FastQCFastQC Report
Thu 26 May 2016
SRR1544734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544734_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1069557
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62640.5856630361916195No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.37557605625506635No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT29860.2791810067158646RNA PCR Primer, Index 16 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT24560.22962778047359794RNA PCR Primer, Index 16 (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22430.209712993323404No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT12070.11285046051776576Illumina PCR Primer Index 7 (95% over 23bp)
AAATGGGGGAATGAAATGCTACTTTCATCTTTTGAACTGGGATTTTTCAAGG11520.10770814458696451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC551.8189894E-1246.0000047
TTATCGC551.8189894E-1246.00000427
CGTTCCA551.8189894E-1246.0000048
ACATAGT551.8189894E-1246.00000435
AATACGG750.046.0000042
CGGAATC253.4169963E-546.033
CGTGGCA206.3112564E-446.023
TGATAAT301.8612809E-646.038
ATCTCGA301.8612809E-646.038
ACACGTA501.6370905E-1146.016
AAATCGA253.4169963E-546.030
TCGCAAG650.046.01
GCGACTT301.8612809E-646.028
AATCATG405.6115823E-946.038
ATTAGAC253.4169963E-546.045
GTCGAAT253.4169963E-546.030
CGACGTG206.3112564E-446.020
CGAAACG253.4169963E-546.034
CGACGGT850.046.028
ACGTTCC206.3112564E-446.06
Produced by FastQC (version 0.11.5)