##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544741_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1426489
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66221611242708	33.0	31.0	34.0	31.0	34.0
2	33.06595073638843	34.0	33.0	34.0	31.0	34.0
3	33.10788937033514	34.0	33.0	34.0	31.0	34.0
4	36.42844143908575	37.0	37.0	37.0	35.0	37.0
5	36.424987504285	37.0	37.0	37.0	35.0	37.0
6	36.49884857156277	37.0	37.0	37.0	35.0	37.0
7	36.625952250595695	37.0	37.0	37.0	35.0	37.0
8	36.624573340558534	37.0	37.0	37.0	35.0	37.0
9	38.354335715172006	39.0	39.0	39.0	37.0	39.0
10	38.21616640576969	39.0	39.0	39.0	37.0	39.0
11	38.00675644887553	39.0	39.0	39.0	35.0	39.0
12	37.275118139712255	39.0	35.0	39.0	35.0	39.0
13	37.148369878772286	39.0	35.0	39.0	35.0	39.0
14	38.284240537431415	40.0	35.0	41.0	35.0	41.0
15	38.32617356320308	40.0	35.0	41.0	35.0	41.0
16	38.40904556572115	40.0	35.0	41.0	35.0	41.0
17	38.3482347217539	40.0	35.0	41.0	35.0	41.0
18	38.38543935494771	40.0	36.0	41.0	35.0	41.0
19	38.361240780686	40.0	36.0	41.0	35.0	41.0
20	38.24420097175653	40.0	35.0	41.0	35.0	41.0
21	38.16864903970518	40.0	35.0	41.0	35.0	41.0
22	38.11716318878028	40.0	35.0	41.0	35.0	41.0
23	38.04579355326259	40.0	35.0	41.0	35.0	41.0
24	37.98655089523999	40.0	35.0	41.0	35.0	41.0
25	37.97483051043506	40.0	35.0	41.0	35.0	41.0
26	37.91777433965491	40.0	35.0	41.0	35.0	41.0
27	37.892074176527125	40.0	35.0	41.0	35.0	41.0
28	37.886234664270106	40.0	35.0	41.0	35.0	41.0
29	37.869476035216536	40.0	35.0	41.0	35.0	41.0
30	37.83468712341981	40.0	35.0	41.0	35.0	41.0
31	37.71953937254336	40.0	35.0	41.0	35.0	41.0
32	37.58798140048749	40.0	35.0	41.0	35.0	41.0
33	37.46162711384385	40.0	35.0	41.0	34.0	41.0
34	37.34638963216681	40.0	35.0	41.0	34.0	41.0
35	37.236633440566315	40.0	35.0	41.0	34.0	41.0
36	37.16814991212691	40.0	35.0	41.0	34.0	41.0
37	37.13577251559598	40.0	35.0	41.0	34.0	41.0
38	37.03694876020776	40.0	35.0	41.0	33.0	41.0
39	37.050980414149706	39.0	35.0	41.0	34.0	41.0
40	36.93740926148046	39.0	35.0	41.0	33.0	41.0
41	36.94499291617391	39.0	35.0	41.0	34.0	41.0
42	36.932160710667944	39.0	35.0	41.0	33.0	41.0
43	36.874969943686914	39.0	35.0	41.0	33.0	41.0
44	36.795021903428626	39.0	35.0	41.0	33.0	41.0
45	36.71346922408795	39.0	35.0	41.0	33.0	41.0
46	36.66240328526894	39.0	35.0	41.0	33.0	41.0
47	36.597007758209145	38.0	35.0	41.0	33.0	41.0
48	36.540503291648236	38.0	35.0	41.0	33.0	41.0
49	36.529204220992945	38.0	35.0	41.0	33.0	41.0
50	36.39195535331853	38.0	35.0	41.0	33.0	41.0
51	36.33791427764252	38.0	35.0	41.0	33.0	41.0
52	36.11249578510595	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	1.0
14	3.0
15	12.0
16	30.0
17	90.0
18	185.0
19	422.0
20	637.0
21	1094.0
22	1741.0
23	3025.0
24	4889.0
25	7733.0
26	10641.0
27	11743.0
28	11677.0
29	11885.0
30	13254.0
31	15817.0
32	20131.0
33	27773.0
34	70451.0
35	310802.0
36	57666.0
37	68228.0
38	133340.0
39	640674.0
