##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544736_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2240043
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.698362486791545	33.0	31.0	34.0	31.0	34.0
2	33.12719041554113	34.0	33.0	34.0	31.0	34.0
3	33.17002084334988	34.0	33.0	34.0	31.0	34.0
4	36.488584370925025	37.0	37.0	37.0	35.0	37.0
5	36.485034439071036	37.0	37.0	37.0	35.0	37.0
6	36.55094299529072	37.0	37.0	37.0	35.0	37.0
7	36.63864443673626	37.0	37.0	37.0	35.0	37.0
8	36.62816249509496	37.0	37.0	37.0	35.0	37.0
9	38.3150506485813	39.0	39.0	39.0	37.0	39.0
10	38.2118530760347	39.0	39.0	39.0	37.0	39.0
11	38.12336950674607	39.0	39.0	39.0	35.0	39.0
12	37.65560125408307	39.0	37.0	39.0	35.0	39.0
13	37.55737233615605	39.0	37.0	39.0	35.0	39.0
14	38.833515695904055	41.0	38.0	41.0	35.0	41.0
15	38.84292935448114	41.0	37.0	41.0	35.0	41.0
16	38.8824625241569	41.0	37.0	41.0	35.0	41.0
17	38.858869673483944	41.0	37.0	41.0	35.0	41.0
18	38.8665748827143	40.0	37.0	41.0	35.0	41.0
19	38.86369815222297	41.0	37.0	41.0	35.0	41.0
20	38.75917649795115	41.0	36.0	41.0	35.0	41.0
21	38.70085127830136	41.0	36.0	41.0	35.0	41.0
22	38.622110379131115	40.0	35.0	41.0	35.0	41.0
23	38.541100773511936	40.0	35.0	41.0	35.0	41.0
24	38.496885550857726	40.0	35.0	41.0	35.0	41.0
25	38.51537046387056	40.0	35.0	41.0	35.0	41.0
26	38.47285431574305	40.0	35.0	41.0	35.0	41.0
27	38.459085383628796	40.0	35.0	41.0	35.0	41.0
28	38.40729575280474	40.0	36.0	41.0	35.0	41.0
29	38.37797801202923	40.0	36.0	41.0	35.0	41.0
30	38.329780722959335	40.0	36.0	41.0	35.0	41.0
31	38.248364875138556	40.0	36.0	41.0	35.0	41.0
32	38.142708421222274	40.0	35.0	41.0	35.0	41.0
33	38.03612475296233	40.0	35.0	41.0	35.0	41.0
34	37.91470699446395	40.0	35.0	41.0	35.0	41.0
35	37.79204729552067	40.0	35.0	41.0	34.0	41.0
36	37.72917618099295	40.0	35.0	41.0	34.0	41.0
37	37.693082677430745	40.0	35.0	41.0	34.0	41.0
38	37.581202235849936	40.0	35.0	41.0	34.0	41.0
39	37.58516108842554	40.0	35.0	41.0	34.0	41.0
40	37.535638378370415	40.0	35.0	41.0	34.0	41.0
41	37.49917479262675	40.0	35.0	41.0	34.0	41.0
42	37.48436302338839	40.0	35.0	41.0	34.0	41.0
43	37.429827909553524	40.0	35.0	41.0	34.0	41.0
44	37.351376736964426	40.0	35.0	41.0	34.0	41.0
45	37.27569604690624	40.0	35.0	41.0	34.0	41.0
46	37.2283505271997	40.0	35.0	41.0	34.0	41.0
47	37.180101006989595	40.0	35.0	41.0	34.0	41.0
48	37.10566940009634	40.0	35.0	41.0	33.0	41.0
49	37.05821629316937	39.0	35.0	41.0	33.0	41.0
50	36.95333169943613	39.0	35.0	41.0	33.0	41.0
51	36.90593171648937	39.0	35.0	41.0	33.0	41.0
52	36.704539600355886	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	1.0
13	7.0
14	8.0
15	25.0
16	50.0
17	101.0
18	243.0
19	484.0
20	934.0
21	1516.0
22	2390.0
23	3827.0
24	6235.0
25	10006.0
26	13876.0
27	16481.0
28	16270.0
29	16902.0
30	18519.0
31	21821.0
32	27460.0
33	38083.0
34	91005.0
35	343256.0
36	88047.0
37	114848.0
38	236900.0
39	1166788.0
