##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544738_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1905996
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.756040411417445	33.0	31.0	34.0	31.0	34.0
2	33.16869028056722	34.0	33.0	34.0	31.0	34.0
3	33.19902297801254	34.0	33.0	34.0	31.0	34.0
4	36.50402466741798	37.0	37.0	37.0	35.0	37.0
5	36.50355876927339	37.0	37.0	37.0	35.0	37.0
6	36.567439805749856	37.0	37.0	37.0	35.0	37.0
7	36.65820232571317	37.0	37.0	37.0	35.0	37.0
8	36.66544001141661	37.0	37.0	37.0	35.0	37.0
9	38.37423845590442	39.0	39.0	39.0	37.0	39.0
10	38.276153255305886	39.0	39.0	39.0	37.0	39.0
11	38.139740062413566	39.0	39.0	39.0	35.0	39.0
12	37.574940870809804	39.0	35.0	39.0	35.0	39.0
13	37.45781890413201	39.0	35.0	39.0	35.0	39.0
14	38.661073265631195	41.0	36.0	41.0	35.0	41.0
15	38.70043011632763	41.0	36.0	41.0	35.0	41.0
16	38.77241295364733	41.0	36.0	41.0	35.0	41.0
17	38.749292233561874	41.0	36.0	41.0	35.0	41.0
18	38.74041813309157	40.0	36.0	41.0	35.0	41.0
19	38.72875284103429	40.0	36.0	41.0	35.0	41.0
20	38.617709585959254	40.0	36.0	41.0	35.0	41.0
21	38.54465644209117	40.0	35.0	41.0	35.0	41.0
22	38.493484246556655	40.0	35.0	41.0	35.0	41.0
23	38.416645680263755	40.0	35.0	41.0	35.0	41.0
24	38.359059515340014	40.0	35.0	41.0	35.0	41.0
25	38.3557090361155	40.0	35.0	41.0	35.0	41.0
26	38.30114858583124	40.0	35.0	41.0	35.0	41.0
27	38.279949170932156	40.0	35.0	41.0	35.0	41.0
28	38.23779798068831	40.0	35.0	41.0	35.0	41.0
29	38.21370139286756	40.0	36.0	41.0	35.0	41.0
30	38.169051246697265	40.0	36.0	41.0	35.0	41.0
31	38.06768010006317	40.0	35.0	41.0	35.0	41.0
32	37.94196787401442	40.0	35.0	41.0	35.0	41.0
33	37.799373660805166	40.0	35.0	41.0	35.0	41.0
34	37.651364955645235	40.0	35.0	41.0	35.0	41.0
35	37.51168522914004	40.0	35.0	41.0	34.0	41.0
36	37.44964784815918	40.0	35.0	41.0	34.0	41.0
37	37.41857275671093	40.0	35.0	41.0	34.0	41.0
38	37.2998232944875	40.0	35.0	41.0	33.0	41.0
39	37.310613453543446	40.0	35.0	41.0	34.0	41.0
40	37.218387131977195	40.0	35.0	41.0	33.0	41.0
41	37.1945780578763	40.0	35.0	41.0	34.0	41.0
42	37.18353920994588	40.0	35.0	41.0	34.0	41.0
43	37.12540477524612	40.0	35.0	41.0	34.0	41.0
44	37.02579228917584	40.0	35.0	41.0	33.0	41.0
45	36.95302193708696	39.0	35.0	41.0	33.0	41.0
46	36.89899349211646	39.0	35.0	41.0	33.0	41.0
47	36.83603008610721	39.0	35.0	41.0	33.0	41.0
48	36.782492198304716	39.0	35.0	41.0	33.0	41.0
49	36.740309528456514	39.0	35.0	41.0	33.0	41.0
50	36.642913206533485	39.0	35.0	41.0	33.0	41.0
51	36.55760767598673	39.0	35.0	41.0	33.0	41.0
52	36.30941565459739	38.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	4.0
11	3.0
12	5.0
13	10.0
14	7.0
15	24.0
16	46.0
17	92.0
18	187.0
19	415.0
20	691.0
21	1202.0
22	2006.0
23	3304.0
24	6075.0
25	10178.0
26	13967.0
27	15531.0
28	15424.0
29	15114.0
30	15953.0
31	18947.0
32	23838.0
33	33631.0
34	86460.0
35	333904.0
36	73798.0
37	96016.0
38	195107.0
39	941145.0