40	2538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.906800543151753	24.524128822584682	30.03282885462138	13.536241779642186
2	29.006392618520017	25.961504084503982	31.549279384558872	13.48282391241713
3	28.091769372213875	26.15316346638495	31.92082098074363	13.834246180657544
4	25.732550338628617	27.750301614663698	32.48675594413977	14.030392102567912
5	21.60500361376779	34.37839338403591	28.784098580500796	15.232504421695506
6	21.956005268880446	42.889079411057494	25.617863159127058	9.537052160934994
7	89.252913972698	3.8553399290145243	5.2442745790538865	1.6474715192335867
8	89.55806879688521	3.0945208830912825	5.368004940802208	1.979405379221291
9	85.23984412077485	5.421913523342977	6.7508407004891025	2.5874016553930663
10	46.87894543876609	32.12089262517973	11.640678617220322	9.359483318833863
11	42.82339366093955	20.193425957017546	23.332251422899162	13.650928959143743
12	40.56189707736968	20.530477276726284	24.96682413954822	13.940801506355815
13	21.705179640361756	38.838715195139955	25.224800191238767	14.23130497325952
14	14.213779426269674	42.209018085663466	28.3686029124655	15.208599575601353
15	14.016441767163995	24.620869841968638	48.15165066116879	13.211037729698582
16	16.635950224642464	20.95852123640631	45.48538404432141	16.92014449462982
17	16.257328307473802	20.546039962453268	29.963357586353627	33.2332741437193
18	19.23246516447025	24.01189213516543	38.03050707015617	18.725135630208154
19	26.479278844772026	25.422067748156486	28.478032427870104	19.62062097920138
20	28.757670055640105	25.082562851869174	28.407439524595002	17.75232756789572
21	20.394969747400786	30.031216504298314	31.076019513645043	18.497794234655856
22	21.04537784728799	23.87463205114095	27.179108987170597	27.900881114400462
23	16.726241842734154	31.658779002151437	27.04367156003306	24.57130759508135
24	16.37727315107232	23.314024854029718	42.66846782554931	17.64023416934866
25	15.22409215914038	26.809670456624623	37.10719115254306	20.859046231691938
26	15.573271157366092	34.966761047579055	29.507623262429643	19.952344532625208
27	15.128893387891528	37.896261380213936	28.647960131483664	18.32688510041087
28	13.147384943031456	30.64832606490481	39.0447455255526	17.159543466511135
29	14.747327178828579	25.1567309667302	37.176241807683056	22.91970004675816
30	17.17040930564484	32.452335769851715	31.10931805292575	19.2679368715777
31	27.10893669702325	28.608773008414367	26.48516742856061	17.79712286600177
32	28.406598298339492	25.82515532892297	28.420899144683204	17.347347228054335
33	26.701642984979202	28.990689728417113	24.900857980678435	19.406809305925247
34	18.566914991983815	27.675572682298988	28.442700925138574	25.314811400578623
35	17.70318593413619	27.809678167865297	31.770101276630946	22.717034621367567
36	28.538951229206816	26.789761435244152	26.880754075215442	17.79053326033359
37	18.421733360719923	35.31145350577537	29.634648427012056	16.632164706492656
38	17.521691369509334	35.510964332707786	25.576853379170817	21.39049091861206
