##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544739_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1154458
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70099042147917	33.0	31.0	34.0	31.0	34.0
2	33.08925054008028	34.0	33.0	34.0	31.0	34.0
3	33.111098021755666	34.0	33.0	34.0	31.0	34.0
4	36.437583697284786	37.0	37.0	37.0	35.0	37.0
5	36.43422541140518	37.0	37.0	37.0	35.0	37.0
6	36.50631118672139	37.0	37.0	37.0	35.0	37.0
7	36.62904323933829	37.0	37.0	37.0	35.0	37.0
8	36.63165225586379	37.0	37.0	37.0	35.0	37.0
9	38.396580040157374	39.0	39.0	39.0	37.0	39.0
10	38.26782611407258	39.0	39.0	39.0	37.0	39.0
11	37.972626981665854	39.0	38.0	39.0	35.0	39.0
12	36.7198798050687	38.0	35.0	39.0	35.0	39.0
13	36.5121554876834	37.0	35.0	39.0	35.0	39.0
14	37.36587125733461	38.0	35.0	41.0	35.0	41.0
15	37.47779564089815	38.0	35.0	41.0	35.0	41.0
16	37.603150569358085	38.0	35.0	41.0	35.0	41.0
17	37.5742157791795	38.0	35.0	41.0	35.0	41.0
18	37.66927770434265	38.0	35.0	41.0	35.0	41.0
19	37.627778576613444	37.0	36.0	41.0	35.0	41.0
20	37.39554059134243	36.0	35.0	41.0	35.0	41.0
21	37.28128264518934	36.0	35.0	41.0	35.0	41.0
22	37.2261061034702	36.0	35.0	41.0	35.0	41.0
23	37.175015461801124	35.0	35.0	41.0	35.0	41.0
24	37.1333344305293	35.0	35.0	41.0	35.0	41.0
25	37.118457319365454	35.0	35.0	41.0	35.0	41.0
26	37.05262556108581	35.0	35.0	41.0	35.0	41.0
27	37.042330686781156	35.0	35.0	41.0	35.0	41.0
28	37.11317345455616	36.0	35.0	41.0	35.0	41.0
29	37.13500101346259	36.0	35.0	41.0	35.0	41.0
30	37.10837466586052	36.0	35.0	41.0	35.0	41.0
31	36.891384528497355	36.0	35.0	41.0	34.0	41.0
32	36.73413931039501	35.0	35.0	41.0	34.0	41.0
33	36.60109765794858	35.0	35.0	41.0	34.0	41.0
34	36.48697050910471	35.0	35.0	41.0	34.0	41.0
35	36.36450178352092	35.0	35.0	41.0	34.0	41.0
36	36.26950135907933	35.0	35.0	41.0	33.0	41.0
37	36.22500602014105	35.0	35.0	41.0	33.0	41.0
38	36.13992973326011	35.0	35.0	41.0	33.0	41.0
39	36.17329863884178	35.0	35.0	41.0	33.0	41.0
40	36.03754662361039	35.0	35.0	41.0	32.0	41.0
41	36.09578260967484	36.0	35.0	41.0	33.0	41.0
42	36.08900193857204	35.0	35.0	41.0	33.0	41.0
43	36.01509972645172	35.0	35.0	40.0	33.0	41.0
44	35.924097715118265	35.0	35.0	40.0	33.0	41.0
45	35.86465510222113	35.0	35.0	40.0	33.0	41.0
46	35.85668512843257	35.0	35.0	40.0	33.0	41.0
47	35.77502516332339	35.0	35.0	40.0	33.0	41.0
48	35.77229314535479	35.0	35.0	40.0	33.0	41.0
49	35.8127536904764	36.0	35.0	40.0	33.0	41.0
50	35.66094998692027	35.0	35.0	40.0	33.0	41.0
51	35.586567895930386	35.0	35.0	40.0	32.0	41.0
52	35.35319777765844	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	4.0
14	6.0
15	13.0
16	35.0
17	76.0
18	187.0
19	369.0
20	628.0
21	1132.0
22	1790.0
23	2893.0
24	4718.0
25	7226.0
26	9325.0
27	10304.0
28	9734.0
29	9619.0
30	10761.0
31	13148.0
32	17343.0
33	25094.0
34	71244.0
35	402459.0
36	43286.0
37	43678.0
38	82101.0
39	385344.0
40	1937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.28328791519483	25.139242830834903	29.852796723657338	12.724672530312924
2	29.873585699956166	26.07708552411608	30.97046406192343	13.07886471400432
