##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544735_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1014208
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72370361898151	33.0	31.0	34.0	31.0	34.0
2	33.11517952924844	34.0	33.0	34.0	31.0	34.0
3	33.14397046759639	34.0	33.0	34.0	31.0	34.0
4	36.44820194674071	37.0	37.0	37.0	35.0	37.0
5	36.43531701583896	37.0	37.0	37.0	35.0	37.0
6	36.51647393828485	37.0	37.0	37.0	35.0	37.0
7	36.62852491796554	37.0	37.0	37.0	35.0	37.0
8	36.632018284217835	37.0	37.0	37.0	35.0	37.0
9	38.39457586609453	39.0	39.0	39.0	37.0	39.0
10	38.27322699091311	39.0	39.0	39.0	37.0	39.0
11	38.02227748154225	39.0	39.0	39.0	35.0	39.0
12	36.832178409162616	39.0	35.0	39.0	35.0	39.0
13	36.62534904082791	38.0	35.0	39.0	35.0	39.0
14	37.534118247933364	39.0	35.0	41.0	35.0	41.0
15	37.6145139852969	39.0	35.0	41.0	35.0	41.0
16	37.73832389411245	39.0	35.0	41.0	35.0	41.0
17	37.72405265980943	39.0	35.0	41.0	35.0	41.0
18	37.81481609295135	38.0	36.0	41.0	35.0	41.0
19	37.757934269893354	38.0	36.0	41.0	35.0	41.0
20	37.56329470877769	38.0	35.0	41.0	35.0	41.0
21	37.46346114406512	38.0	35.0	41.0	35.0	41.0
22	37.39073148703225	38.0	35.0	41.0	35.0	41.0
23	37.35778065248943	37.0	35.0	41.0	35.0	41.0
24	37.30239063387392	37.0	35.0	41.0	35.0	41.0
25	37.292817646873225	37.0	35.0	41.0	35.0	41.0
26	37.244736779832145	37.0	35.0	41.0	35.0	41.0
27	37.221342170442355	37.0	35.0	41.0	35.0	41.0
28	37.2592771896889	36.0	35.0	41.0	35.0	41.0
29	37.258839409667445	36.0	35.0	41.0	35.0	41.0
30	37.24564882154351	36.0	35.0	41.0	35.0	41.0
31	37.07301263646116	36.0	35.0	41.0	35.0	41.0
32	36.9325542689468	36.0	35.0	41.0	34.0	41.0
33	36.80121927651921	36.0	35.0	41.0	34.0	41.0
34	36.68241721619234	36.0	35.0	41.0	34.0	41.0
35	36.5898799851707	36.0	35.0	41.0	34.0	41.0
36	36.491387368271596	36.0	35.0	41.0	34.0	41.0
37	36.44666084274626	36.0	35.0	41.0	33.0	41.0
38	36.36806848299363	35.0	35.0	41.0	33.0	41.0
39	36.39340943869502	36.0	35.0	41.0	33.0	41.0
40	36.28038627184956	36.0	35.0	41.0	33.0	41.0
41	36.2959018268442	36.0	35.0	41.0	33.0	41.0
42	36.306735896384176	36.0	35.0	41.0	33.0	41.0
43	36.241650627879096	35.0	35.0	41.0	33.0	41.0
44	36.133007233230266	35.0	35.0	41.0	33.0	41.0
45	36.08545880135041	35.0	35.0	40.0	33.0	41.0
46	36.03685733104057	35.0	35.0	40.0	33.0	41.0
47	36.004864879787974	35.0	35.0	40.0	33.0	41.0
48	35.99393418312614	35.0	35.0	40.0	33.0	41.0
49	36.03002737111125	36.0	35.0	40.0	33.0	41.0
50	35.87840857102291	35.0	35.0	40.0	33.0	41.0
51	35.810197710923205	35.0	35.0	40.0	33.0	41.0
52	35.60012344607812	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	6.0
15	13.0
16	34.0
17	54.0
18	143.0
19	288.0
20	468.0
21	792.0
22	1266.0
23	2171.0
24	3495.0
25	5556.0
26	7582.0
27	8601.0
28	8261.0
29	8556.0
30	9447.0
31	11311.0
32	15147.0
33	21652.0
34	62732.0
35	329831.0
36	39059.0
37	38851.0
38	74456.0
39	362897.0
40	1533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.04895642708399	24.84352322206096	30.336183504764307	13.771336846090742
2	28.92424433646747	26.97563024547233	30.593724364232976	13.506401053827222
3	27.41597384362971	25.41401763740771	32.87836420142614	14.291644317536441
4	24.234082160661323	28.49119707200101	32.51147693569761	14.763243831640057
5	21.29316668770114	34.372436423297785	30.587611219789235	13.746785669211839
6	20.89679828989714	41.982906859342464	26.52522953871395	10.595065312046444
7	88.0451544456364	4.964267684735281	5.269136114090995	1.7214417555373256
8	89.2770516501546	3.3211136177194422	5.273967470183631	2.127867261942323
9	85.73320265665426	4.884008014135167	6.899866694011485	2.482922635199091
10	56.408645958225534	25.042101817378683	10.378443080709282	8.170809143686503
11	54.56691329589197	15.885893229002335	18.77711475358112	10.770078721524579
12	49.5558110367893	19.255813403167792	20.41169069855493	10.776684861487979
13	20.04914179339938	49.179359657979425	19.967107338928503	10.804391209692685