40	2911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.181440044994847	24.692968925433213	29.651793602924663	14.473797426647275
2	31.134325570462895	26.058501696750675	29.13290479098592	13.674267941800508
3	28.51705879760503	24.695854555833275	32.49392968295841	14.293156963603282
4	24.80020944430104	27.610970851985	31.693665674009807	15.895154029704155
5	22.47092858536954	33.65951450055509	29.226084419904343	14.643472494171025
6	22.314842213729726	41.89998300101364	25.351941976793235	10.433232808463396
7	88.38843313417237	3.841613518601298	6.191408586376887	1.5785447608494456
8	89.39992528840564	2.716742322649156	6.0735174680324615	1.8098149209127408
9	83.77735315289225	5.516276004776506	7.489312674318309	3.217058168012944
10	40.73135515499508	34.60642099983421	13.666135710673055	10.996088134497658
11	38.63901078491245	22.531894085821797	24.58578087257266	14.243314256693088
12	37.32069742014149	20.612687539743	27.043078789252444	15.023536250863065
13	22.713269072967623	34.25180325667	27.99460229717166	15.040325373190711
14	15.30029443923282	37.81791777107612	31.038994835246243	15.842792954444814
15	14.793630206988892	25.468154182904897	44.668876534893045	15.06933907521317
16	18.51079435633653	22.60267072963427	42.125954094342276	16.760580819686925
17	18.6336172793647	21.43708591203759	30.903107876406878	29.026188932190834
18	19.37784759254479	24.39480460609571	37.1786194724438	19.048728328915697
19	25.13132241620654	24.212800026862595	30.6567275062487	19.999150050682164
20	27.285366810843254	24.809915655646705	29.759086587799764	18.145630945710277
21	21.73713900763695	27.445178268999516	32.56328974457449	18.254392978789042
22	20.94406284168487	23.866157116804025	29.717533510038844	25.47224653147226
23	16.92910163505065	29.354311341681726	29.313964982088102	24.40262204117952
24	16.978419681888106	25.471249677334058	40.08738738171539	17.462943259062452
25	16.73649892234821	27.08116911053329	35.842152869156074	20.34017909796243
26	16.782039416661945	33.21108753638518	29.90394523388297	20.10292781306991
27	16.250716160999286	35.58239366714306	31.034954952686157	17.1319352191715
28	15.168919557019006	30.677556511136434	36.558576198481	17.594947733363554
29	16.687862933605317	25.242917613678095	36.23066365301921	21.838555799697374
30	17.66955439570702	30.745342592534296	33.76271513686282	17.822387874895853
31	24.847009122789345	28.078652840824432	30.095708490469026	16.9786295459172
32	26.31831336477096	26.327285052014798	30.681438995674704	16.672962587539534
33	27.127076866897937	27.364118287761357	27.729701426445807	17.7791034188949
34	20.666622595220556	27.521831105626664	28.97529690513516	22.836249394017617
35	19.24311488586545	27.210865080514335	33.09156997181526	20.45445006180496
36	27.616060054690568	25.40246674179799	29.25924293650144	17.72223026701
37	19.308907258986903	32.106835481291675	31.71433728087572	16.869919978845708
38	19.454710293201035	32.39225056086162	27.517109164972016	20.635929980965333