39	18.851599977286888	33.76352709344411	28.300603790144898	19.084269139124103
40	23.841964431551872	25.82880064269686	28.112309313285976	22.216925612465293
41	15.60670990102272	25.494623512694453	29.265350100841996	29.633316485440826
42	18.659800391030004	25.029215086832075	27.78626403708686	28.52472048505106
43	18.63281104866564	24.7352065105304	29.88862865398892	26.74335378681504
44	16.320630583201133	29.056725989474856	32.36870385961616	22.253939567707846
45	16.112146676209914	38.08133115642672	25.560028854060562	20.246493313302803
46	19.54364877682197	34.51277927835405	26.87297273235195	19.07059921247202
47	19.083638219432466	28.211433807060555	27.628814522930075	25.076113450576905
48	19.20940154463161	26.445489590175598	33.92251885573601	20.422590009456783
49	20.852596830399673	25.659083245647178	34.16654457202264	19.32177535193051
50	18.846342313189936	34.33927636315457	27.577009006028085	19.237372317627404
51	17.146644663926605	33.77558466977313	26.440161823890683	22.63760884240958
52	18.354855873406663	27.10185637603935	35.42494894808162	19.118338802472366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6209.0
1	5370.0
2	4531.0
3	7915.0
4	11299.0
5	7519.0
6	3739.0
7	4015.0
8	4291.0
9	4560.5
10	4830.0
11	4855.0
12	4880.0
13	4975.5
14	5013.0
15	4955.0
16	4856.0
17	4757.0
18	4454.5
19	4152.0
20	4750.5
21	5349.0
22	6295.5
23	7242.0
24	8883.0
25	10524.0
26	11093.5
27	11663.0
28	14955.5
29	18248.0
30	21205.5
31	24163.0
32	28953.5
33	33744.0
34	39075.0
35	44406.0
36	50248.5
37	56091.0
38	63908.5
39	89550.5
40	107375.0
41	126600.5
42	145826.0
43	149940.5
44	154055.0
45	151310.0
46	148565.0
47	130596.5
48	112628.0
49	112321.5
50	112015.0
51	103889.0
52	95763.0
53	79482.5
54	63202.0
55	54283.5
56	45365.0
57	39361.5
58	33358.0
59	28059.5
60	22761.0
61	20434.5
62	18108.0
63	14895.0
64	8543.5
65	5405.0
66	4301.5
67	3198.0
68	2490.5
69	1783.0
70	1527.5
71	1272.0
72	1090.0
73	908.0
74	773.5
75	639.0
76	470.5
77	302.0
78	283.0
79	264.0
80	190.5
81	117.0
82	68.5
83	20.0
84	12.0
85	4.0
86	2.0
87	0.0
88	1.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1426489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.745355167681263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07860901305018	11.663940092382695
2	10.792812922251807	3.398733454383892
3	3.932868113469654	1.8577321582268485
4	1.9679969450370431	1.2394724347407977
5	1.1148964044151288	0.8777219931343503
6	0.775437311956851	0.7325721532219602
7	0.5307281250562836	0.584955197854212
8	0.429206299442953	0.5406404499948362
9	0.33040144443810904	0.4682059281533641
>10	3.5206710978595424	13.011716562553616
>50	1.0391915915403747	11.559338541369677
>100	1.459433152381256	49.97865934805361
>500	0.023272163116781756	2.304130430021977
>1k	0.003580332787197193	0.9728675979229131
>5k	8.950831967992983E-4	0.8093136579852713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6102	0.42776355092818796	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5383	0.37736007778538777	No Hit
