FastQCFastQC Report
Thu 26 May 2016
SRR1544733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544733_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1457570
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73000.5008335791763002No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66670.4574051332011499No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA38970.2673628024726085No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT28560.19594256193527582Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21550.14784881686642837No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT20910.1434579471311841No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT14890.10215632868404263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAC551.8189894E-1246.00000425
TCGATGG1050.046.0000042
TATTGCG1050.046.0000041
TTACGTC551.8189894E-1246.00000417
CGTCCAA551.8189894E-1246.00000420
CGATCAT551.8189894E-1246.00000410
TACGTCC551.8189894E-1246.00000418
TCACGGT301.8617484E-646.013
GCCCCGC206.312014E-446.011
TCGCCAC253.41761E-546.024
AACGTTC501.6370905E-1146.033
TCGTTAT253.41761E-546.023
GTCGACG301.8617484E-646.01
GATCGAC206.312014E-446.09
CTATCGT206.312014E-446.010
TTAGCCG301.8617484E-646.026
TAGCGTG206.312014E-446.036
CGTGAGA206.312014E-446.044
ATTACGC501.6370905E-1146.011
GCACGTC206.312014E-446.014
Produced by FastQC (version 0.11.5)