##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544737_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2051986 Sequences flagged as poor quality 0 Sequence length 52 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69826402324382 33.0 31.0 34.0 31.0 34.0 2 33.11357387428569 34.0 33.0 34.0 31.0 34.0 3 33.15253710307965 34.0 33.0 34.0 31.0 34.0 4 36.468808266723066 37.0 37.0 37.0 35.0 37.0 5 36.462788245143976 37.0 37.0 37.0 35.0 37.0 6 36.53263375091253 37.0 37.0 37.0 35.0 37.0 7 36.638632524783304 37.0 37.0 37.0 35.0 37.0 8 36.63867735939719 37.0 37.0 37.0 35.0 37.0 9 38.368684289269034 39.0 39.0 39.0 37.0 39.0 10 38.240190235216026 39.0 39.0 39.0 37.0 39.0 11 38.06636497519963 39.0 39.0 39.0 35.0 39.0 12 37.518723324623075 39.0 35.0 39.0 35.0 39.0 13 37.43925056018901 39.0 35.0 39.0 35.0 39.0 14 38.65960683942288 41.0 36.0 41.0 35.0 41.0 15 38.697813240441214 41.0 36.0 41.0 35.0 41.0 16 38.753315568429805 41.0 36.0 41.0 35.0 41.0 17 38.71029480707958 41.0 36.0 41.0 35.0 41.0 18 38.73141337221599 40.0 36.0 41.0 35.0 41.0 19 38.726982055433126 40.0 36.0 41.0 35.0 41.0 20 38.59468631852264 40.0 36.0 41.0 35.0 41.0 21 38.528891035318956 40.0 35.0 41.0 35.0 41.0 22 38.46812161486482 40.0 35.0 41.0 35.0 41.0 23 38.411431656941126 40.0 35.0 41.0 35.0 41.0 24 38.36596789646713 40.0 35.0 41.0 35.0 41.0 25 38.36783048227424 40.0 35.0 41.0 35.0 41.0 26 38.30010584867538 40.0 35.0 41.0 35.0 41.0 27 38.28675975372152 40.0 35.0 41.0 35.0 41.0 28 38.274561327416464 40.0 36.0 41.0 35.0 41.0 29 38.25306069339654 40.0 36.0 41.0 35.0 41.0 30 38.189161134627625 40.0 36.0 41.0 35.0 41.0 31 38.10031549922855 40.0 35.0 41.0 35.0 41.0 32 38.0092861257338 40.0 35.0 41.0 35.0 41.0 33 37.89200803514254 40.0 35.0 41.0 35.0 41.0 34 37.759895535349656 40.0 35.0 41.0 35.0 41.0 35 37.650559506741274 40.0 35.0 41.0 34.0 41.0 36 37.57678658626326 40.0 35.0 41.0 34.0 41.0 37 37.5581222289041 40.0 35.0 41.0 34.0 41.0 38 37.44039384284298 40.0 35.0 41.0 34.0 41.0 39 37.453821322367695 40.0 35.0 41.0 34.0 41.0 40 37.385949026942676 40.0 35.0 41.0 34.0 41.0 41 37.358234412905354 40.0 35.0 41.0 34.0 41.0 42 37.332713770951656 40.0 35.0 41.0 34.0 41.0 43 37.29544548549551 40.0 35.0 41.0 34.0 41.0 44 37.22435240786243 40.0 35.0 41.0 34.0 41.0 45 37.137498014119004 40.0 35.0 41.0 34.0 41.0 46 37.08124568101342 40.0 35.0 41.0 34.0 41.0 47 37.035528020171675 40.0 35.0 41.0 33.0 41.0 48 36.97988972634316 39.0 35.0 41.0 33.0 41.0 49 36.95739542082646 39.0 35.0 41.0 33.0 41.0 50 36.85112617727411 39.0 35.0 41.0 33.0 41.0 51 36.778471198146576 39.0 35.0 41.0 33.0 41.0 52 36.581146265130464 39.0 35.0 41.