ATGGCAGGGAGTGTTTGGAATTACGTTTTCTGTTCATACAAACCGGGAGAGA	2828	0.1982489875491504	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	2454	0.17203076925233915	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1818	0.1274457777101681	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1622	0.11370574887012799	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17869047710848104	0.0
2	0.0	0.0	0.0	0.5132181180506825	0.0
3	0.0	0.0	0.0	0.6912075732795696	0.0
4	0.0	0.0	0.0	0.9304663407849623	0.0
5	0.0	0.0	0.0	1.5466645729479862	0.0
6	0.0	0.0	0.0	1.9748487370039307	0.0
7	0.0	0.0	0.0	2.2816860137021737	0.0
8	0.0	0.0	0.0	2.988316068332809	0.0
9	0.0	0.0	0.0	3.2162182813887803	0.0
10	0.0	0.0	0.0	3.644612752008603	0.0
11	0.0	0.0	0.0	4.20318698566901	0.0
12	0.0	0.0	0.0	4.668385104967511	0.0
13	0.0	0.0	0.0	4.833055144484114	0.0
14	0.0	0.0	0.0	4.886613216085087	0.0
15	0.0	0.0	0.0	4.986228425175378	0.0
16	0.0	0.0	0.0	5.218056360757076	0.0
17	0.0	0.0	0.0	5.508139214533025	0.0
18	0.0	0.0	0.0	5.902744430556422	0.0
19	7.01021879593884E-5	0.0	0.0	6.094824425565147	0.0
20	7.01021879593884E-5	0.0	0.0	6.310809266668022	0.0
21	2.103065638781652E-4	0.0	0.0	6.579791361868195	0.0
22	0.001051532819390826	0.0	0.0	6.8837544488601035	0.0
23	0.001051532819390826	0.0	0.0	7.212323403825756	0.0
24	0.0022432700147004287	0.0	0.0	7.450881149451556	0.0
25	0.0022432700147004287	0.0	0.0	7.652985757338472	0.0
26	0.0022432700147004287	0.0	0.0	7.860067620570506	0.0
27	0.0022432700147004287	0.0	0.0	8.052778535270864	0.0
28	0.0022432700147004287	0.0	0.0	8.259159376623304	0.0
29	0.0022432700147004287	0.0	0.0	8.484117297784982	0.0
30	0.0022432700147004287	0.0	0.0	8.76733013714091	0.0
31	0.0022432700147004287	0.0	0.0	9.039817341739052	0.0
32	0.002383474390619206	0.0	0.0	9.272065890448507	0.0
33	0.002383474390619206	0.0	0.0	9.511745271081656	0.0
34	0.002383474390619206	0.0	0.0	9.752826695473992	0.0
35	0.002383474390619206	0.0	0.0	10.053705286195687	0.0
36	0.002453576578578594	0.0	0.0	10.309928783187251	0.0
37	0.002453576578578594	0.0	0.0	10.565521360487182	0.0
38	0.002453576578578594	0.0	0.0	10.829946813469995	0.0
39	0.002593780954497371	0.0	0.0	11.102994835571813	0.0
40	0.002593780954497371	0.0	0.0	11.395040550610625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	145	0.0	46.000004	24
GTCGATC	20	6.3119695E-4	46.0	16
CTCGTCT	25	3.417573E-5	46.0	19
GATCGCC	30	1.8617211E-6	46.0	9
TTTACGA	45	3.110472E-10	46.0	15
CAACGAC	20	6.3119695E-4	46.0	12
CGACGGA	45	3.110472E-10	46.0	19
ACGCATG	45	3.110472E-10	46.0	1
GACACGT	30	1.8617211E-6	46.0	18
GACACGA	60	0.0	46.0	25
CGGCCGT	20	6.3119695E-4	46.0	15
CGGTTTA	25	3.417573E-5	46.0	10
CGGTTGA	25	3.417573E-5	46.0	42
GACGTAA	25	3.417573E-5	46.0	9
CGTTGGC	40	5.6134013E-9	46.0	40
TGTGGCG	20	6.3119695E-4	46.0	33
CCGGATA	50	1.6370905E-11	46.0	20
CGCGCAT	20	6.3119695E-4	46.0	42
ATGCTCG	30	1.8617211E-6	46.0	34
CAATTCG	20	6.3119695E-4	46.0	27
>>END_MODULE