FastQCFastQC Report
Thu 26 May 2016
SRR1544735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544735_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1014208
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63020.6213715529753265No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.38867766769735596No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23690.2335812772133527No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT21920.2161292358175049Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT18050.17797138259607498No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT11790.11624834353505395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC351.0194162E-746.0000046
GATCGAA551.8189894E-1246.00000415
GACAACT351.0194162E-746.0000049
ATCACGC551.8189894E-1246.00000424
GCCGCAC351.0194162E-746.0000049
ATGAGCG351.0194162E-746.0000041
TATTCGT551.8189894E-1246.00000414
TGTTCGC351.0194162E-746.00000446
GACGGTC700.046.00000429
ACTAAGG351.0194162E-746.0000042
GCGATGT1050.046.0000049
GGTTGCG351.0194162E-746.0000041
CGGTCTA700.046.00000431
CAGTCGC351.0194162E-746.00000412
GAACCGA351.0194162E-746.0000049
AGTAGTA351.0194162E-746.0000041
CGCAAAC551.8189894E-1246.00000428
TATACGA551.8189894E-1246.00000446
CCAAACG551.8189894E-1246.00000434
ACGAGCC351.0194162E-746.00000410