24.600049202117894	30.15043363956144	15.235949857901346
33	28.244953111116004	27.90274136576267	26.38849111057258	17.463814412548743
34	18.86763528746521	28.77277433852526	26.96377035095063	25.395820023058903
35	18.526616877060796	25.737756383424028	33.44247758366196	22.293149155853218
36	32.51911171818202	24.78969766400094	26.696371527395296	15.994819090421744
37	18.51495891255976	34.777085031540764	31.29566801054541	15.412288045354073
38	17.561025313388118	34.92267189530964	26.07427683902095	21.44202595228129
39	20.108061069374987	32.77760642711844	29.21917340441938	17.89515909908719
40	25.911157131002476	24.57407121822975	27.087418210658498	22.427353440109275
41	15.124051757690586	24.87892240018212	30.70138720598063	29.295638636146666
42	19.60236243601889	22.842634002335267	29.640512436386068	27.914491125259776
43	20.625509462228194	23.851919984137826	29.354938203608643	26.167632350025332
44	16.596902469652576	29.181996431010553	31.999735630411312	22.22136546892556
45	15.177660035396151	41.6945723454723	24.04744332576943	19.080324293362118
46	21.69914373627665	34.43496508118349	26.57208110271493	17.29381007982493
47	18.54020253647933	29.275994506987434	27.65406872140586	24.529734235127375
48	20.55647962518267	25.72334456904085	35.183094299163564	18.537081506612914
49	22.805732413914654	24.498248551474962	34.496743113539395	18.19927592107099
50	21.34591659139477	34.34317008511232	27.031606853047226	17.279306470445682
51	17.49080214139367	33.75476416029609	26.095022508133038	22.6594111901772
52	19.54076799365513	26.417039354350713	35.66786367341543	18.37432897857872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6180.0
1	5190.5
2	4201.0
3	6999.5
4	9798.0
5	6556.0
6	3314.0
7	3538.0
8	3762.0
9	4255.0
10	4748.0
11	4689.5
12	4631.0
13	4663.0
14	4399.5
15	4104.0
16	4387.0
17	4670.0
18	4440.5
19	4211.0
20	4450.0
21	4689.0
22	5028.0
23	5367.0
24	5947.0
25	6527.0
26	7660.0
27	8793.0
28	12398.0
29	16003.0
30	17208.5
31	18414.0
32	20193.0
33	21972.0
34	27663.5
35	33355.0
36	33319.0
37	33283.0
38	37807.0
39	59385.0
40	76439.0
41	98119.5
42	119800.0
43	125195.5
44	130591.0
45	123206.5
46	115822.0
47	110262.5
48	104703.0
49	96127.5
50	87552.0
51	73988.5
52	60425.0
53	50985.5
54	41546.0
55	35474.5
56	29403.0
57	25268.5
58	21134.0
59	19176.0
60	17218.0
61	15239.0
62	13260.0
63	11035.5
64	7553.0
65	6295.0
66	5246.0
67	4197.0
68	3374.0
69	2551.0
70	2234.5
71	1918.0
72	1492.5
73	1067.0
74	869.5
75	672.0
76	573.5
77	475.0
78	371.0
79	267.0
80	162.5
81	58.0
82	89.5
83	121.0
84	63.5
85	6.0
86	3.0
87	0.0
88	1.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1089384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.970232663650169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10207113501592	11.993975495482099
2	10.416423014054342	3.32705398114896
3	3.7444484494385137	1.793991388037315
4	1.8018244134936925	1.151022204101571
5	1.049132093447003	0.8377441813625504
6	0.6897715118478819	0.6609486917381042
7	0.5007498797878915	0.5597964460555236
8	0.38511545872954595	0.492030678262337
9	0.3158037625579562	0.4539113607696025
>10	3.431404363482646	13.052452485031122