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 4.0 12 4.0 13 4.0 14 12.0 15 20.0 16 52.0 17 104.0 18 218.0 19 463.0 20 840.0 21 1306.0 22 2276.0 23 3726.0 24 6293.0 25 10098.0 26 13794.0 27 15386.0 28 15119.0 29 15644.0 30 16748.0 31 20163.0 32 25531.0 33 35424.0 34 87383.0 35 362489.0 36 80534.0 37 97993.0 38 201842.0 39 1034748.0 40 3765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.23974237640997 23.305519628301557 30.307029385190738 14.147708610097729 2 30.157369494723646 25.321517788133058 31.033593796448905 13.487518920694392 3 28.606140587703816 25.645545339977954 32.120686983244525 13.627627089073707 4 25.41294141383031 28.601852059419507 31.225310504067767 14.759896022682417 5 22.962096232625367 34.607009989346906 28.05657543472519 14.374318343302535 6 22.010140420061344 42.959698555448234 24.816446116104107 10.213714908386315 7 89.34953747247788 3.6328220562908324 5.3086132166593725 1.7090272545719123 8 89.7250760970104 2.9076221767594905 5.47508608733198 1.892215638898121 9 84.92621294687196 5.137267018390964 6.99926802619511 2.9372520085419684 10 41.60174582087792 36.476613388200505 12.201642701265992 9.719998089655583 11 36.148151108243425 21.884067435157938 27.885521636112525 14.082259820486104 12 35.565983393648885 22.239625416547675 27.83761682584579 14.35677436395765 13 21.145660837841973 37.42354967334085 26.66728720371386 14.76350228510331 14 13.77718951298888 40.271765986707514 29.491331812205345 16.459712688098264 15 13.51290895746852 27.971146001970776 44.251861367475215 14.264083673085489 16 15.988803042515883 24.94329883342284 42.41651746162011 16.651380662441166 17 16.034661055192384 22.901325837505716 31.289882094712144 29.774131012589756 18 17.991789417666592 24.246754120154815 38.41931670099113 19.342139761187454 19 25.02453720444486 26.532296029310142 28.810771613451553 19.632395152793443 20 27.008956201455565 25.459335492542344 30.616631887352057 16.91507641865003 21 20.014853902512005 29.562579861655973 31.514445030326716 18.908121205505303 22 19.628155357785094 25.65870332448662 28.494931251967603 26.218210065760683 23 16.574089686771742 31.100163451407564 28.414862479568576 23.91088438225212 24 15.207267495977067 25.198709932718838 41.48878208720722 18.10524048409687 25 15.904689408212336 28.590351006293414 37.2118523225792 18.293107262915047 26 15.781735352970244 35.89897786827005 29.047761534435416 19.271525244324277 27 14.764866816830136 37.401863365539526 29.88071068710995 17.952559130520385 28 13.216659372919699 30.74275360553142 38.51229979151904 17.528287230029836 29 14.894546064154435 25.989163668757975 37.16009758351178 21.956192683575814 30 16.492705115921844 33.95057276219233 31.93554926788 17.621172854005827 31 24.371803706263105 30.029249712230005 28.923491680742465 16.67545490076443 32 26.30578376265725 26.222108727837323 30.45542221048292 17.01668529902251 33 24.79592940692578 29.328562670505548 27.910814206334738 17.96469371623393 34 17.77960473414536 28.012715486362964 30.186219594090797 24.021460185400876 35 18.855732933850426 27.719633564751415 33.33307342252822 20.091560078869932 36 26.273522333973037 28.82544032951492 27.540538775605683 17.36049856090636 37 17.319952475309286 34.530352546264936 31.616589976734733 16.533105001691045 38 17.81951728715498 33.16426135460963 29.926519966510494 