14	11.744928062093772	52.74894301760585	24.232997570518076	11.273131349782293
15	10.205303054205842	23.27540307313687	55.675857417807784	10.843436454849497
16	11.717418912096928	18.323164479081214	54.223394017795165	15.736022591026694
17	12.089531930333818	18.16146194863381	28.481534359815736	41.26747176121663
18	17.523131349782293	22.524570896699693	41.085655013567234	18.866642739950777
19	29.930842588502554	22.779646778570076	26.06713810184893	21.222372531078438
20	33.12969331734713	21.78823278854042	27.83423124250647	17.247842651605982
21	18.91564649460466	31.854708304410927	30.780668265286803	18.44897693569761
22	20.46049725500095	23.78910440461917	24.034024578784628	31.716373761595257
23	14.26867072632044	33.03405218653373	24.13025730422162	28.567019782924213
24	15.310666056666877	21.979515050167226	46.3826946740708	16.327124219095097
25	11.880403230895437	24.60935035022402	44.72337035401022	18.786876064870324
26	12.186454849498327	38.748560453082604	27.59710039755159	21.46788429986748
27	13.035886129866853	44.53524326370922	27.093456174670283	15.335414431753644
28	10.078997602069792	32.55535353694706	41.57766454218464	15.787984318798513
29	11.463920615889442	24.43335094970657	40.734247807155924	23.368480627248058
30	14.6686872909699	38.880880450558465	29.38243437243642	17.06799788603521
31	29.902741843882126	30.657813781788352	24.206080015144824	15.233364359184703
32	32.72208462169496	25.64730311730927	27.7585071306872	13.872105130308576
33	31.412589922382782	29.203378399697105	21.975669685113903	17.40836199280621
34	17.16847037294125	30.45795339811952	24.407813781788352	27.965762447150883
35	15.924741275951284	26.858790307313683	33.044799488862246	24.171668927872783
36	33.96265854735912	24.31818719631476	26.128466744494226	15.590687511831893
37	16.868926295197827	37.979586041522055	30.201004133274438	14.950483530005679
38	15.856707894238657	38.715726951473464	22.979704360446775	22.447860793841105
39	17.774953461222946	37.21711917082098	28.426713258029913	16.58121410992617
40	25.28278223007509	26.36076623335647	24.813253297154038	23.5431982394144
41	14.725381775730423	24.17364090995141	27.938746292673695	33.16223102164447
42	19.01996434656402	22.01737710607686	27.414199059758943	31.548459487600176
43	19.40617703666309	22.39087051176879	28.926019120338236	29.276933331229888
44	14.956300877137629	29.750406228308197	32.48810894806588	22.805183946488295
45	14.097798479207421	45.4364390736417	22.33368303148861	18.132079415662268
46	20.858936233987503	37.98757256894049	24.66456584842557	16.488925348646433
47	17.140172430112955	30.514450684672177	25.09672572095665	27.24865116425822
48	19.7427943774847	25.007986527418442	35.340186628383925	19.909032466712944
49	21.398076134284093	22.96205512084306	36.327952451568116	19.311916293304726
50	18.993145390294693	37.68023916198649	26.22617845649019	17.100436991228623
51	15.311159052186534	37.42644506846722	23.675616835994195	23.586779043352056
52	17.893765381460213	27.991792610588757	36.9778191455796	17.136622862371425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7000.0
1	5718.5
2	4437.0
3	5781.0
4	7125.0
5	4827.0
6	2529.0
7	2742.0
8	2955.0
9	3189.0
10	3423.0
11	3508.0
12	3593.0
13	3478.5
14	3262.0
15	3160.0
16	2938.5
17	2717.0
18	2715.0
19	2713.0
20	2973.0
21	3233.0
22	3860.0
23	4487.0
24	6026.5
25	7566.0
26	7701.0
27	7836.0
28	8217.5
29	8599.0
30	10183.5
31	11768.0
32	14695.5
33	17623.0
34	20400.0
35	23177.0
36	25619.5
37	28062.0
38	34821.5
39	57098.0
40	72615.0
41	104344.5
42	136074.0
43	144679.5
44	153285.0
45	144024.0
46	134763.0
47	116966.0
48	99169.0
49	82179.0
50	65189.0
51	56671.5
52	48154.0
53	41081.0
54	34008.0
55	28738.0
56	23468.0
57	19890.0
58	16312.0
59	13925.5
60	11539.0
61	9912.5
62	8286.0
63	6823.0
64	4431.5
65	3503.0
66	3018.5
67	2534.0
68	1835.0
69	1136.0
70	903.5
71	671.0
72	523.5
73	376.0
74	404.0
75	432.0
76	324.0
77	216.0
78	145.0
79	74.0
80	79.0
81	84.0
