FastQCFastQC Report
Thu 26 May 2016
SRR1544732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544732_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1925835
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92450.4800515101241799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53080.27562070478519707No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT32220.16730405252786454Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT24280.12607518297258072No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA21550.11189951371742646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC650.046.06
GGTCGTA206.312523E-446.08
GATCGAC206.312523E-446.09
ACGTTAG253.4180222E-546.01
TCCGAAC253.4180222E-546.021
ATAGCGC206.312523E-446.010
GCACGGA253.4180222E-546.033
CTAAGCG405.6152203E-946.01
CGACAGG1250.046.02
TAACGCG253.4180222E-546.01
TAGCCGA405.6152203E-946.033
ATCCGAT206.312523E-446.025
TGCGCAC650.046.030
CTACGAT301.8620631E-646.031
TATTCGA405.6152203E-946.026
ACAATCG206.312523E-446.026
ACTACGG650.046.02
CGAATTA206.312523E-446.033
ACGCTAG206.312523E-446.040
ACGTCGT501.6370905E-1146.046
Produced by FastQC (version 0.11.5)