>50	1.0536224439183073	11.833351792292655
>100	1.4905607695366503	50.71146391428834
>500	0.016182900948827534	1.6401084841793732
>1k	0.0017338822445172356	0.45560107366252384
>5k	0.0011559214963448238	1.0365478235879542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5775	0.5301161023110308	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5455	0.5007417035682551	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	1788	0.1641294529752594	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1654	0.15182892350172206	TruSeq Adapter, Index 14 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1494	0.1371417241303342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17193202764130922	0.0
2	0.0	0.0	0.0	0.5155206979357141	0.0
3	0.0	0.0	0.0	0.7166435343276567	0.0
4	0.0	0.0	0.0	0.9394299897923964	0.0
5	0.0	0.0	0.0	1.5574856983396121	0.0
6	0.0	0.0	0.0	1.9894729498505577	0.0
7	0.0	0.0	0.0	2.277892827506187	0.0
8	0.0	0.0	0.0	2.849133088057104	0.0
9	0.0	0.0	0.0	3.066411843757573	0.0
10	0.0	0.0	0.0	3.467464181592533	0.0
11	0.0	0.0	0.0	4.016398258098155	0.0
12	0.0	0.0	0.0	4.457014239239791	0.0
13	0.0	0.0	0.0	4.633444221688587	0.0
14	0.0	0.0	0.0	4.685491984460943	0.0
15	0.0	0.0	0.0	4.791974179903505	0.0
16	0.0	0.0	0.0	5.03394578954712	0.0
17	0.0	0.0	0.0	5.313920527564202	0.0
18	0.0	0.0	0.0	5.711576450544528	0.0
19	0.0	0.0	0.0	5.90407055730578	0.0
20	0.0	0.0	0.0	6.114005713320555	0.0
21	0.0	0.0	0.0	6.3797522269466045	0.0
22	0.0	0.0	0.0	6.645039765592298	0.0
23	0.0	0.0	0.0	6.9724725165781765	0.0
24	9.179499607117417E-5	0.0	0.0	7.189108707306147	0.0
25	9.179499607117417E-5	0.0	0.0	7.3601227849867445	0.0
26	9.179499607117417E-5	0.0	0.0	7.548761501913008	0.0
27	9.179499607117417E-5	0.0	0.0	7.723080199452167	0.0
28	9.179499607117417E-5	0.0	0.0	7.906394806606302	0.0
29	9.179499607117417E-5	0.0	0.0	8.10632430804932	0.0
30	9.179499607117417E-5	0.0	0.0	8.358393367260764	0.0
31	9.179499607117417E-5	0.0	0.0	8.595591637108678	0.0
32	9.179499607117417E-5	0.0	0.0	8.802681148245247	0.0
33	9.179499607117417E-5	0.0	0.0	9.017481439051794	0.0
34	9.179499607117417E-5	0.0	0.0	9.224570950188363	0.0
35	9.179499607117417E-5	0.0	0.0	9.514734932769345	0.0
36	9.179499607117417E-5	0.0	0.0	9.730636763528747	0.0
37	9.179499607117417E-5	0.0	0.0	9.957186813832404	0.0
38	9.179499607117417E-5	0.0	0.0	10.176301469454296	0.0
39	9.179499607117417E-5	0.0	0.0	10.416620769168631	0.0
40	9.179499607117417E-5	0.0	0.0	10.67447291313256	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGCGT	85	0.0	46.000004	8
TCACGAC	60	0.0	46.0	25
GTGCACG	50	1.6370905E-11	46.0	1
GAGTGTA	25	3.417037E-5	46.0	16
AACCGTC	20	6.31131E-4	46.0	22
AACGTAA	30	1.8613136E-6	46.0	40
CGGGTAT	40	5.6115823E-9	46.0	6
AACCGAT	20	6.31131E-4	46.0	12
TTGGCGT	20	6.31131E-4	46.0	25
CTGTCGG	25	3.417037E-5	46.0	2
CGTATTT	50	1.6370905E-11	46.0	17
CGTATTA	20	6.31131E-4	46.0	44
TTGGACA	30	1.8613136E-6	46.0	30
CGAAACT	20	6.31131E-4	46.0	46
CGACGGT	60	0.0	46.0	28
CGACGAA	20	6.31131E-4	46.0	33
CGGCCTA	20	6.31131E-4	46.0	14
ACCATCG	30	1.8613136E-6	46.0	42
ACCGCGA	30	1.8613136E-6	46.0	44
ATAATCG	20	6.31131E-4	46.0	31
>>END_MODULE