40	3953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.581383482370654	24.03976173671666	29.416176385899735	13.962678395012954
2	30.373747289672565	25.319424671758533	30.63284945869343	13.673978579875476
3	29.363275615691304	25.536741928614763	31.578411664418944	13.52157079127499
4	25.955394606264253	28.305349495523075	31.18949055888659	14.549765339326076
5	23.588386472938243	33.27195058309149	28.874043935763734	14.26561900820654
6	21.71596705956091	42.891899842994086	24.555912542750296	10.836220554694709
7	88.63561994122435	3.5390838479439903	5.965689051504815	1.8596071593268524
8	88.69700269146618	3.322346937090047	5.90796694527739	2.072683426166373
9	83.34161442436596	5.923011299336665	7.512043295597451	3.223330980699924
10	37.51119956179413	35.80891081108711	14.347537078529296	12.33235254858947
11	35.50052387387206	23.991726944527404	25.693301423231606	14.814447758368923
12	35.6254321903642	22.398900378251668	26.800244459592964	15.175422971791166
13	22.311625267907804	33.429983263714135	27.69237019110794	16.566021277270124
14	16.306561972247856	36.809025541027566	29.647734440812073	17.236678045912512
15	16.27598220212737	27.786921947480476	41.190727142291465	14.746368708100693
16	18.792273184041555	23.11531519707434	40.586363743910276	17.50604787497383
17	18.358174374331206	23.552003242794893	30.582582566495375	27.507239816378526
18	19.187979873600643	25.01563586056161	35.981005721765165	19.815378544072594
19	24.543100288699815	26.060303306677596	29.912907921856856	19.483688482765732
20	26.465474100273966	25.003180742512534	30.22477693508562	18.30656822212788
21	22.546710040834036	27.584916896684575	31.087394304484334	18.780978757997055
22	20.87942061826492	24.24524886352628	29.2627418312952	25.6125886869136
23	18.119696809391606	29.67072507090266	29.074307948552775	23.135270171152964
24	16.92021090666563	25.601963890871737	39.19719398243694	18.280631220025686
25	17.22774964587733	28.368473283771785	34.548801072122274	19.854975998228603
26	17.34810447835153	33.28458426914126	29.70871541305234	19.65859583945487
27	17.025030323078617	34.517328461998275	29.865632043670594	18.592009171252517
28	15.265019466144178	30.16678697685714	36.111672856279995	18.45652070071869
29	17.151054689575155	25.79035313161399	34.34246574730931	22.716126431501536
30	17.842023568297574	32.41562773571757	31.827335457399702	17.915013238585153
31	24.3290865398566	29.636573940768102	28.190708839071394	17.843630680303903
32	24.78849736366668	27.47058873423412	30.108618450627954	17.632295451471244
33	24.963627930356694	28.72083259116008	27.340546587721754	18.97499289076147
34	19.083562235189238	28.37494637379729	29.25484019726407	23.286651193749407
35	19.51346469688305	27.12425609686957	32.81021837527226	20.55206083097512
36	25.26741674155362	28.756456907300443	28.435793420037026	17.540332931108914
37	18.50388586290531	33.601185334388674	30.463566994026454	17.431361808679565
38	19.36828891231106	31.957868665914003	28.622039844770836	20.051802577004104