19.089701391724894 39 18.15065989728975 33.78507455703888 29.940652616538323 18.123612929133046 40 22.47934440098519 27.352135930751963 29.480513024942667 20.68800664332018 41 17.23062438047823 26.10665959709277 31.091293995183207 25.5714220272458 42 18.706560376142917 25.312940731564442 29.145033153247635 26.835465739045006 43 18.75193105605984 26.115139187109467 30.76283171522613 24.370098041604574 44 16.794364094102008 29.25341595897828 32.68038865762242 21.271831289297296 45 15.882808167307182 37.228177970025136 27.42348144675451 19.46553241591317 46 18.788724679408144 34.45759376525961 28.014518617573415 18.739162937758834 47 17.840082729609268 29.253757091909982 30.069698331275163 22.836461847205587 48 18.724104355487807 26.57698444336365 35.0233383658563 19.67557283529225 49 20.099942202334713 26.397645987838125 34.930257808776474 18.572154001050688 50 17.957919790875764 33.41972118718159 30.76405004712508 17.858308974817565 51 16.646799734501112 32.60022241867147 28.929875739892964 21.823102106934453 52 17.89909872679443 28.39527170263345 35.69824550459896 18.00738406597316 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7282.0 1 6485.0 2 5688.0 3 10028.5 4 14369.0 5 9764.0 6 5159.0 7 5330.0 8 5501.0 9 6051.5 10 6602.0 11 6817.5 12 7033.0 13 6484.5 14 6195.5 15 6455.0 16 6755.5 17 7056.0 18 7031.0 19 7006.0 20 7859.5 21 8713.0 22 12542.0 23 16371.0 24 19401.5 25 22432.0 26 24853.5 27 27275.0 28 32879.5 29 38484.0 30 42383.5 31 46283.0 32 56643.5 33 67004.0 34 77553.0 35 88102.0 36 95736.5 37 103371.0 38 110206.0 39 135290.5 40 153540.0 41 184811.5 42 216083.0 43 222606.0 44 229129.0 45 227197.5 46 225266.0 47 200640.5 48 176015.0 49 153819.5 50 131624.0 51 115316.5 52 99009.0 53 82425.0 54 65841.0 55 56576.5 56 47312.0 57 38792.5 58 30273.0 59 26850.5 60 23428.0 61 19181.5 62 14935.0 63 12785.5 64 8336.0 65 6036.0 66 4927.0 67 3818.0 68 3183.0 69 2548.0 70 1986.5 71 1425.0 72 1130.0 73 835.0 74 716.0 75 597.0 76 434.0 77 271.0 78 190.0 79 109.0 80 77.5 81 46.0 82 28.5 83 11.0 84 18.0 85 25.0 86 16.5 87 8.0 88 4.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 2051986.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.848929127831411 #Duplication Level Percentage of deduplicated Percentage of total 1 74.17830316867041 10.27290063405709 2 10.924109750772999 3.0257444344621467 3 4.092104768325787 1.700138067606144 4 2.0158047540909725 1.11666948699806 5 1.1562818603624059 0.8006632767978007 6 0.7423871850802528 0.6168760506952009 7 0.5858582672240727 0.567945673521821 8 0.40950517507947076 0.4536966517724627 9 0.309814844196479 0.3861543438024452 >10 2.8539202573363256 9.087616627799495 >50 0.9459473249568658 9.457771073154788 >100 1.7391967107566413 57.59284879198551 >500 0.042868772051650524 3.512760118497675 >1k 0.0031885863509492126 0.6967721658628584 >5k 7.085747446553806E-4 0.7114426029865113 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8073 