FastQCFastQC Report
Thu 26 May 2016
SRR1544739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544739_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1154458
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79500.6886348398988963No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.3753276429285431No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT26920.23318301748526146Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24590.21300038632847623No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT22390.19394382472121116Illumina Single End Adapter 2 (95% over 21bp)
GTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT14080.12196199428649636No Hit
AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT13750.11910351004540659No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT13120.1136464037669625Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC453.092282E-1046.00000425
ATTACTA453.092282E-1046.00000428
CACGCAC453.092282E-1046.00000445
ATTCGCG900.046.0000041
CAATTAC453.092282E-1046.00000426
TAAGTAC453.092282E-1046.00000424
CACGACG453.092282E-1046.00000426
GTTACCA453.092282E-1046.00000412
CGTAATA453.092282E-1046.00000433
CGGTCTA453.092282E-1046.00000431
GAACCAC453.092282E-1046.00000438
GTAAGTA453.092282E-1046.00000423
ATACGAG453.092282E-1046.0000041
TGCAGAT453.092282E-1046.00000440
TCACGGT206.3114666E-446.033
CCTTCGC501.6370905E-1146.027
GTTTGCG600.046.01
CTTCGCA206.3114666E-446.014
AAGTATG800.046.01
AGCGTAT301.86141E-646.033