39	19.107624273284042	32.282549933193245	29.49050531619259	19.119320477330124
40	22.174842179368877	26.717210339265808	30.645259934742324	20.46268754662299
41	17.492610632920886	27.60143443674965	30.28633825332817	24.61961667700129
42	19.333914572175622	27.252601847375253	29.013862680314617	24.399620900134504
43	19.008027970891632	26.54029409256876	30.363301061631404	24.088376874908203
44	17.692026447706585	28.93252495599415	31.609616422541887	21.765832173757378
45	16.986548918927003	35.95547049766456	27.10465825879235	19.953322324616092
46	19.179810387568452	33.284003923138975	28.94663182804973	18.58955386124284
47	19.19431903762562	29.144083394827693	29.24961708324349	22.411980484303204
48	19.532392904957625	27.234745047304898	33.63904175053782	19.593820297199652
49	20.52411493886501	26.155346125052066	33.8860905795112	19.434448356571725
50	18.357906522330154	31.774836465192855	30.98436056807838	18.88289644439861
51	17.857425058358256	30.89364802372097	29.753312771228053	21.49561414669272
52	18.28255082603325	27.758886771370012	35.338607339234116	18.61995506336262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4919.0
1	5286.0
2	5653.0
3	11772.5
4	17892.0
5	11995.0
6	6098.0
7	6333.0
8	6568.0
9	6655.0
10	6742.0
11	6997.0
12	7252.0
13	7153.5
14	6881.0
15	6707.0
16	6384.0
17	6061.0
18	6325.5
19	6590.0
20	8001.0
21	9412.0
22	11314.5
23	13217.0
24	16358.5
25	19500.0
26	22296.0
27	25092.0
28	32732.0
29	40372.0
30	48403.5
31	56435.0
32	62993.5
33	69552.0
34	82718.0
35	95884.0
36	102871.0
37	109858.0
38	115634.5
39	140343.0
40	159275.0
41	186778.0
42	214281.0
43	218931.0
44	223581.0
45	222431.0
46	221281.0
47	206872.0
48	192463.0
49	170391.5
50	148320.0
51	131601.5
52	114883.0
53	98788.0
54	82693.0
55	72394.0
56	62095.0
57	55105.0
58	48115.0
59	44105.0
60	40095.0
61	34409.0
62	28723.0
63	25152.5
64	18089.0
65	14596.0
66	12359.0
67	10122.0
68	8087.5
69	6053.0
70	4886.5
71	3720.0
72	3185.0
73	2650.0
74	2137.0
75	1624.0
76	1187.0
77	750.0
78	564.5
79	379.0
80	324.5
81	270.0
82	193.5
83	117.0
84	96.5
85	76.0
86	49.0
87	22.0
88	13.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2240043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.545592894597426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96650418328919	10.904322505812592
2	10.218535709698372	2.9726932082435744
3	3.5270632710211567	1.5390967936128268
4	1.718603836573334	0.9999244701555585
5	1.0290743270989438	0.7484248110131511
6	0.6747829541227788	0.5889070885730251
7	0.48711697088140776	0.49597836053432975
8	0.3814662270885567	0.44389219538145586
9	0.32431754069681173	0.42456518239975705
>10	3.7853874944206973	13.248017135103762
>50	1.1367435698148345	11.740109206480119
>100	1.7318549511616537	53.34813767023837
>500	0.016694067719121467	1.5850184399768614
>1k	0.0015457470110297653	0.5116415061863575
>5k	3.091494022059531E-4	0.4492714262882666
