##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544734_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1069557
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.681193241687915	33.0	31.0	34.0	31.0	34.0
2	33.08099334584318	34.0	33.0	34.0	31.0	34.0
3	33.134374325071036	34.0	33.0	34.0	31.0	34.0
4	36.44771339909888	37.0	37.0	37.0	35.0	37.0
5	36.44537878766629	37.0	37.0	37.0	35.0	37.0
6	36.51553493642695	37.0	37.0	37.0	35.0	37.0
7	36.609158745162716	37.0	37.0	37.0	35.0	37.0
8	36.60472887372997	37.0	37.0	37.0	35.0	37.0
9	38.335210746131345	39.0	39.0	39.0	37.0	39.0
10	38.236944828559864	39.0	39.0	39.0	37.0	39.0
11	38.019528645972116	39.0	39.0	39.0	35.0	39.0
12	37.04288224003022	39.0	35.0	39.0	35.0	39.0
13	36.877154747245825	39.0	35.0	39.0	35.0	39.0
14	37.890787494261644	40.0	35.0	41.0	35.0	41.0
15	37.97518785815062	40.0	35.0	41.0	35.0	41.0
16	38.05969667815741	40.0	35.0	41.0	35.0	41.0
17	38.01010885815342	40.0	35.0	41.0	35.0	41.0
18	38.07863910011341	39.0	36.0	41.0	35.0	41.0
19	38.01810656187562	39.0	36.0	41.0	35.0	41.0
20	37.84697309259815	39.0	35.0	41.0	35.0	41.0
21	37.766585605068265	39.0	35.0	41.0	35.0	41.0
22	37.690327864714085	39.0	35.0	41.0	35.0	41.0
23	37.61904227638172	39.0	35.0	41.0	35.0	41.0
24	37.58803130641939	39.0	35.0	41.0	35.0	41.0
25	37.569880801116724	39.0	35.0	41.0	35.0	41.0
26	37.49742369971867	39.0	35.0	41.0	35.0	41.0
27	37.471628908043236	39.0	35.0	41.0	35.0	41.0
28	37.49973213208833	38.0	35.0	41.0	35.0	41.0
29	37.47551275902079	38.0	35.0	41.0	35.0	41.0
30	37.42284796415712	38.0	35.0	41.0	35.0	41.0
31	37.253748047088656	38.0	35.0	41.0	34.0	41.0
32	37.10273412263208	38.0	35.0	41.0	34.0	41.0
33	36.96394582055935	38.0	35.0	41.0	34.0	41.0
34	36.840242268528	38.0	35.0	41.0	34.0	41.0
35	36.72662420048675	38.0	35.0	41.0	34.0	41.0
36	36.6134642660466	38.0	35.0	41.0	33.0	41.0
37	36.573643106445004	38.0	35.0	41.0	33.0	41.0
38	36.486617356531724	38.0	35.0	41.0	33.0	41.0
39	36.52691347913201	38.0	35.0	41.0	33.0	41.0
40	36.36976523925326	37.0	35.0	41.0	32.0	41.0
41	36.3781425393878	37.0	35.0	41.0	33.0	41.0
42	36.393259078291294	37.0	35.0	41.0	33.0	41.0
43	36.32609762733543	37.0	35.0	41.0	33.0	41.0
44	36.200312839801896	37.0	35.0	41.0	33.0	41.0
45	36.11370688986188	37.0	35.0	41.0	33.0	41.0
46	36.08877039746362	36.0	35.0	41.0	33.0	41.0
47	36.03625239234562	36.0	35.0	40.0	33.0	41.0
48	36.0172641570295	36.0	35.0	40.0	33.0	41.0
49	36.02074036259872	36.0	35.0	40.0	33.0	41.0
50	35.87661714148942	36.0	35.0	40.0	32.0	41.0
51	35.80231254622241	36.0	35.0	40.0	32.0	41.0
52	35.55585069332443	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	5.0
12	2.0
13	7.0
14	12.0
15	26.0
16	35.0
17	89.0
18	173.0
19	321.0
20	620.0
21	947.0
22	1551.0
23	2486.0
24	4322.0
25	6654.0
26	8535.0
27	9479.0
28	9135.0
29	9511.0
30	10254.0
31	12286.0
32	15795.0
33	22532.0
34	62181.0
35	296611.0
36	43083.0
37	48597.0
38	92579.0
39	409975.0
40	1752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.220455758786112	24.92761021619231	30.860253357231077	13.991680667790495
