FastQCFastQC Report
Thu 26 May 2016
SRR1544737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544737_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2051986
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80730.3934237368091205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64270.31320876458221447No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT28460.13869490337653376Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT26340.12836344887343287Illumina Single End Adapter 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA301.8621231E-646.029
AATCCCG551.8189894E-1246.036
CGAACTA253.418101E-546.027
AAATCCG800.046.025
TCCGCTA253.418101E-546.041
CAACGTA453.110472E-1046.025
CTCCGCG253.418101E-546.01
CGAGTCT453.110472E-1046.026
CGAGTCA301.8621231E-646.014
CGACGTG253.418101E-546.032
CAACGAA206.312619E-446.040
GCGAATG800.046.01
CGTGCGG453.110472E-1046.02
TTAGCCG206.312619E-446.021
TCCGAGT206.312619E-446.033
GGCACGA453.110472E-1046.033
CGCATCA206.312619E-446.040
GACCGGT253.418101E-546.09
TAGCGCG750.046.01
TGGAACG301.8621231E-646.01
Produced by FastQC (version 0.11.5)