##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544733_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1457570
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.677004877981844	33.0	31.0	34.0	31.0	34.0
2	33.0804722929259	34.0	33.0	34.0	31.0	34.0
3	33.12358102869845	34.0	33.0	34.0	31.0	34.0
4	36.44306139670822	37.0	37.0	37.0	35.0	37.0
5	36.44739806664517	37.0	37.0	37.0	35.0	37.0
6	36.51032128817141	37.0	37.0	37.0	35.0	37.0
7	36.614250430511056	37.0	37.0	37.0	35.0	37.0
8	36.616714120076566	37.0	37.0	37.0	35.0	37.0
9	38.33640991513272	39.0	39.0	39.0	37.0	39.0
10	38.22980028403438	39.0	39.0	39.0	37.0	39.0
11	38.05288733988762	39.0	39.0	39.0	35.0	39.0
12	37.289057815405094	39.0	35.0	39.0	35.0	39.0
13	37.15759997804565	39.0	35.0	39.0	35.0	39.0
14	38.27542828131754	40.0	35.0	41.0	35.0	41.0
15	38.3105538670527	40.0	35.0	41.0	35.0	41.0
16	38.39798225814197	40.0	35.0	41.0	35.0	41.0
17	38.37280336450394	40.0	35.0	41.0	35.0	41.0
18	38.41109106252187	40.0	36.0	41.0	35.0	41.0
19	38.365084352724054	40.0	36.0	41.0	35.0	41.0
20	38.22771050446977	40.0	35.0	41.0	35.0	41.0
21	38.156981825915736	40.0	35.0	41.0	35.0	41.0
22	38.07720178104653	40.0	35.0	41.0	35.0	41.0
23	38.03916655803838	40.0	35.0	41.0	35.0	41.0
24	37.97860823150861	40.0	35.0	41.0	35.0	41.0
25	37.964025741473826	40.0	35.0	41.0	35.0	41.0
26	37.891273146401204	40.0	35.0	41.0	35.0	41.0
27	37.874862956839124	40.0	35.0	41.0	35.0	41.0
28	37.86819981201589	40.0	35.0	41.0	35.0	41.0
29	37.83568473555301	40.0	35.0	41.0	35.0	41.0
30	37.78803487997146	40.0	35.0	41.0	35.0	41.0
31	37.66523803316479	40.0	35.0	41.0	35.0	41.0
32	37.54577756128351	40.0	35.0	41.0	35.0	41.0
33	37.405880335078244	40.0	35.0	41.0	34.0	41.0
34	37.27117119589454	40.0	35.0	41.0	34.0	41.0
35	37.15932613871032	40.0	35.0	41.0	34.0	41.0
36	37.04045225958273	39.0	35.0	41.0	34.0	41.0
37	36.99859697990491	39.0	35.0	41.0	33.0	41.0
38	36.9057650747477	39.0	35.0	41.0	33.0	41.0
39	36.93167395047922	39.0	35.0	41.0	33.0	41.0
40	36.799226109209165	39.0	35.0	41.0	33.0	41.0
41	36.79132322975912	39.0	35.0	41.0	33.0	41.0
42	36.80249456286833	39.0	35.0	41.0	33.0	41.0
43	36.72858456197644	39.0	35.0	41.0	33.0	41.0
44	36.63004521223681	39.0	35.0	41.0	33.0	41.0
45	36.554848137653764	38.0	35.0	41.0	33.0	41.0
46	36.48968351434237	38.0	35.0	41.0	33.0	41.0
47	36.4151114526232	38.0	35.0	41.0	33.0	41.0
48	36.39050954671131	38.0	35.0	41.0	33.0	41.0
49	36.37164870297824	38.0	35.0	41.0	33.0	41.0
50	36.21928140672489	38.0	35.0	40.0	33.0	41.0
51	36.122716576219325	37.0	35.0	40.0	32.0	41.0
52	35.87377004191909	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	7.0
14	7.0
15	19.0
16	42.0
17	86.0
18	179.0
19	370.0
20	747.0
21	1247.0
22	1971.0
23	3288.0
24	5341.0
25	8658.0
26	11539.0
27	12548.0
28	11977.0
29	12217.0
30	13347.0
31	16053.0
32	20322.0
33	28777.0
34	73191.0
35	329563.0
36	58927.0
37	66817.0
38	134407.0
39	643337.0
40	2579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.418134292006556	24.483626858401312	28.728157138250648	14.370081711341479