39	19.450722876648218	31.774725655247966	29.57587529039935	19.198676177704467
40	22.834832811821222	26.742133771529424	29.505151112594152	20.9178823040552
41	17.23775915584293	26.284735120115677	31.78820941911735	24.689296304924042
42	20.274071928797333	25.250420252718264	29.35808889420544	25.11741892427896
43	19.77784843200091	25.587147087402073	30.539151183947922	24.0958532966491
44	17.290907221211377	29.493293794950254	31.39146147211222	21.824337511726153
45	16.84625780956518	35.26890927368158	28.146333990207744	19.738498926545493
46	20.325016421860276	32.18254392978789	29.411919017668453	18.08052063068338
47	18.47139238487384	29.629967743898728	29.62744937554958	22.27119049567785
48	20.454817323855874	27.152155618374856	32.94534720954294	19.447679848226336
49	21.155868113049557	26.25886937852965	33.783911403801476	18.801351104619318
50	18.799409862350185	32.42677319364783	30.206779027867846	18.567037916134137
51	18.423385988218232	32.29450638931037	27.839145517619134	21.442962104852267
52	19.317196888136177	27.935578038988538	33.99797271347894	18.749252359396348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6428.0
1	6375.5
2	6323.0
3	11532.5
4	16742.0
5	11385.0
6	6028.0
7	6406.5
8	6785.0
9	6910.0
10	7035.0
11	6964.0
12	6893.0
13	7231.5
14	7214.0
15	6858.0
16	6783.5
17	6709.0
18	6835.0
19	6961.0
20	7765.5
21	8570.0
22	10611.5
23	12653.0
24	13952.5
25	15252.0
26	17612.5
27	19973.0
28	25538.0
29	31103.0
30	34196.5
31	37290.0
32	46196.0
33	55102.0
34	62757.0
35	70412.0
36	74117.0
37	77822.0
38	90227.5
39	119246.0
40	135859.0
41	157521.5
42	179184.0
43	185402.5
44	191621.0
45	187481.0
46	183341.0
47	171257.0
48	159173.0
49	150972.0
50	142771.0
51	128361.5
52	113952.0
53	95533.0
54	77114.0
55	66209.0
56	55304.0
57	49591.0
58	43878.0
59	38330.0
60	32782.0
61	28146.0
62	23510.0
63	21025.0
64	14523.0
65	10506.0
66	9387.5
67	8269.0
68	6875.0
69	5481.0
70	4768.5
71	4056.0
72	3125.0
73	2194.0
74	1947.5
75	1701.0
76	1196.5
77	692.0
78	518.5
79	345.0
80	371.0
81	397.0
82	273.5
83	150.0
84	82.0
85	14.0
86	10.5
87	7.0
88	5.0
89	4.0
90	5.0
91	3.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1905996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.859786815789002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3448014073568	11.196076865912813
2	10.114790241559428	3.006072533519921
3	3.5130310663746833	1.5660867817071513
4	1.769884655601423	1.0520043468309315
5	1.0751718043160305	0.7988411901241712
6	0.757115119093425	0.6750341558843399
7	0.5058421172087652	0.5261694216918722
8	0.4131347475195324	0.4911275419468053
9	0.32600984788739407	0.435999315550903
>10	3.455069550942237	12.237340701044012
>50	1.0817414516553279	11.35464508347294
>100	1.6199459685572273	53.68088669062158
>500	0.021329110843413032	1.8791202474333455
>1k	0.0014219407228942022	0.3068037668201408