2	28.211586666255283	26.993605763881686	31.02873432645478	13.766073243408252
3	28.402600328921224	25.990947654028723	31.571201908827675	14.035250108222378
4	24.77324724161499	28.339677081258873	32.213617413564684	14.673458263561457
5	23.692893412880288	33.44169595449331	28.349400733200753	14.516009899425649
6	20.669211645569145	42.18971031931912	25.836023699531673	11.305054335580058
7	85.88041591051248	5.458802102178752	6.509237001861519	2.151544985447246
8	87.22012945546615	3.7717484902627914	6.43107379971334	2.5770482545577282
9	83.7040943119441	5.3679233551835015	8.11775342501615	2.8102289078562435
10	53.63304620511109	24.383927177326687	12.029746895209886	9.953279722352338
11	50.458647832700834	18.82499015947724	19.69563099488854	11.020731012933393
12	44.94917054444036	21.461315292219115	21.497591993694588	12.091922169645937
13	19.831575128768268	46.16032619112399	21.90850978489225	12.099588895215497
14	12.099214908602345	48.906042408211995	26.00628110516784	12.988461578017816
15	11.077109494865631	25.272238880209287	51.827438836826836	11.82321278809825
16	13.165076756077514	18.64388714205975	51.36322795325541	16.82780814860732
17	13.280077639620892	18.740936668171962	29.174882685074287	38.80410300713286
18	18.729810566430775	22.4520058304513	39.44735998174945	19.370823621368473
19	28.25655855648647	23.353407064794116	27.70305836902568	20.686976009693733
20	31.041169381341994	22.40076966444986	28.2364567760297	18.32160417817844
21	19.036105602599953	30.840525563387462	30.661011989075853	19.462356844936735
22	20.499608716505993	23.728422141129457	25.274950283154613	30.497018859209934
23	14.918606488480746	31.505473761566705	26.257132625937658	27.318787124014897
24	14.811178833853642	22.077832223995543	45.70976581893251	17.401223123218305
25	14.2234588712897	25.06318036345889	41.34319162045594	19.37016914479546
26	13.871537468316323	37.93308818510842	27.458377627372826	20.736996719202434
27	13.761117920784024	42.170823995355086	28.235241319536968	15.83281676432392
28	12.053775535104721	32.201275855330756	40.6561782121009	15.088770397463621
29	12.85719227680245	25.859678352813358	37.28571735774718	23.997412012637007
30	15.791865230184085	36.169834800763304	30.648576934188643	17.389723034863966
31	29.541576559267064	29.061658237943373	25.11824989224511	16.27851531054446
32	30.410627951572472	25.360780210872353	28.510588963468052	15.718002874087123
33	28.692346457458555	28.789115493610907	23.644275153170895	18.874262895759646
34	17.693306668087814	28.947872810892733	26.66346908112424	26.69535143989521
35	17.91760513932404	26.32519818953081	31.77446363307425	23.982733038070904
36	32.798438979876714	24.98847653748234	25.149758264402926	17.063326218238018
37	17.3983247269664	36.797010351014485	29.572243461545295	16.232421460473823
38	17.084456461880947	35.75527064008744	24.466017238912933	22.69425565911868
39	19.321924871699217	34.11103849537706	26.946202960665023	19.6208336722587
