FastQCFastQC Report
Thu 26 May 2016
SRR1544736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544736_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2240043
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99910.4460182237573118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41670.18602321473293146No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT24810.11075680243638181TruSeq Adapter, Index 23 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT23840.10642652841932052Illumina PCR Primer Index 7 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA253.4182012E-546.028
CGAGTCA253.4182012E-546.046
CGCGGAC206.3127436E-446.039
CTATCGT301.8621995E-646.024
GCGCGTT301.8621995E-646.026
ATTACGC253.4182012E-546.025
ATTCCCG253.4182012E-546.030
AATGCGC301.8621995E-646.023
TCGGACT206.3127436E-446.039
TCTACGA301.8621995E-646.029
GCGATCG206.3127436E-446.09
TTAACGG1200.046.02
TCCAACG253.4182012E-546.017
GTGCGTA253.4182012E-546.016
ATCGCGA206.3127436E-446.042
CCCGCGA206.3127436E-446.033
TGCGTAA253.4182012E-546.013
TGCCGAT206.3127436E-446.023
CCGTAAA351.0201438E-745.99999639
TTCGCTA453.110472E-1045.99999626
Produced by FastQC (version 0.11.5)