2	29.096852981331946	27.615483304403902	30.583574030749812	12.704089683514342
3	28.338536056587333	25.6740328080298	32.35549579093971	13.63193534444315
4	24.16700398608643	29.09191325287979	31.873529230157043	14.867553530876734
5	22.837393744382776	34.22408529264461	28.536811268069457	14.401709694903161
6	20.36780394766632	42.56673778960873	26.315237004054694	10.750221258670251
7	87.9336841455299	4.5534691301275405	5.790733892711843	1.7221128316307277
8	88.75223831445489	3.676049863814431	5.587930596815247	1.9837812249154416
9	84.22175264309776	5.407081649594874	7.424892114958458	2.9462735923489096
10	46.09905527693353	31.573989585405847	12.232482831013263	10.094472306647365
11	41.266011237882225	21.017652668482476	24.056957813346873	13.659378280288426
12	41.133599072428765	20.53644078843555	24.734318077347915	13.59564206178777
13	20.21837716198879	43.554957909397146	23.426524969641253	12.800139958972812
14	13.363063180499049	44.42874098671076	26.241689935989353	15.96650589680084
15	11.762591162002511	26.32511646095899	48.175662232345616	13.73663014469288
16	14.000562580184827	20.833304746941828	46.633026201143	18.533106471730346
17	13.105648442270354	21.662973304884154	30.20252886653814	35.02884938630735
18	16.918638555952718	24.168581954897537	38.7601281585104	20.15265133063935
19	24.66824920930041	26.777170221670314	27.779660668098273	20.774919900931003
20	28.204683137001997	23.14468601851026	29.26940044045912	19.381230404028624
21	18.279053493142698	30.623846539102757	31.37660626933869	19.720493698415854
22	19.31968962039559	24.446784717029026	26.42329356394547	29.810232098629914
23	15.019518788119953	31.302647557235673	26.52613596602564	27.151697688618732
24	14.464828447347298	24.309570037802644	43.86869927344827	17.356902241401787
25	13.575540111281104	28.29387267849914	37.73431121661395	20.396275993605794
26	14.115411266697311	36.5155018283856	28.180396138778928	21.18869076613816
27	13.354898907085081	39.59844124124399	29.28662088270203	17.7600389689689
28	12.659700734784607	32.60303107226411	36.484834347578506	18.252433845372778
29	12.7345513423026	24.23300424679432	37.53342892622653	25.49901548467655
30	15.509992659014662	35.85920401764581	31.43224682176499	17.19855650157454
31	25.0764628799989	31.64266553235865	26.712061856377396	16.56880973126505
32	27.154030338165576	27.60491777410347	27.43827054618303	17.80278134154792
33	27.054824125084902	30.798315003739102	23.713989722620525	18.43287114855547
34	18.29181445831075	29.37087069574703	26.115521038440694	26.221793807501527
35	17.350933402855436	27.34105394595114	32.04998730764217	23.258025343551253
36	29.293550224002963	28.652208813298845	25.71595189253346	16.338289070164727
37	16.78135526938672	36.59542937903497	30.703225231035212	15.919990120543096
38	17.133996994998522	34.65864418175457	27.96387137495969	20.24348744828722
39	19.270017906515637	35.16290812791152	28.00222287780347	17.56485108776937
40	24.458996823480174	26.99053904786734	27.55586352628004	20.994600602372444