40	25.94513429391795	24.190295608368697	25.864259688824436	24.000310408888915
41	14.313122161792219	25.035692347392423	28.54434125530477	32.10684423551059
42	19.95573868433379	22.79457756809595	26.882157753163227	30.36752599440703
43	20.610215257344862	23.134811889408418	29.085873871144784	27.169098982101936
44	16.0773105126702	29.171610302209235	32.292528588939156	22.45855059618141
45	14.54340441883883	42.97115534749434	22.81224843556725	19.673191798099587
46	20.532800028422983	34.802352749783324	25.971313356838394	18.693533864955302
47	17.884694317366908	29.069137970206356	27.284286858951884	25.76188085347485
48	20.537942344353784	24.81765815192645	35.321259175527814	19.32314032819195
49	22.51277865508804	23.589766604304398	34.43500439901754	19.462450341590024
50	20.187236397873136	36.54129700427373	25.387426757059234	17.8840398407939
51	16.908402263740967	35.04441558514413	24.541375541462493	23.505806609652407
52	18.950182178228932	27.224542497501304	35.75564462670059	18.06963069756918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6989.0
1	5691.5
2	4394.0
3	6086.0
4	7778.0
5	5507.0
6	3236.0
7	3292.0
8	3348.0
9	3534.0
10	3720.0
11	3887.5
12	4055.0
13	4148.5
14	4233.5
15	4225.0
16	4022.0
17	3819.0
18	3638.5
19	3458.0
20	3865.0
21	4272.0
22	4543.0
23	4814.0
24	5459.0
25	6104.0
26	7642.0
27	9180.0
28	11910.0
29	14640.0
30	13239.0
31	11838.0
32	16601.0
33	21364.0
34	27343.0
35	33322.0
36	32402.0
37	31482.0
38	35717.0
39	58003.0
40	76054.0
41	96617.0
42	117180.0
43	126217.5
44	135255.0
45	125393.0
46	115531.0
47	102239.0
48	88947.0
49	88588.0
50	88229.0
51	77478.0
52	66727.0
53	58169.0
54	49611.0
55	40145.0
56	30679.0
57	27199.0
58	23719.0
59	20307.0
60	16895.0
61	14322.5
62	11750.0
63	9713.0
64	6479.5
65	5283.0
66	4473.0
67	3663.0
68	2956.0
69	2249.0
70	1859.0
71	1469.0
72	1254.0
73	1039.0
74	887.5
75	736.0
76	512.0
77	288.0
78	238.0
79	188.0
80	126.5
81	65.0
82	52.5
83	40.0
84	30.5
85	21.0
86	16.5
87	12.0
88	11.0
89	7.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1069557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.23158430849079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37521699088043	12.234591893582632
2	10.488486257023535	3.4048949789864897
3	3.6735895188412018	1.7888453396957726
4	1.8099206663814391	1.1751151955220065
5	1.0317463930012165	0.8373439281490258
6	0.6627273204287418	0.6454268625047588
7	0.4797323097821875	0.5450770802215618
8	0.34608178874625556	0.44939645853345167
9	0.28724163287589244	0.4196148102840248
>10	3.3121845377257904	12.941162846498393
>50	1.0239957499200631	11.749943179213487
>100	1.4888168845643879	50.289880830871006
>500	0.016207959863064874	1.6090213739767059
>1k	0.0034731342563710447	1.321135739532056
