FastQCFastQC Report
Thu 26 May 2016
SRR1544738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544738_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1905996
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92730.48651728545075645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57540.3018894058539472No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT20760.10891943110058994TruSeq Adapter, Index 14 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTC206.3125044E-446.00000429
CAAGTCG206.3125044E-446.00000431
TTTCGCG206.3125044E-446.0000041
ATATACG351.0200529E-746.0000041
TACGAAC700.046.00000418
TACTCGC405.6152203E-946.00000445
CGTCGTC351.0200529E-746.00000430
CGCGTAA405.6152203E-946.00000431
CAATCCG206.3125044E-446.00000413
ATGTTCG700.046.00000411
GCGTAAC405.6152203E-946.00000432
AATCGGC206.3125044E-446.00000413
TACCGTT206.3125044E-446.00000423
TCCGCGA253.418009E-546.029
GTCGATG301.862054E-646.01
GTCGAAC750.046.023
GAATCGT301.862054E-646.013
CCTCGAA253.418009E-546.042
TACGCCC301.862054E-646.01
CGGATTA301.862054E-646.017
Produced by FastQC (version 0.11.5)