82	44.5
83	5.0
84	4.5
85	4.0
86	2.5
87	1.0
88	0.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1014208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.22456346606901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33977431214285	11.317906120684654
2	10.985918816534381	3.345116365108188
3	4.041842906243838	1.8460588153797037
4	1.9569580735917635	1.191753295673358
5	1.0352185873133117	0.7880375541902909
6	0.694448031937333	0.6343600881669986
7	0.5044531277501252	0.5376055061362159
8	0.3733016592220114	0.4546683842251502
9	0.3085766542809649	0.4228150371523008
>10	3.110124742786807	11.35605622788783
>50	1.0097845169158013	11.020997274471176
>100	1.612922562779814	53.09216357335125
>500	0.02277220237890016	2.2303503022828775
>1k	0.0032531717684143088	1.1378590301262843
>5k	6.506343536828617E-4	0.6242524251637367
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6302	0.6213715529753265	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3942	0.38867766769735596	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2369	0.2335812772133527	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	2192	0.2161292358175049	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1805	0.17797138259607498	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1179	0.11624834353505395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22480595696346312	0.0
2	0.0	0.0	0.0	0.6710655013567237	0.0
3	0.0	0.0	0.0	0.8872933362781599	0.0
4	0.0	0.0	0.0	1.153116520477062	0.0
5	0.0	0.0	0.0	1.8892574304284722	0.0
6	0.0	0.0	0.0	2.3745622199785448	0.0
7	0.0	0.0	0.0	2.7257722281819903	0.0
8	0.0	0.0	0.0	3.488436297090932	0.0
9	0.0	0.0	0.0	3.7359200479586043	0.0
10	0.0	0.0	0.0	4.210674733388023	0.0
11	0.0	0.0	0.0	4.78432432006058	0.0
12	0.0	0.0	0.0	5.254937843124882	0.0
13	0.0	0.0	0.0	5.432810626617026	0.0
14	0.0	0.0	0.0	5.487237331987127	0.0
15	0.0	0.0	0.0	5.593527166025115	0.0
16	0.0	0.0	0.0	5.854617593235313	0.0
17	0.0	0.0	0.0	6.166880955385878	0.0
18	0.0	0.0	0.0	6.652382943143813	0.0
19	0.0	0.0	0.0	6.834298289897141	0.0
20	0.0	0.0	0.0	7.037609642203572	0.0
21	0.0	0.0	0.0	7.321673660629772	0.0
22	0.0	0.0	0.0	7.592722597337035	0.0
23	9.859910393134348E-4	0.0	0.0	7.920071622389096	0.0
24	0.0019719820786268695	0.0	0.0	8.145469173976148	0.0
25	0.002070581182558213	0.0	0.0	8.31377784438695	0.0
26	0.002070581182558213	0.0	0.0	8.51994857070739	0.0
27	0.002070581182558213	0.0	0.0	8.688651637533917	0.0
28	0.002070581182558213	0.0	0.0	8.877173124250646	0.0
29	0.002070581182558213	0.0	0.0	9.059482867419701	0.0
30	0.002070581182558213	0.0	0.0	9.35222360699186	0.0
31	0.002070581182558213	0.0	0.0	9.630075881870386	0.0
32	0.002070581182558213	0.0	0.0	9.852614059443427	0.0
33	0.002070581182558213	0.0	0.0	10.040149555120843	0.0
34	0.002169180286489556	0.0	0.0	10.252334826781095	0.0
35	0.002169180286489556	0.0	0.0	10.583430617782545	0.0
36	0.002169180286489556	0.0	0.0	10.813955322774026	0.0
37	0.002169180286489556	0.0	0.0	11.037676689594244	0.0
38	0.0022677793904208998	0.0	0.0	11.240692244588882	0.0
39	0.0022677793904208998	0.0	0.0	11.454849498327759	0.0
40	0.0023663784943522433	0.0	0.0	11.719095096863759	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	35	1.0194162E-7	46.000004	6
GATCGAA	55	1.8189894E-12	46.000004	15
GACAACT	35	1.0194162E-7	46.000004	9
ATCACGC	55	1.8189894E-12	46.000004	24
GCCGCAC	35	1.0194162E-7	46.000004	9
ATGAGCG	35	1.0194162E-7	46.000004	1
TATTCGT	55	1.8189894E-12	46.000004	14
TGTTCGC	35	1.0194162E-7	46.000004	46
GACGGTC	70	0.0	46.000004	29
ACTAAGG	35	1.0194162E-7	46.000004	2
GCGATGT	105	0.0	46.000004	9
GGTTGCG	35	1.0194162E-7	46.000004	1
CGGTCTA	70	0.0	46.000004	31
CAGTCGC	35	1.0194162E-7	46.000004	12
GAACCGA	35	1.0194162E-7	46.000004	9
AGTAGTA	35	1.0194162E-7	46.000004	1
CGCAAAC	55	1.8189894E-12	46.000004	28
TATACGA	55	1.8189894E-12	46.000004	46
CCAAACG	55	1.8189894E-12	46.000004	34
ACGAGCC	35	1.0194162E-7	46.000004	10
>>END_MODULE