>5k	7.109703614471011E-4	0.7937913574390937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9273	0.48651728545075645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5754	0.3018894058539472	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2076	0.10891943110058994	TruSeq Adapter, Index 14 (96% over 26bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05598122976123769	0.0
2	0.0	0.0	0.0	0.24700996224546118	0.0
3	0.0	0.0	0.0	0.3633795663789431	0.0
4	0.0	0.0	0.0	0.5206726561860571	0.0
5	0.0	0.0	0.0	0.9428666167190277	0.0
6	0.0	0.0	0.0	1.2492680991985292	0.0
7	0.0	0.0	0.0	1.4394049095590966	0.0
8	0.0	0.0	0.0	1.8229838887384864	0.0
9	0.0	0.0	0.0	1.9655864965089118	0.0
10	0.0	0.0	0.0	2.278808559934019	0.0
11	0.0	0.0	0.0	2.7386206476823665	0.0
12	0.0	0.0	0.0	3.092031672679271	0.0
13	0.0	0.0	0.0	3.2206783225148428	0.0
14	0.0	0.0	0.0	3.2728295337450866	0.0
15	5.246600727388725E-5	0.0	0.0	3.355568427216007	0.0
16	5.246600727388725E-5	0.0	0.0	3.5420850830746757	0.0
17	5.246600727388725E-5	0.0	0.0	3.764173691865041	0.0
18	5.246600727388725E-5	0.0	0.0	4.052369469820503	0.0
19	1.049320145477745E-4	0.0	0.0	4.198644698100101	0.0
20	1.049320145477745E-4	0.0	0.0	4.3737762303803365	0.0
21	1.049320145477745E-4	0.0	0.0	4.592716878734268	0.0
22	2.09864029095549E-4	0.0	0.0	4.814175895437346	0.0
23	2.09864029095549E-4	0.0	0.0	5.0578280332172785	0.0
24	2.6233003636943623E-4	0.0	0.0	5.240724534574049	0.0
25	2.6233003636943623E-4	0.0	0.0	5.399801468628476	0.0
26	2.6233003636943623E-4	0.0	0.0	5.547755609140838	0.0
27	2.6233003636943623E-4	0.0	0.0	5.708616387442576	0.0
28	3.147960436433235E-4	0.0	0.0	5.873726912333499	0.0
29	3.147960436433235E-4	0.0	0.0	6.055574093544792	0.0
30	3.147960436433235E-4	0.0	0.0	6.274357343876902	0.0
31	3.6726205091721075E-4	0.0	0.0	6.4691636288848455	0.0
32	3.6726205091721075E-4	0.0	0.0	6.648282577717897	0.0
33	3.6726205091721075E-4	0.0	0.0	6.828136050652782	0.0
34	3.6726205091721075E-4	0.0	0.0	7.024201519835299	0.0
35	3.6726205091721075E-4	0.0	0.0	7.261715134764186	0.0
36	6.820580945605342E-4	0.0	0.0	7.455839361677569	0.0
37	6.820580945605342E-4	0.0	0.0	7.667802031064074	0.0
38	7.345241018344215E-4	0.0	0.0	7.870583149177648	0.0
39	7.345241018344215E-4	0.0	0.0	8.150436831976563	0.0
40	7.345241018344215E-4	0.0	0.0	8.377457245450673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTC	20	6.3125044E-4	46.000004	29
CAAGTCG	20	6.3125044E-4	46.000004	31
TTTCGCG	20	6.3125044E-4	46.000004	1
ATATACG	35	1.0200529E-7	46.000004	1
TACGAAC	70	0.0	46.000004	18
TACTCGC	40	5.6152203E-9	46.000004	45
CGTCGTC	35	1.0200529E-7	46.000004	30
CGCGTAA	40	5.6152203E-9	46.000004	31
CAATCCG	20	6.3125044E-4	46.000004	13
ATGTTCG	70	0.0	46.000004	11
GCGTAAC	40	5.6152203E-9	46.000004	32
AATCGGC	20	6.3125044E-4	46.000004	13
TACCGTT	20	6.3125044E-4	46.000004	23
TCCGCGA	25	3.418009E-5	46.0	29
GTCGATG	30	1.862054E-6	46.0	1
GTCGAAC	75	0.0	46.0	23
GAATCGT	30	1.862054E-6	46.0	13
CCTCGAA	25	3.418009E-5	46.0	42
TACGCCC	30	1.862054E-6	46.0	1
CGGATTA	30	1.862054E-6	46.0	17
>>END_MODULE