41	15.022125867025254	27.641622700796532	29.850504606982852	27.485746825195363
42	18.299429873007814	26.262820996590214	27.452540872822574	27.985208257579398
43	19.343496367241368	26.84646363467964	28.823795769671438	24.986244228407557
44	15.43754330838313	30.519357560871864	31.10608752924388	22.93701160150113
45	15.01128590736637	42.15077148953395	22.977764361231365	19.860178241868315
46	20.19628559863334	35.389243741295445	26.813532111665307	17.60093854840591
47	18.38381690073204	30.12829572507667	27.36341993866504	24.124467435526253
48	18.503193671658995	27.621657964969092	33.63310166921657	20.24204669415534
49	20.61554505100956	26.561468745926437	33.30577605192204	19.51721015114197
50	18.713269345554586	35.29463421996885	28.065273022907988	17.92682341156857
51	16.415060683191886	32.919448122560155	27.140857728959844	23.524633465288115
52	17.557235673072306	28.421962581556976	34.90988425941807	19.110917485952648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7924.0
1	6546.5
2	5169.0
3	8576.5
4	11984.0
5	8100.5
6	4217.0
7	4387.5
8	4558.0
9	4895.5
10	5233.0
11	5566.0
12	5899.0
13	5978.5
14	5842.0
15	5626.0
16	5572.0
17	5518.0
18	4869.5
19	4221.0
20	5072.5
21	5924.0
22	7248.0
23	8572.0
24	9357.5
25	10143.0
26	12773.0
27	15403.0
28	18941.5
29	22480.0
30	24444.0
31	26408.0
32	34798.5
33	43189.0
34	51358.5
35	59528.0
36	61380.5
37	63233.0
38	68213.0
39	90332.5
40	107472.0
41	126213.5
42	144955.0
43	155531.0
44	166107.0
45	155387.0
46	144667.0
47	137949.0
48	131231.0
49	120169.0
50	109107.0
51	94415.0
52	79723.0
53	68242.0
54	56761.0
55	47566.5
56	38372.0
57	32434.0
58	26496.0
59	22836.0
60	19176.0
61	16447.5
62	13719.0
63	11034.0
64	7311.5
65	6274.0
66	4991.5
67	3709.0
68	3228.5
69	2748.0
70	2299.0
71	1850.0
72	1418.5
73	987.0
74	813.0
75	639.0
76	479.0
77	319.0
78	253.0
79	187.0
80	150.5
81	114.0
82	109.0
83	104.0
84	61.0
85	18.0
86	10.5
87	3.0
88	2.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1457570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.41086049359498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36809679563825	11.460763648917489
2	10.761848132143486	3.316986804354379
3	3.96395484112523	1.832638651784741
4	1.9869113442610775	1.2248005415819494
5	1.1452149129189728	0.8824373629089407
6	0.7381318091083623	0.6825147801632311
7	0.5578628505632449	0.6018002595212574
8	0.39851356072106603	0.4913149511262513
9	0.32906160295698955	0.45640102112720166
>10	3.3208517255210213	11.59207772056792
>50	0.9281033600368563	10.070615861014875
>100	1.4759172640790024	53.26991246537235
>500	0.021500463938024923	2.1282424144355256
>1k	0.0031354843242953007	1.02536125406329
>5k	8.958526640843716E-4	0.9641322630605879
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7300	0.5008335791763002	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6667	0.4574051332011499	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	3897	0.2673628024726085	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	2856	0.19594256193527582	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2155	0.14784881686642837	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	2091	0.1434579471311841	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1489	0.10215632868404263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20685112893377333	0.0
