FastQCFastQC Report
Thu 26 May 2016
SRR1544741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544741_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1426489
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.42776355092818796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53830.37736007778538777No Hit
ATGGCAGGGAGTGTTTGGAATTACGTTTTCTGTTCATACAAACCGGGAGAGA28280.1982489875491504No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT24540.17203076925233915Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT18180.1274457777101681No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16220.11370574887012799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA1450.046.00000424
GTCGATC206.3119695E-446.016
CTCGTCT253.417573E-546.019
GATCGCC301.8617211E-646.09
TTTACGA453.110472E-1046.015
CAACGAC206.3119695E-446.012
CGACGGA453.110472E-1046.019
ACGCATG453.110472E-1046.01
GACACGT301.8617211E-646.018
GACACGA600.046.025
CGGCCGT206.3119695E-446.015
CGGTTTA253.417573E-546.010
CGGTTGA253.417573E-546.042
GACGTAA253.417573E-546.09
CGTTGGC405.6134013E-946.040
TGTGGCG206.3119695E-446.033
CCGGATA501.6370905E-1146.020
CGCGCAT206.3119695E-446.042
ATGCTCG301.8617211E-646.034
CAATTCG206.3119695E-446.027
Produced by FastQC (version 0.11.5)