0.3934237368091205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6427 0.31320876458221447 No Hit CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT 2846 0.13869490337653376 Illumina Single End Adapter 2 (95% over 22bp) CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT 2634 0.12836344887343287 Illumina Single End Adapter 2 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.13294437681348703 0.0 2 0.0 0.0 0.0 0.4234434347992628 0.0 3 0.0 0.0 0.0 0.5966902308300349 0.0 4 0.0 0.0 0.0 0.7972763946732581 0.0 5 0.0 0.0 0.0 1.3489370785180796 0.0 6 0.0 0.0 0.0 1.7269123668485067 0.0 7 0.0 0.0 0.0 1.9854911290817774 0.0 8 0.0 0.0 0.0 2.5009429888897876 0.0 9 0.0 0.0 0.0 2.691782497541406 0.0 10 0.0 0.0 0.0 3.079845573995144 0.0 11 0.0 0.0 0.0 3.596856898633811 0.0 12 0.0 0.0 0.0 3.987210439057576 0.0 13 9.746655191604621E-5 0.0 0.0 4.145252452989445 0.0 14 9.746655191604621E-5 0.0 0.0 4.198128057403901 0.0 15 9.746655191604621E-5 0.0 0.0 4.2937427448335415 0.0 16 9.746655191604621E-5 0.0 0.0 4.527857402535885 0.0 17 9.746655191604621E-5 0.0 0.0 4.806611741015777 0.0 18 9.746655191604621E-5 0.0 0.0 5.199548145065317 0.0 19 9.746655191604621E-5 0.0 0.0 5.381566930768534 0.0 20 9.746655191604621E-5 0.0 0.0 5.578887965122569 0.0 21 9.746655191604621E-5 0.0 0.0 5.832934532691744 0.0 22 9.746655191604621E-5 0.0 0.0 6.112614803414838 0.0 23 9.746655191604621E-5 0.0 0.0 6.42835769834687 0.0 24 1.461998278740693E-4 0.0 0.0 6.646439108259023 0.0 25 1.461998278740693E-4 0.0 0.0 6.833038821902294 0.0 26 1.461998278740693E-4 0.0 0.0 7.009160881214589 0.0 27 1.9493310383209242E-4 0.0 0.0 7.18810947053245 0.0 28 1.9493310383209242E-4 0.0 0.0 7.377389514353411 0.0 29 1.9493310383209242E-4 0.0 0.0 7.579340209923459 0.0 30 1.9493310383209242E-4 0.0 0.0 7.829195715760244 0.0 31 1.9493310383209242E-4 0.0 0.0 8.055707982413136 0.0 32 1.9493310383209242E-4 0.0 0.0 8.26760026627862 0.0 33 1.9493310383209242E-4 0.0 0.0 8.482903879461166 0.0 34 1.9493310383209242E-4 0.0 0.0 8.702885887135682 0.0 35 1.9493310383209242E-4 0.0 0.0 8.994700743572325 0.0 36 1.9493310383209242E-4 0.0 0.0 9.219653545394559 0.0 37 1.9493310383209242E-4 0.0 0.0 9.442705749454431 0.0 38 1.9493310383209242E-4 0.0 0.0 9.67199581283693 0.0 39 1.9493310383209242E-4 0.0 0.0 9.934814370078549 0.0 40 1.9493310383209242E-4 0.0 0.0 10.19558612972993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTA 30 1.8621231E-6 46.0 29 AATCCCG 55 1.8189894E-12 46.0 36 CGAACTA 25 3.418101E-5 46.0 27 AAATCCG 80 0.0 46.0 25 TCCGCTA 25 3.418101E-5 46.0 41 CAACGTA 45 3.110472E-10 46.0 25 CTCCGCG 25 3.418101E-5 46.0 1 CGAGTCT 45 3.110472E-10 46.0 26 CGAGTCA 30 1.8621231E-6 46.0 14 CGACGTG 25 3.418101E-5 46.0 32 CAACGAA 20 6.312619E-4 46.0 40 GCGAATG 80 0.0 46.0 1 CGTGCGG 45 3.110472E-10 46.0 2 TTAGCCG 20 6.312619E-4 46.0 21 TCCGAGT 20 6.312619E-4 46.0 33 GGCACGA 45 3.110472E-10 46.0 33 CGCATCA 20 6.312619E-4 46.0 40 GACCGGT 25 3.418101E-5 46.0 9 TAGCGCG 75 0.0 46.0 1 TGGAACG 30 1.8621231E-6 46.0 1 >>END_MODULE