3	26.7788867156709	26.577926611448838	33.22589474887783	13.417291924002434
4	23.943097107040707	29.474004251345654	32.00393604617924	14.578962595434394
5	22.04662274417952	32.811501154654394	30.943005289062054	14.198870812104037
6	20.66805375336305	41.523294914150185	26.815787148601334	10.992864183885425
7	87.50010827591822	5.08351105020711	5.807833632752339	1.6085470411223275
8	88.73930450479793	3.2189997384053814	5.800470870313169	2.2412248864835274
9	85.2858224378886	4.52021641324327	7.472597530616099	2.7213636182520284
10	60.6464678663061	19.90102714867063	10.7200954906978	8.732409494325475
11	56.32028189851861	16.505840836132627	17.142503235284437	10.031374030064324
12	50.886476597676136	19.362765903999975	19.56996270111169	10.180794797212197
13	18.66590209431612	52.23100363980326	20.0838835193658	9.019210746514815
14	11.242591761675175	54.75825019186493	23.96631146390774	10.032846582552159
15	8.835228306270128	22.139566792382226	58.41425153621873	10.610953365128918
16	10.702338240109212	16.94197623473526	56.31846286309246	16.037222662063062
17	10.910141382362978	17.03899145746316	28.134068108151183	43.91679905202268
18	17.880165410954753	21.3262847154249	41.887101999379794	18.906447874240552
19	30.407862390836222	22.094004285993947	26.85433337548876	20.643799947681078
20	34.595022079625245	20.18557626176093	26.54535721524733	18.674044443366498
21	17.824295037151632	31.363808817644294	31.51219013597723	19.299706009226842
22	19.462119886561485	23.1208930944218	24.26723189583337	33.14975512318335
23	13.804140124629912	33.074568325569224	23.72108816431607	29.40020338548479
24	15.040131386330208	21.166469460127608	48.604799828144465	15.18859932539772
25	11.708438072238229	23.08728424940535	45.38025636272606	19.824021315630365
26	11.182996696285183	41.330996883385964	28.417751013895696	19.06825540643315
27	12.266881948065672	45.820809418792194	27.340015834270282	14.572292798871853
28	10.19144914756535	32.244221963899946	42.44138807994747	15.122940808587233
29	10.305008930597735	24.804713553892825	41.15515679219166	23.73512072331778
30	14.36587558837134	38.485245890279245	31.13218497338145	16.016693547967964
31	32.74211794625703	28.79316527755882	24.1652792912345	14.299437484949648
32	33.90231606520116	24.51921161272216	27.706854645210132	13.871617676866546
33	31.97855617094775	28.244249682535006	22.522950163626568	17.25424398289067
34	17.988268087708693	28.481590495280035	24.93507775943343	28.59506365757784
35	16.73200757411703	25.59062347872335	34.6599876305591	23.01738131660052
36	36.35870685637762	22.107084016915294	26.411354938854426	15.122854187852655
37	17.64247811527141	36.5149706615572	32.21165256769844	13.63089865547296
38	18.201961439913795	37.39330491018296	21.864979063768452	22.53975458613479
39	19.064963818519168	34.878791606104336	28.86947814472246	17.18676643065404
40	26.899982502611614	24.025646667093994	25.043180436187374	24.031190394107018
41	14.366135450575074	22.103705808266735	29.59484017608263	33.93531856507556
42	20.557265833837178	20.485890348544512	27.05945127497059	31.89739254264772