2	0.0	0.0	0.0	0.5459086013021673	0.0
3	0.0	0.0	0.0	0.7204456732781273	0.0
4	0.0	0.0	0.0	0.9564549215475071	0.0
5	0.0	0.0	0.0	1.5811933560652318	0.0
6	0.0	0.0	0.0	2.0264549901548468	0.0
7	6.860733961319182E-5	0.0	0.0	2.318722256907044	0.0
8	6.860733961319182E-5	0.0	0.0	2.965483647440603	0.0
9	6.860733961319182E-5	0.0	0.0	3.189692433296514	0.0
10	6.860733961319182E-5	0.0	0.0	3.6536838711005304	0.0
11	6.860733961319182E-5	0.0	0.0	4.2837050707684705	0.0
12	6.860733961319182E-5	0.0	0.0	4.752842059043476	0.0
13	6.860733961319182E-5	0.0	0.0	4.927310523679823	0.0
14	6.860733961319182E-5	0.0	0.0	4.985626762351036	0.0
15	6.860733961319182E-5	0.0	0.0	5.099309124090095	0.0
16	6.860733961319182E-5	0.0	0.0	5.356449432960338	0.0
17	6.860733961319182E-5	0.0	0.0	5.665731319936607	0.0
18	6.860733961319182E-5	0.0	0.0	6.08601988240702	0.0
19	6.860733961319182E-5	0.0	0.0	6.287176602152899	0.0
20	6.860733961319182E-5	0.0	0.0	6.495262663199709	0.0
21	6.860733961319182E-5	0.0	0.0	6.783825133612794	0.0
22	6.860733961319182E-5	0.0	0.0	7.076847081100736	0.0
23	6.860733961319182E-5	0.0	0.0	7.460910968255384	0.0
24	2.0582201883957547E-4	0.0	0.0	7.699733117448905	0.0
25	2.0582201883957547E-4	0.0	0.0	7.894440747271143	0.0
26	2.0582201883957547E-4	0.0	0.0	8.084002826622392	0.0
27	2.0582201883957547E-4	0.0	0.0	8.27116364908718	0.0
28	2.0582201883957547E-4	0.0	0.0	8.488237271623317	0.0
29	2.0582201883957547E-4	0.0	0.0	8.69399068312328	0.0
30	2.0582201883957547E-4	0.0	0.0	8.966910680104558	0.0
31	2.0582201883957547E-4	0.0	0.0	9.217739113730387	0.0
32	2.0582201883957547E-4	0.0	0.0	9.433166160115809	0.0
33	2.0582201883957547E-4	0.0	0.0	9.679672331346007	0.0
34	2.744293584527673E-4	0.0	0.0	9.90285200710772	0.0
35	2.744293584527673E-4	0.0	0.0	10.221052848233704	0.0
36	2.744293584527673E-4	0.0	0.0	10.446427958863039	0.0
37	2.744293584527673E-4	0.0	0.0	10.68415239062275	0.0
38	2.744293584527673E-4	0.0	0.0	10.904999416837613	0.0
39	2.744293584527673E-4	0.0	0.0	11.128453521957779	0.0
40	2.744293584527673E-4	0.0	0.0	11.370637430792346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAC	55	1.8189894E-12	46.000004	25
TCGATGG	105	0.0	46.000004	2
TATTGCG	105	0.0	46.000004	1
TTACGTC	55	1.8189894E-12	46.000004	17
CGTCCAA	55	1.8189894E-12	46.000004	20
CGATCAT	55	1.8189894E-12	46.000004	10
TACGTCC	55	1.8189894E-12	46.000004	18
TCACGGT	30	1.8617484E-6	46.0	13
GCCCCGC	20	6.312014E-4	46.0	11
TCGCCAC	25	3.41761E-5	46.0	24
AACGTTC	50	1.6370905E-11	46.0	33
TCGTTAT	25	3.41761E-5	46.0	23
GTCGACG	30	1.8617484E-6	46.0	1
GATCGAC	20	6.312014E-4	46.0	9
CTATCGT	20	6.312014E-4	46.0	10
TTAGCCG	30	1.8617484E-6	46.0	26
TAGCGTG	20	6.312014E-4	46.0	36
CGTGAGA	20	6.312014E-4	46.0	44
ATTACGC	50	1.6370905E-11	46.0	11
GCACGTC	20	6.312014E-4	46.0	14
>>END_MODULE