>5k	5.788557093951742E-4	0.588549482428618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6264	0.5856630361916195	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4017	0.37557605625506635	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	2986	0.2791810067158646	RNA PCR Primer, Index 16 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2456	0.22962778047359794	RNA PCR Primer, Index 16 (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2243	0.209712993323404	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1207	0.11285046051776576	Illumina PCR Primer Index 7 (95% over 23bp)
AAATGGGGGAATGAAATGCTACTTTCATCTTTTGAACTGGGATTTTTCAAGG	1152	0.10770814458696451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29301851140238433	0.0
2	0.0	0.0	0.0	0.8059411513364879	0.0
3	0.0	0.0	0.0	1.0568861687595892	0.0
4	0.0	0.0	0.0	1.3105425891280222	0.0
5	0.0	0.0	0.0	1.9885803187674898	0.0
6	0.0	0.0	0.0	2.433437395108442	0.0
7	0.0	0.0	0.0	2.745622720434722	0.0
8	0.0	0.0	0.0	3.4289897593115652	0.0
9	0.0	0.0	0.0	3.6729225277381197	0.0
10	0.0	0.0	0.0	4.14825951305073	0.0
11	0.0	0.0	0.0	4.766085397973179	0.0
12	0.0	0.0	0.0	5.263581090114879	0.0
13	0.0	0.0	0.0	5.468806244080493	0.0
14	0.0	0.0	0.0	5.542107620257733	0.0
15	0.0	0.0	0.0	5.6567345171879575	0.0
16	0.0	0.0	0.0	5.914972273567468	0.0
17	0.0	0.0	0.0	6.2371617407954885	0.0
18	0.0	0.0	0.0	6.743165628386332	0.0
19	9.349665328729558E-5	0.0	0.0	6.948297285698659	0.0
20	9.349665328729558E-5	0.0	0.0	7.173717716774328	0.0
21	9.349665328729558E-5	0.0	0.0	7.458228032727568	0.0
22	9.349665328729558E-5	0.0	0.0	7.726656924315394	0.0
23	9.349665328729558E-5	0.0	0.0	8.038187773068662	0.0
24	6.544765730110691E-4	0.0	0.0	8.267067580315963	0.0
25	6.544765730110691E-4	0.0	0.0	8.461353625846963	0.0
26	6.544765730110691E-4	0.0	0.0	8.641708670038156	0.0
27	6.544765730110691E-4	0.0	0.0	8.8275800167733	0.0
28	6.544765730110691E-4	0.0	0.0	9.011207443829548	0.0
29	6.544765730110691E-4	0.0	0.0	9.200444670083034	0.0
30	6.544765730110691E-4	0.0	0.0	9.467377615218263	0.0
31	6.544765730110691E-4	0.0	0.0	9.731225170795012	0.0
32	6.544765730110691E-4	0.0	0.0	9.943462573757172	0.0
33	6.544765730110691E-4	0.0	0.0	10.151212137361544	0.0
34	6.544765730110691E-4	0.0	0.0	10.372425219039284	0.0
35	6.544765730110691E-4	0.0	0.0	10.680870678234072	0.0
36	6.544765730110691E-4	0.0	0.0	10.913303358306289	0.0
37	6.544765730110691E-4	0.0	0.0	11.1420896689003	0.0
38	6.544765730110691E-4	0.0	0.0	11.344603419920585	0.0
39	6.544765730110691E-4	0.0	0.0	11.557775789415619	0.0
40	6.544765730110691E-4	0.0	0.0	11.792171899206869	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCC	55	1.8189894E-12	46.000004	7
TTATCGC	55	1.8189894E-12	46.000004	27
CGTTCCA	55	1.8189894E-12	46.000004	8
ACATAGT	55	1.8189894E-12	46.000004	35
AATACGG	75	0.0	46.000004	2
CGGAATC	25	3.4169963E-5	46.0	33
CGTGGCA	20	6.3112564E-4	46.0	23
TGATAAT	30	1.8612809E-6	46.0	38
ATCTCGA	30	1.8612809E-6	46.0	38
ACACGTA	50	1.6370905E-11	46.0	16
AAATCGA	25	3.4169963E-5	46.0	30
TCGCAAG	65	0.0	46.0	1
GCGACTT	30	1.8612809E-6	46.0	28
AATCATG	40	5.6115823E-9	46.0	38
ATTAGAC	25	3.4169963E-5	46.0	45
GTCGAAT	25	3.4169963E-5	46.0	30
CGACGTG	20	6.3112564E-4	46.0	20
CGAAACG	25	3.4169963E-5	46.0	34
CGACGGT	85	0.0	46.0	28
ACGTTCC	20	6.3112564E-4	46.0	6
>>END_MODULE