43	20.419712107326554	20.415294449863055	29.84387478799575	29.321118654814644
44	13.651081286629744	30.032534747907675	33.25846414507934	23.057919820383244
45	12.765644137768545	46.066032718383866	22.085515454005254	19.082807689842333
46	22.378380157615087	36.163896824310626	23.65057888636919	17.807144131705094
47	17.912821427890837	29.228521089550245	25.35553480507736	27.503122677481556
48	21.480209760770855	24.30647108859742	35.678647469202	18.534671681429728
49	21.748820658698715	22.564528116224235	36.49773313537608	19.18891808970097
50	19.48975190089202	37.21296054079057	26.85190799492056	16.445379563396852
51	16.110417182781877	37.39607677368947	23.7157176787722	22.77778836475645
52	18.69734542096811	26.3136467502499	37.529039601267435	17.45996822751456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8639.0
1	6729.0
2	4819.0
3	6286.5
4	7754.0
5	5320.0
6	2886.0
7	3129.0
8	3372.0
9	3839.5
10	4307.0
11	4434.0
12	4561.0
13	4685.0
14	4559.0
15	4309.0
16	3803.5
17	3298.0
18	3053.0
19	2808.0
20	3132.0
21	3456.0
22	3628.5
23	3801.0
24	4324.5
25	4848.0
26	5362.5
27	5877.0
28	6234.0
29	6591.0
30	9534.5
31	12478.0
32	14900.0
33	17322.0
34	22405.0
35	27488.0
36	30083.0
37	32678.0
38	37181.5
39	63570.0
40	85455.0
41	118910.0
42	152365.0
43	158292.5
44	164220.0
45	160367.5
46	156515.0
47	136683.0
48	116851.0
49	101706.5
50	86562.0
51	73807.0
52	61052.0
53	51178.5
54	41305.0
55	34073.0
56	26841.0
57	21924.0
58	17007.0
59	14452.0
60	11897.0
61	10313.0
62	8729.0
63	7610.0
64	5246.5
65	4002.0
66	3407.5
67	2813.0
68	2108.0
69	1403.0
70	1294.5
71	1186.0
72	928.5
73	671.0
74	669.0
75	667.0
76	477.5
77	288.0
78	196.5
79	105.0
80	123.0
81	141.0
82	95.5
83	50.0
84	35.5
85	21.0
86	23.0
87	25.0
88	13.5
89	2.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1154458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.842284327953514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15188646552868	10.857410980475512
2	11.577533834527548	3.436740979771196
3	4.4240089885325125	1.9698719783166476
4	2.2008796827351174	1.3066432809108297
5	1.2954864008379614	0.961398875211709
6	0.8385272283708889	0.7467395724126925
7	0.5457407538604461	0.5670027606703905
8	0.42659034147585717	0.5065260111794763
9	0.3309541281239627	0.44209037422132297
>10	2.7431526048029795	9.217860221604985
>50	0.9540782070287909	10.170811818787694
>100	1.4624898883403836	53.49828913267427
>500	0.04398024924840768	4.249950718918714
>1k	0.004104823263184718	1.376729888835884
>5k	5.864033233121024E-4	0.6919334060086786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7950	0.6886348398988963	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4333	0.3753276429285431	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT	2692	0.23318301748526146	Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2459	0.21300038632847623	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	2239	0.19394382472121116	Illumina Single End Adapter 2 (95% over 21bp)
GTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1408	0.12196199428649636	No Hit
AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	1375	0.11910351004540659	No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	1312	0.1136464037669625	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24175847020853075	0.0
2	0.0	0.0	0.0	0.7182591311247356	0.0
3	0.0	0.0	0.0	0.9571591170921766	0.0
4	8.662073457847752E-5	0.0	0.0	1.2495907170291167	0.0
5	8.662073457847752E-5	0.0	0.0	2.1021986074850707	0.0
6	8.662073457847752E-5	0.0	0.0	2.7101895434914045	0.0
7	8.662073457847752E-5	0.0	0.0	3.085603807154526	0.0
8	8.662073457847752E-5	0.0	0.0	3.894641468117506	0.0
9	8.662073457847752E-5	0.0	0.0	4.158921329316441	0.0
10	8.662073457847752E-5	0.0	0.0	4.653785585963283	0.0
11	8.662073457847752E-5	0.0	0.0	5.306646062481268	0.0
12	8.662073457847752E-5	0.0	0.0	5.807660391283182	0.0
13	1.7324146915695504E-4	0.0	0.0	6.0126050492958605	0.0
14	1.7324146915695504E-4	0.0	0.0	6.074365633050315	0.0
15	1.7324146915695504E-4	0.0	0.0	6.207328460628277	0.0
16	1.7324146915695504E-4	0.0	0.0	6.513359515894039	0.0
17	1.7324146915695504E-4	0.0	0.0	6.869630597215316	0.0
18	3.464829383139101E-4	0.0	0.0	7.468872839029224	0.0
19	3.464829383139101E-4	0.0	0.0	7.675896394671786	0.0
20	3.464829383139101E-4	0.0	0.0	7.8817938807648265	0.0
21	3.464829383139101E-4	0.0	0.0	8.198912390056632	0.0
22	3.464829383139101E-4	0.0	0.0	8.47783115539933	0.0
23	3.464829383139101E-4	0.0	0.0	8.877672466213582	0.0
24	7.795866112062977E-4	0.0	0.0	9.111375208106315	0.0
25	7.795866112062977E-4	0.0	0.0	9.301507720506073	0.0
26	7.795866112062977E-4	0.0	0.0	9.508098172475743	0.0
27	7.795866112062977E-4	0.0	0.0	9.690694680967173	0.0
28	7.795866112062977E-4	0.0	0.0	9.915821970136635	0.0
29	7.795866112062977E-4	0.0	0.0	10.129861805280054	0.0
30	7.795866112062977E-4	0.0	0.0	10.415363746450716	0.0
31	7.795866112062977E-4	0.0	0.0	10.699912859541014	0.0
32	8.662073457847752E-4	0.0	0.0	10.914212556888167	0.0
33	8.662073457847752E-4	0.0	0.0	11.15302592212103	0.0
34	8.662073457847752E-4	0.0	0.0	11.382657489488574	0.0
35	8.662073457847752E-4	0.0	0.0	11.736849673179968	0.0
36	8.662073457847752E-4	0.0	0.0	11.976702487227772	0.0
37	8.662073457847752E-4	0.0	0.0	12.211358057200869	0.0
38	9.528280803632527E-4	0.0	0.0	12.42687044483212	0.0
39	9.528280803632527E-4	0.0	0.0	12.643162419074578	0.0
40	9.528280803632527E-4	0.0	0.0	12.917143802546303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.092282E-10	46.000004	25
ATTACTA	45	3.092282E-10	46.000004	28
CACGCAC	45	3.092282E-10	46.000004	45
ATTCGCG	90	0.0	46.000004	1
CAATTAC	45	3.092282E-10	46.000004	26
TAAGTAC	45	3.092282E-10	46.000004	24
CACGACG	45	3.092282E-10	46.000004	26
GTTACCA	45	3.092282E-10	46.000004	12
CGTAATA	45	3.092282E-10	46.000004	33
CGGTCTA	45	3.092282E-10	46.000004	31
GAACCAC	45	3.092282E-10	46.000004	38
GTAAGTA	45	3.092282E-10	46.000004	23
ATACGAG	45	3.092282E-10	46.000004	1
TGCAGAT	45	3.092282E-10	46.000004	40
TCACGGT	20	6.3114666E-4	46.0	33
CCTTCGC	50	1.6370905E-11	46.0	27
GTTTGCG	60	0.0	46.0	1
CTTCGCA	20	6.3114666E-4	46.0	14
AAGTATG	80	0.0	46.0	1
AGCGTAT	30	1.86141E-6	46.0	33
>>END_MODULE