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9991	0.4460182237573118	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4167	0.18602321473293146	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	2481	0.11075680243638181	TruSeq Adapter, Index 23 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2384	0.10642652841932052	Illumina PCR Primer Index 7 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11580134845625731	0.0
2	0.0	0.0	0.0	0.34807367537141026	0.0
3	0.0	0.0	0.0	0.47441053586917753	0.0
4	0.0	0.0	0.0	0.6347199585007967	0.0
5	0.0	0.0	0.0	1.0463192001224977	0.0
6	0.0	0.0	0.0	1.346268799304299	0.0
7	0.0	0.0	0.0	1.5465328120933393	0.0
8	0.0	0.0	0.0	1.9858547358242677	0.0
9	0.0	0.0	0.0	2.141342822436891	0.0
10	0.0	0.0	0.0	2.477273873760459	0.0
11	0.0	0.0	0.0	2.910211991466235	0.0
12	0.0	0.0	0.0	3.2535982568191772	0.0
13	0.0	0.0	0.0	3.3814083033227487	0.0
14	4.464200017588948E-5	0.0	0.0	3.4263627974998694	0.0
15	4.464200017588948E-5	0.0	0.0	3.5183699598623774	0.0
16	4.464200017588948E-5	0.0	0.0	3.7082323866104354	0.0
17	4.464200017588948E-5	0.0	0.0	3.949968817562877	0.0
18	4.464200017588948E-5	0.0	0.0	4.251793380752066	0.0
19	4.464200017588948E-5	0.0	0.0	4.405852923359061	0.0
20	4.464200017588948E-5	0.0	0.0	4.589867248084077	0.0
21	8.928400035177896E-5	0.0	0.0	4.831826889037398	0.0
22	8.928400035177896E-5	0.0	0.0	5.076018629999513	0.0
23	8.928400035177896E-5	0.0	0.0	5.348156263071735	0.0
24	4.910620019347843E-4	0.0	0.0	5.56315213591882	0.0
25	4.910620019347843E-4	0.0	0.0	5.735782750598984	0.0
26	4.910620019347843E-4	0.0	0.0	5.904395585263319	0.0
27	4.910620019347843E-4	0.0	0.0	6.067517453906019	0.0
28	5.357040021106737E-4	0.0	0.0	6.246353306610632	0.0
29	5.357040021106737E-4	0.0	0.0	6.435144325354469	0.0
30	5.357040021106737E-4	0.0	0.0	6.6663452442654005	0.0
31	5.357040021106737E-4	0.0	0.0	6.87509123708786	0.0
32	5.357040021106737E-4	0.0	0.0	7.066560775842249	0.0
33	5.357040021106737E-4	0.0	0.0	7.26084276060772	0.0
34	5.803460022865632E-4	0.0	0.0	7.469231617428773	0.0
35	5.803460022865632E-4	0.0	0.0	7.713021580389305	0.0
36	5.803460022865632E-4	0.0	0.0	7.944356425300764	0.0
37	5.803460022865632E-4	0.0	0.0	8.153638122125335	0.0
38	5.803460022865632E-4	0.0	0.0	8.371267872982795	0.0
39	5.803460022865632E-4	0.0	0.0	8.591397575850106	0.0
40	5.803460022865632E-4	0.0	0.0	8.824339532767898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	25	3.4182012E-5	46.0	28
CGAGTCA	25	3.4182012E-5	46.0	46
CGCGGAC	20	6.3127436E-4	46.0	39
CTATCGT	30	1.8621995E-6	46.0	24
GCGCGTT	30	1.8621995E-6	46.0	26
ATTACGC	25	3.4182012E-5	46.0	25
ATTCCCG	25	3.4182012E-5	46.0	30
AATGCGC	30	1.8621995E-6	46.0	23
TCGGACT	20	6.3127436E-4	46.0	39
TCTACGA	30	1.8621995E-6	46.0	29
GCGATCG	20	6.3127436E-4	46.0	9
TTAACGG	120	0.0	46.0	2
TCCAACG	25	3.4182012E-5	46.0	17
GTGCGTA	25	3.4182012E-5	46.0	16
ATCGCGA	20	6.3127436E-4	46.0	42
CCCGCGA	20	6.3127436E-4	46.0	33
TGCGTAA	25	3.4182012E-5	46.0	13
TGCCGAT	20	6.3127436E-4	46.0	23
CCGTAAA	35	1.0201438E-7	45.999996	39
TTCGCTA	45	3.110472E-10	45.999996	26
>>END_MODULE